LOCUS       sequence-324062-        7486 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV rep/cap plasmid. Capsid is mouse brain tropic AAV-F.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7486)
  AUTHORS   Hanlon KS, Meltzer JC, Buzhdygan T, Cheng MJ, Sena-Esteves M, 
            Bennett RE, Sullivan TP, Razmpour R, Gong Y, Ng C, Nammour J, Maiz 
            D, Dujardin S, Ramirez SH, Hudry E, Maguire CA
  TITLE     Selection of an Efficient AAV Vector for Robust CNS Transgene 
            Expression.
  JOURNAL   Mol Ther Methods Clin Dev. 2019 Oct 23;15:320-332. doi: 
            10.1016/j.omtm.2019.10.007. eCollection 2019 Dec 13.
  PUBMED    31788496
REFERENCE   2  (bases 1 to 7486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2019-10-23"; volume: "15:320-332. doi"; 
            pages: " 10.1016/j.omtm.2019.10.007. eCollection 2019 Dec 13"
COMMENT     Sequence Label: AAV-F in pAR-9
FEATURES             Location/Qualifiers
     source          1..7486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3956..3967
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(4192..4210)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4231..4247)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4231..4247)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4244..4266)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4255..4271
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4279..4285,4286..4303,4304..4309))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4324..4345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4462..4479)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4633..5221)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4713..4732)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5392..6183,6184..6252))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6015..6034
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6253..6357)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6425..6443
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6481..6503)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6603..6622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6689..7144)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6826..6847)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7038..7057
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7274..7296
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7288..7305
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7289..7305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7312..7331
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7312..7330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
       61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
      121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
      181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg
      241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg
      301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt
      361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc
      421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa
      481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg
      541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag
      601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact
      661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag
      721 cagtggatcc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg
      781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc
      841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa
      901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc
      961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag
     1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc
     1081 aatgagaact ttcccttcaa cgactgtgtc gacaagatgg tgatctggtg ggaggagggg
     1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc
     1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc
     1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg
     1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag
     1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg
     1441 gagcatgaat tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca
     1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg
     1561 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg
     1621 aatctgatgc tgtttccctg cagacaatgc gagagaatga atcagaattc aaatatctgc
     1681 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt
     1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg
     1801 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa
     1861 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga
     1921 caaccttagt gaaggaattc gcgagtggtg ggctttgaaa cctggagccc ctcaacccaa
     1981 ggcaaatcaa caacatcaag acaacgctcg aggtcttgtg cttccgggtt acaaatacct
     2041 tggacccggc aacggactcg acaaggggga gccggtcaac gcagcagacg cggcggccct
     2101 cgagcacgac aaggcctacg accagcagct caaggccgga gacaacccgt acctcaagta
     2161 caaccacgcc gacgccgagt tccaggagcg gctcaaagaa gatacgtctt ttgggggcaa
     2221 cctcgggcga gcagtcttcc aggccaaaaa gaggcttctt gaacctcttg gtctggttga
     2281 ggaagcggct aagacggctc ctggaaagaa gaggcctgta gagcagtctc ctcaggaacc
     2341 ggactcctcc gcgggtattg gcaaatcggg tgcacagccc gctaaaaaga gactcaattt
     2401 cggtcagact ggcgacacag agtcagtccc agaccctcaa ccaatcggag aacctcccgc
     2461 agccccctca ggtgtgggat ctcttacaat ggcttcaggt ggtggcgcac cagtggcaga
     2521 caataacgaa ggtgccgatg gagtgggtag ttcctcggga aattggcatt gcgattccca
     2581 atggctgggg gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa
     2641 caatcacctc tacaagcaaa tctccaacag cacatctgga ggatcttcaa atgacaacgc
     2701 ctacttcggc tacagcaccc cctgggggta ttttgacttc aacagattcc actgccactt
     2761 ctcaccacgt gactggcagc gactcatcaa caacaactgg ggattccggc ctaagcgact
     2821 caacttcaag ctcttcaaca ttcaggtcaa agaggttacg gacaacaatg gagtcaagac
     2881 catcgccaat aaccttacca gcacggtcca ggtcttcacg gactcagact atcagctccc
     2941 gtacgtgctc gggtcggctc acgagggctg cctcccgccg ttcccagcgg acgttttcat
     3001 gattcctcag tacgggtatc tgacgcttaa tgatggaagc caggccgtgg gtcgttcgtc
     3061 cttttactgc ctggaatatt tcccgtcgca aatgctaaga acgggtaaca acttccagtt
     3121 cagctacgag tttgagaacg tacctttcca tagcagctac gctcacagcc aaagcctgga
     3181 ccgactaatg aatccactca tcgaccaata cttgtactat ctctcaaaga ctattaacgg
     3241 ttctggacag aatcaacaaa cgctaaaatt cagtgtggcc ggacccagca acatggctgt
     3301 ccagggaaga aactacatac ctggacccag ctaccgacaa caacgtgtct caaccactgt
     3361 gactcaaaac aacaacagcg aatttgcttg gcctggagct tcttcttggg ctctcaatgg
     3421 acgtaatagc ttgatgaatc ctggacctgc tatggccagc cacaaagaag gagaggaccg
     3481 tttctttcct ttgtctggat ctttaatttt tggcaaacaa ggaactggaa gagacaacgt
     3541 ggatgcggac aaagtcatga taaccaacga agaagaaatt aaaactacta acccggtagc
     3601 aacggagtcc tatggacaag tggccacaaa ccaccagagt gcccaatttg ttgttggtca
     3661 gagttatgca caggcgcaga ccggctgggt tcaaaaccaa ggaatacttc cgggtatggt
     3721 ttggcaggac agagatgtgt acctgcaagg acccatttgg gccaaaattc ctcacacgga
     3781 cggcaacttt cacccttctc cgctgatggg agggtttgga atgaagcacc cgcctcctca
     3841 gatcctcatc aaaaacacac ctgtacctgc ggatcctcca acggccttca acaaggacaa
     3901 gctgaactct ttcatcaccc agtattctac tggccaagtc agcgtggaga tcgagtggga
     3961 gctgcagaag gaaaacagca agcgctggaa cccggagatc cagtacactt ccaactatta
     4021 caagtctaat aatgttgaat ttgctgttaa tactgaaggt gtatatagtg aaccccgccc
     4081 cattggcacc agatacctga ctcgtaatct gtaattacgt gttaatcaat aaaccggttg
     4141 attcgtttca gttgaacttt ggtgtcgcgg ccgctcgata agcttttgtt ccctttagtg
     4201 agggttaatt ccgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4261 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4321 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4381 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4441 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     4501 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     4561 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4621 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4681 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4741 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4801 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4861 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4921 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4981 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5041 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     5101 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5161 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5221 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     5281 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     5341 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     5401 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     5461 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     5521 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     5581 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     5641 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     5701 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     5761 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     5821 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     5881 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     5941 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     6001 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     6061 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     6121 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     6181 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     6241 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     6301 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     6361 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     6421 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     6481 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     6541 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
     6601 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     6661 atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt gttaaaattc
     6721 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     6781 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6841 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     6901 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6961 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     7021 aacgtggcga ggaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     7081 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     7141 gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     7201 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     7261 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     7321 tcactatagg gcgaattcga gctcggtacc cctagagtcc tgtattagag gtcacgtgag
     7381 tgttttgcga cattttgcga caccatgtgg tcacgctggg tatttaagcc cgagtgagca
     7441 cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgcc
//
