LOCUS       sequence-324049-        2908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express  ReBphP-PCM and sfGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati 
            V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC
  TITLE     Multiplexed whole-animal imaging with reversibly switchable 
            optoacoustic proteins.
  JOURNAL   Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. 
            eCollection 2020 Jun.
  PUBMED    32582850
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-06-12"; volume: "6(24):eaaz6293. doi"; pages: " 
            10.1126/sciadv.aaz6293. eCollection 2020 Jun"
COMMENT     Sequence Label: pCDNA3_ReBphP-PCM_IRES_sfGFP
FEATURES             Location/Qualifiers
     source          1..2908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2181..2183,2184..2186,2187..2900)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2226..2247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     2834..2855
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gaattcgcca ccatgagcgg caccagagaa cccatggacc tgaccaactg cgacagagag
       61 cctatccatc agctgggcgc tatccagcct ttcggcttcc tgctgcaagt gtccagcgac
      121 tggatcgtga caagagcctc cgtgaacctg gccgagtttc tgggagtgac acaggccgat
      181 gctctgggca gacctgccag cacactgatc atgcctgagg ctctgcacac catccggaac
      241 aagctgacca cactgagagg cgccgacgtg gtggaaagag tgttcgccat tgctctgacc
      301 cctgaccaga gcaagttcga cgtggccgtg cacttcaacg agagccaagt gatcatcgag
      361 ggcgagagat gccaagagga cagaagaaac gcccctagcc tgagcatgcg gagcatgatg
      421 agcagactgg accaggccga gacacttgag gcctttttca gagagggcgc cagacaggct
      481 agagccctga ccggattcga cagagtgatg gtgtacagat tcgacgaagg cggctctggc
      541 gaagtggtgg ctgaagctgc tagaagcggc atcggcagct ttctgggcct gcactatcct
      601 gccagcgaca tccctgtgca ggccagagca ctgtacctgc ggaacctgtt cagaatcatt
      661 gccgatgtgg acgccgtgcc tgtgccaatt ctgcccgaga gagatgagca cggccagcct
      721 ctggatctga gcatgagcgt gctgagaagc gtgtccccta tccacatcga gtacctgaag
      781 aacatgggcg tgggcgccag cctgtctatc agcattgtgg tggatggcaa gctgtggggc
      841 ctgttcgcct gtcaccacta cgaacctaga ctgcccagcg ctcagagcag atctaccgcc
      901 gaactgtttg gccagatgtt cgccagcagg ctggaaagca gagagagaag gctggccctg
      961 gactacgaga caaaggccag aagaatcgcc gaccggctgc tgacatccgt ggccgataat
     1021 gcctctctgc tggacgatcc tgcctggctg attgaagccc tggccgatgc cattcctgcc
     1081 gatggaatcg gcgtgtggat caacggtaga ctggctctgg ccggcatcgg acccgacaag
     1141 aagaattttg ccagcctcgt gcggcacctg aacagaaatg ctggcggcag aatctacgcc
     1201 gtggacagac tgagccagac ataccccgac ctggaaatcg atgctgtggt ggccggaatg
     1261 ctggctatcc ccatcagcag atccccacgg gattacgtgg tgctgttccg gcaagaactc
     1321 gtgcggacag ttagatgggg cggagatcct cacaagcccg tggaatacgg ccctaatggc
     1381 cctagactga cccctcggaa gtccttcgaa gagtggagcg aacttgtgcg gggcagaagc
     1441 ctgcctttta ccgaggctga acagagagtg gccgaaacca tcagagtgac cctgatcgag
     1501 gtggtgctga gactgacaga cgaggcctct atggccagac agatggccaa tgagagacaa
     1561 gagctgctga tcgccgagct gaactaactc gagtactccg gtattgcggt acccttgtac
     1621 gcctgtttta tactcccttc ccgtaactta gacgcacaaa accaagttca atagaagggg
     1681 gtacaaacca gtaccaccac gaacaagcac ttctgtttcc ccggtgatgt cgtatagact
     1741 gcttgcgtgg ttgaaagcga cggatccgtt atccgcttat gtacttcgag aagcccagta
     1801 ccacctcgga atcttcgatg cgttgcgctc agcactcaac cccagagtgt agcttaggct
     1861 gatgagtctg gacatccctc accggtgacg gtggtccagg ctgcgttggc ggcctaccta
     1921 tggctaacgc catgggacgc tagttgtgaa caaggtgtga agagcctatt gagctacata
     1981 agaatcctcc ggcccctgaa tgcggctaat cccaacctcg gagcaggtgg tcacaaacca
     2041 gtgattggcc tgtcgtaacg cgcaagtccg tggcggaacc gactactttg ggtgtccgtg
     2101 tttcctttta ttttattgtg gctgcttatg gtgacaatca cagattgtta tcataaagcg
     2161 aattggatag gatccgtacg atggtgagca agggcgagga gctgttcacc ggggtggtgc
     2221 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg cgcggcgagg
     2281 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2341 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     2401 gctaccccga ccacatgaag cgccacgact tcttcaagtc cgccatgccc gaaggctacg
     2461 tccaggagcg caccatcagc ttcaaggacg acggcaccta caagacccgc gccgaggtga
     2521 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2581 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac gtctatatca
     2641 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacgtggagg
     2701 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2761 tgctgctgcc cgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     2821 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     2881 tggacgagct gtacaagtga gcggccgc
//
