LOCUS       sequence-324061-       14363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at 
            ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14363)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 14363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pWZ157
FEATURES             Location/Qualifiers
     source          1..14363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1507..1540)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1540..1551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2973..3296)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5214..5238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5241..6266
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5241..6266
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6677)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10059..10139
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      10148..10471
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(10504..10520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10504..10520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(11993..12013)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(12150..12738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12230..12249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12909..13700,13701..13769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13532..13551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13770..13874)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13942..13960
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(13998..14020)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    14343..14363
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcctact gcgcatggaa
      181 ataggcggta gtcaggcagg tagatagcct cataaaattc aatacttcct tttcagaaag
      241 aatgctggaa tgtattcaaa gaggagtcac tcttcccatt gctcagagaa tatgaagagt
      301 tgctcggcga gcaaaagttc atgattggag atcgatattc atgggctgac atcgctgtcg
      361 tcgagtttct aactcggtgt cagcagtgct acgatagctt ctatctggct catttcgaga
      421 ggctccgaac tttttgccag acttttgagt ctcttccgca cattcgtccc tacattcagg
      481 gacgtgttga ttcatttatc taatttttta aaatggatat ttttcgtttt ttatttgttt
      541 gttttgctga taattcaata aagttacgca acgaatttta gacatttatg aaaaccgaaa
      601 atcgacaaaa atattttaag aaaaatagca gcaaaattca atattttatc aaattttgtt
      661 tatggtaatg agaaagaaaa catacaatat ttttctaaga agaagaaaaa tgaccagaat
      721 tttaagattt ttctcgaata tacgaaaaat tgataaacta aaattttcga tttttccatt
      781 tattttgaaa aaaaaactct tgacggtttt caccatgcga agaggtgaga aatattctga
      841 aagttatgca ttttgaaaaa attgcaaaaa aatacgttaa aattttttga tctattgttt
      901 ttttttgaaa accggaattt aaaatggaat tttttttcaa aaattaaaat taaaatttaa
      961 atatttctag tgtttggata gtttttcaaa agctagattt aacccgcgag atgtcgaccg
     1021 ctagtgtagc ttacagatgg ctgaagactt cgcaaaaatc ttatcttaga aacccgcgag
     1081 gtgtcggccg ctaaaacttt gtaaacctgc caacaaaatg cttcgcattg tctgaacaga
     1141 tgaaaccttg aaacaaaaca tatacaattg aaatcccgta aagtgtcggg cgcaagaact
     1201 gcgattagtt gtataattgg actagctcaa atttgaccaa aatctcattt ttagacctct
     1261 aagatgtcgg ccactataac tttattcaac ttgaaactgc aaaaaaactt atcaactgta
     1321 tagattcaag tattatgtca agtattggtt ttagagctac agtacactct gataacaaaa
     1381 tctgcagaaa tacctccctg tcaattccca aaatacctcc cctcatctca attatcccga
     1441 aaatcggtta gtcattgcca tcagaaaatc gaattaggta ccatgatggt agcaaactca
     1501 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1561 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1621 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1681 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1741 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1801 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1861 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1921 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     1981 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2041 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2101 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2161 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2221 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2281 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2341 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2401 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2461 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2521 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2581 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2641 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2701 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2761 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2821 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2881 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     2941 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3001 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3061 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3121 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3181 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3241 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3301 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3361 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3421 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3481 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3541 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3601 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3661 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3721 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3781 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3841 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3901 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     3961 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4021 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4081 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4141 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4201 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4261 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4321 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4381 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4441 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4501 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4561 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4621 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4681 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4741 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4801 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4861 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4921 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     4981 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5041 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5101 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5161 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5221 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5281 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5341 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5401 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5461 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5521 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5581 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5641 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5701 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5761 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5821 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5881 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5941 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6001 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6061 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6121 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6181 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6241 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6301 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6361 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6421 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6481 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6541 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6601 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6661 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6721 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6781 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6841 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6901 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     6961 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7021 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcggccg cagaaacagt
     7081 ggccgtattg ggaaaaatga acgatttttc ggcgggaata atttattttt ttatgttttt
     7141 ctatgcgttt tcgggtgttt tcgggttgct aagcgattgg ttgccttttt gaatcactag
     7201 tcttggtttt tgttgtttct gtgaatgaat aattggtttt tcgaggtttt tttgtcaaac
     7261 atgcctaaaa aataaattat gacgttttag ttgatttgtt tgttctttaa acgtctgaaa
     7321 ataaggttta aatctaattt attaattata aaattcgtca aaataagttg cgcgtcaaat
     7381 tatatgtatt gtacgcagtg tcaaactcca ggcctcagtt ttcatgaatt taccagcgat
     7441 ttttgttata aattttttta ttgaaattta aaatttttat ttttcaacca atttgcctcg
     7501 aaaattcgtt atttccccat taaaaaccgc ttttctaaag tgttgcgcgt caaataaaat
     7561 gcctggtacg caatgcacgg agaatgcgca aaggacgact gctggcgcac tttttgaatg
     7621 cggtaaattg aggcgcgaag tttcattcga aaacgcgcgc gaaacttcat tcatcgcact
     7681 ttctccgttc atttcgtcct atttttttgt ggtttttcgc gattttttcg cttttctgag
     7741 tgaaaaaata attttccttc gttttttcaa tgaaaatccg cggaaaaccc attttttccc
     7801 gtgaaaatcc gcatttttcg ctgtatttca taatttttat tcagatctcc cgtcaaaatg
     7861 tttgaggcca agctcgccaa cgccggactc cttaagaaga tcgtcgaatc gatcaaggat
     7921 ctcgtcaccg atgctccatt cgattgctcc gagacggcga tgagccttca ggctatggac
     7981 tcgtctcacg tcgcgttggt ttcgctcaag ttggaggtaa aattgggaaa aatgaagaaa
     8041 attagctaat tttccgtgtt caggtcggcc ttttcgacac atatcgctgc gatcggacca
     8101 tcaacctcgg actttcgttg gctaatatgt cgaaggcgct caagtgcgcc aacaacgacg
     8161 atacctgcat gctgaaggtg attgtaaaag ctttaaatcg aagaaatcag catttttcac
     8221 cttgaattgt ggattttcat gcgaaaaatc gcttcattcg gactttttcg cccgaaaaat
     8281 ccgaaaatgt ggtttttcgg tagtcaaaag tggcaaaatc agtttttttt ttcgcactaa
     8341 aaatcattga aaattaatat ttttagagac ttttaggccg aaatagtctg aattaatcat
     8401 ttttcgttct aaaaattatt agaaattcac atttttggcg gaaatcagca tttttcaccc
     8461 gaaaaatcac tggaaactca aattgttatc gatttttatg ccaaaatagt ccgagttttc
     8521 cattttatgc cttaaaaatc attagaaaac aacatttccg cctgaaaaat cattgaaaat
     8581 tcaaattttt tatcgatttt tccccgaaaa attccaattt taagcccaaa aattaccaat
     8641 tttcattgca gtatgaggaa aacgaaggcg actcgatcat cttcacattc gccgatccaa
     8701 agcgtgacaa gacccaagac gtgacagtca aaatgatgga cattgacagc gaacatctcg
     8761 ggatcccgga tcaagactac gccgtcgttt gtgagatgcc tgccggcgag ttccagaaga
     8821 cttgcaagga tctgtcgacg ttctccgact cgttgaacat caccgccacc aaggctggca
     8881 tcgtgttcac cggaaaggga gacatcggat cttctgtcgt cacctactcc ccatcttcca
     8941 acactgacga tgagactgag gctgtaacac tggaggtcaa ggatccggtt aatgtgaact
     9001 tctcgatcaa gtacatgaat cagttcacca aggcgacggc actttccgac agagttcgtc
     9061 tctctctgtg caacgatgtt ccggtcgttg tcgagtatcc gatcgaggag aatggatatc
     9121 ttcgcttcta cttggctcca aagattgacg acgacgagaa tatggacgga gcatcgatga
     9181 gtaaaggaga agaattgttc actggagttg tcccaatcct cgtcgagctc gacggagacg
     9241 tcaacggaca caagttctcc gtctccggag agggagaggg agacgccacc tacggaaagc
     9301 tcaccctcaa gttcatctgc accaccggaa agctcccagt cccatggcca accctcgtca
     9361 ccaccttctg ctacggagtc caatgcttct cccgttaccc agaccacatg aagcgtcacg
     9421 acttcttcaa gtccgccatg ccagagggat acgtccaaga gcgtaccatc ttcttcaagg
     9481 taagtttaaa catatatata ctaactactg attatttaaa ttttcaggac gacggaaact
     9541 acaagacccg tgccgaggtc aagttcgagg gagacaccct cgtcaaccgt atcgagctca
     9601 aggtaagttt aaacagttcg gtactaacta accatacata tttaaatttt cagggaatcg
     9661 acttcaagga ggacggaaac atcctcggac acaagctcga gtacaactac aactcccaca
     9721 acgtctacat catggccgac aagcaaaaga acggaatcaa ggtcaacttc aaggtaagtt
     9781 taaacatgat tttactaact aactaatctg atttaaattt tcagatccgt cacaacatcg
     9841 aggacggatc cgtccaactc gccgaccact accaacaaaa caccccaatc ggagacggac
     9901 cagtcctcct cccagacaac cactacctct ccacccaatc cgccctctcc aaggacccaa
     9961 acgagaagcg tgaccacatg gtcctcctcg agttcgtcac cgccgccgga atcacccacg
    10021 gaatggacga gctctacaag tcaggagcta gcggagccta cccttacgac gtgccagatt
    10081 acgcttaccc ctacgacgta ccagactacg cctacccata cgacgtccca gactacgctt
    10141 aagataaatg caaaatcctt tcaagcattc ccttcttctc tatcactctt ctttcttttt
    10201 gtcaaaaaat tctctcgcta atttatttgc ttttttaatg ttattatttt atgacttttt
    10261 atagtcactg aaaagtttgc atctgagtga agtgaatgct atcaaaatgt gattctgtct
    10321 gatgtacttt cacaatctct cttcaattcc attttgaagt gctttaaacc cgaaaggttg
    10381 agaaaaatgc gagcgctcaa atatttgtat tgtgttcgtt gagtgaccca acaaaaagag
    10441 gaaactttat tgtgccgcca agaaaaaagt ctcacgtacg gtgcgcgcga tgccctaggc
    10501 atggtcatag ctgtttcctg cgcaagtcgg cgatttcttt gaagttttgt caaaagaaga
    10561 gacaaggact tgatagatac gctcatcaga gtgagattca ttgcaaatag tgtgtaaatc
    10621 gtggagagac gagagaaaag gtgtgagaag cttagaagat aaagaatagg aagaagaaca
    10681 tatctcattt cctattttct ctaatttttc gttcattttt ctagacatta cccatttgat
    10741 ggtttttcaa tatggttatc agggtactca ttttggtagt tttcaaagca atttggagtt
    10801 tttttttaaa ttgtcaacta caaaaacgca gcttaaaggt ccaaatatca tagtataaaa
    10861 attctttaaa aaattccaaa ttttatatga ttttttgaaa attggaagaa tgttagtttt
    10921 tgccattttt ttggaagttt ttctccggat tttatggtac ttatatttca aatataaatt
    10981 tttgtaggaa ttcgagtatg cgagagagct atagtttact aaaaaaaagt ggcaaaaact
    11041 cagtaattgc cacttttttc aaaaatgttt tgaaaaattt tactatgata tttggccatt
    11101 ttggcaccat atgtacgtta ctttttacga aacactctaa tttttgaaat ttgagtttca
    11161 tgtttaatct tttgtataaa atttttatgg tccttttgtg acaggataaa aaattgcaaa
    11221 aaaatagaaa actttgtttc gtgaaaacac ctagtttaaa aattgtgggc cacttagcga
    11281 ttctagtcaa aaatcagaca aatgtcagct gccgtaactt tttccggatc agggttatca
    11341 aattttaatt ttaattgtaa atgtagaaaa atgatcggca acattttgct aattgaaaat
    11401 attttgaaaa ttgctatagt acatattaat ctagtattaa tatagtatta atgttataat
    11461 atcagttatg tgtttgaata actcaagcgt aagtttccgt aaaatatctt taaattttgg
    11521 taatttttta cggtttggta taaatttttg cattttttga ttttctacac tttttttcga
    11581 tttcgcattt tccgaatttc tctgaaataa atcgaacaat cggaaaattt tcgagggcac
    11641 ccttagccat gcagctcagg aatttgactt tatgttccca ttcaaatttt tttaaaatta
    11701 aataaaatgc taattgtaag ataaattaca aaaattatta aatctcatca tccttatgat
    11761 gatagtgcag tgcatgaata tatttttcca aataggatat gattcgtcga gtagcttcac
    11821 gtaatccgtt ttcttcaatc accatttcga actgcaattt tttttttgga tttttgtaac
    11881 aatattgtca taaattgtga ttttgatatt ttgcgtagag catcagaacc tttctgcttt
    11941 gaattttaat gggattaaaa attataaaaa acttgcaccc taattaaagc gccaacttta
    12001 ttatacatag ttgataattc gatggtatca gctcactcaa aggcggtaat acggttatcc
    12061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaagaccagg
    12121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    12181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    12241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    12301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    12361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    12541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    12601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12781 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    12841 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    12901 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    12961 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    13021 tctgacccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    13081 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13141 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13201 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    13261 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    13321 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    13381 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    13441 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    13501 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    13561 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    13621 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    13681 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    13741 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    13801 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    13861 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
    13921 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
    13981 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
    14041 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
    14101 cggggctggc ttaactatgc ggcatcagag cagattgcac tgagagtgca ccatatgcgg
    14161 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc
    14221 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    14281 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggat ccgccaagct
    14341 atcaactttg tatagaaaag ttg
//
