LOCUS       sequence-32406-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBPHLWL-VP16AD
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    41..110
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     300..316
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     301..317
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             470..490
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             503..736
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 attgggtacc gggccccccc tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt
       61 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca
      121 tgatgaacgg gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc
      181 cctcgccctc gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt
      241 cggcgggtgc ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt
      301 cgaggtcgac ggtatcgata agcttaccga agtatacact taaattcagt gcacgtttgc
      361 ttgttgagag gaaaggttgt gtgcggacga attttttttt gaaaacatta acccttacgt
      421 ggatccgcgg ccgcaaaaca tggataaagc ggaattaatt cccgagcctc caaaaaagaa
      481 gagaaaggtc gaattgggta ccgccccccc gaccgatgtc agcctggggg acgagctcca
      541 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
      601 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
      661 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
      721 tgacgagtac ggtggggcta gcgga
//
