LOCUS       sequence-324064-       15631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rps-27>DHB::2xmKate2-P2A-H2B::GFP expression plasmid for CRISPR/Cas9
            integration at ttTi4348 site on chromosome I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15631)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 15631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pWZ194
FEATURES             Location/Qualifiers
     source          1..15631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1507..1540)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     CDS             complement(1540..1551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      complement(2973..3296)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    5214..5238
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5241..6266
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5241..6266
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(6267..6291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6644..6677)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     primer_bind     7046..7063
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7047..7063
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             10019..10075
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11327..11407
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      11416..11739
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     primer_bind     complement(11772..11788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11772..11788)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(13261..13281)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     rep_origin      complement(13418..14006)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13498..13517)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14177..14968,14969..15037))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14800..14819
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15038..15142)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     15210..15228
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(15266..15288)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    15611..15631
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 caagtgggga tcaggaagaa gttgataaag tgattcaaaa actcgaggag ctcaacacag
       61 cgatgaaccc aattttgaga gcaacactga cgaagaacta tgacgcgagg gtaggcacta
      121 ccttgaagct atccacctgc ctgcctgccg cctatttcca tgtgcctact gcgcatggaa
      181 ataggcggta gtcaggcagg tagatagcct cataaaattc aatacttcct tttcagaaag
      241 aatgctggaa tgtattcaaa gaggagtcac tcttcccatt gctcagagaa tatgaagagt
      301 tgctcggcga gcaaaagttc atgattggag atcgatattc atgggctgac atcgctgtcg
      361 tcgagtttct aactcggtgt cagcagtgct acgatagctt ctatctggct catttcgaga
      421 ggctccgaac tttttgccag acttttgagt ctcttccgca cattcgtccc tacattcagg
      481 gacgtgttga ttcatttatc taatttttta aaatggatat ttttcgtttt ttatttgttt
      541 gttttgctga taattcaata aagttacgca acgaatttta gacatttatg aaaaccgaaa
      601 atcgacaaaa atattttaag aaaaatagca gcaaaattca atattttatc aaattttgtt
      661 tatggtaatg agaaagaaaa catacaatat ttttctaaga agaagaaaaa tgaccagaat
      721 tttaagattt ttctcgaata tacgaaaaat tgataaacta aaattttcga tttttccatt
      781 tattttgaaa aaaaaactct tgacggtttt caccatgcga agaggtgaga aatattctga
      841 aagttatgca ttttgaaaaa attgcaaaaa aatacgttaa aattttttga tctattgttt
      901 ttttttgaaa accggaattt aaaatggaat tttttttcaa aaattaaaat taaaatttaa
      961 atatttctag tgtttggata gtttttcaaa agctagattt aacccgcgag atgtcgaccg
     1021 ctagtgtagc ttacagatgg ctgaagactt cgcaaaaatc ttatcttaga aacccgcgag
     1081 gtgtcggccg ctaaaacttt gtaaacctgc caacaaaatg cttcgcattg tctgaacaga
     1141 tgaaaccttg aaacaaaaca tatacaattg aaatcccgta aagtgtcggg cgcaagaact
     1201 gcgattagtt gtataattgg actagctcaa atttgaccaa aatctcattt ttagacctct
     1261 aagatgtcgg ccactataac tttattcaac ttgaaactgc aaaaaaactt atcaactgta
     1321 tagattcaag tattatgtca agtattggtt ttagagctac agtacactct gataacaaaa
     1381 tctgcagaaa tacctccctg tcaattccca aaatacctcc cctcatctca attatcccga
     1441 aaatcggtta gtcattgcca tcagaaaatc gaattaggta ccatgatggt agcaaactca
     1501 cttcgtataa cttcgtatag tataccttat acgaagttat ctgccctcga ttgtctatct
     1561 tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt catttcccaa
     1621 ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc tacatccgta
     1681 ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca cactgtctcg
     1741 tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat ttcctaataa
     1801 aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact aggtgagtta
     1861 catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag atgtctgtaa
     1921 aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta tgcttcatga
     1981 ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat accgagatga
     2041 atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa ttttctagca
     2101 atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca agcgtgtccg
     2161 ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat ccccatctgc
     2221 tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga ctccaaatgg
     2281 agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc cagctggacc
     2341 atccggacca aagggagttc caggagttcc aggactcgac ggagttccag gacttgacgg
     2401 tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg gatgcttcac
     2461 ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac cacgtggact
     2521 tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag gacatccagg
     2581 agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac caggagagaa
     2641 gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa gaggagatca
     2701 aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag agccaggaac
     2761 cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg gaacccaggg
     2821 agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt gcccagacaa
     2881 gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct aaattgttgg
     2941 tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc acaataaagt
     3001 ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg ctcgcatttt
     3061 tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt gtgaaagtac
     3121 atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact tttcagtgac
     3181 tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag agaatttttt
     3241 gacaaaaaga aagaatagtg atagagaaga agggaatgct tgaaaggatt ttgcatttat
     3301 cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc aagtttctaa
     3361 tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc tctgggatgg
     3421 aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt ccagaccaag
     3481 ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg gtagcttcga
     3541 agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta aacttacctt
     3601 caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg ttcttgcgaa
     3661 cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa atccagcgct
     3721 caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc gagaccagcg
     3781 tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta atatccttga
     3841 cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt ccgaggaagg
     3901 cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg acctgatcga
     3961 agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt taaacttacc
     4021 tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc ttgcggatac
     4081 gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca gatcggcgat
     4141 ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat ccacgagctt
     4201 ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac ttgcggttgt
     4261 taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc caggtgtgct
     4321 cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact tcatcacttg
     4381 tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt gacatatttt
     4441 cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc cgctttcctt
     4501 atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg cattcgtttg
     4561 aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg tctctttatt
     4621 gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa caagcagctc
     4681 gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa taccgaacaa
     4741 catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac tttaccacta
     4801 tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc gtaaatctac
     4861 acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga ctgagtattc
     4921 aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat gttctttttt
     4981 cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt tcaattaaaa
     5041 aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat tttcttattc
     5101 agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt ttcttggtta
     5161 tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt gccgcaagtt
     5221 tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     5281 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     5341 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     5401 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     5461 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     5521 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     5581 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     5641 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     5701 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     5761 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     5821 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     5881 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     5941 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     6001 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     6061 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     6121 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     6181 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     6241 gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg gtccaattac
     6301 tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat
     6361 caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga
     6421 aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc
     6481 cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct
     6541 tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat
     6601 accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt cgtatagtat
     6661 accttatacg aagttatact agtatatatt tttatgtttt cttttaaacc ttcctccata
     6721 tttctgaatt gaatgttgtt gcatattgtg tgtccttatt tcatttccat catccttttg
     6781 tgtgtttccc ccttccacca attcccccca cgtcctaatg aaatgaaagc gagaagataa
     6841 ataaacttgc acattgtgtt cagatgagag gagcggaata cgaattggga gacggaaaga
     6901 gaaaaaaaaa tgtttagatt tggattgaat taatttttta gaaagcaaga aaaaaaacac
     6961 aaagagaata aataaataat aggaaaaaaa acaaatattc agtatttaaa atttgaaaat
     7021 tcaacgacgt tggcgtcgat catcctgtaa aacgacggcc agtgcggccg cttcaatcgg
     7081 tttttccttg cttgcgccca attctgatgg ttcttccatt tctaaatttt agtttaataa
     7141 aaaaatttta aaataatacg agttttgaga ctaactttca ataaaaacca aaaaaaattg
     7201 cttcaaaaaa gagttcgcgc cgatttacga taaacacagc gcgctacgac actccgaaaa
     7261 ccgcgccgca ccattacatt ctccgcccca ccgcacggtt tgcggaaggc gatgaaattg
     7321 ctgggcctaa aattttaacc ctttttttcc cgtttttcaa ctgttttccc gattttttcg
     7381 atattttttt caggttttta gttagaaaag tatataaaaa ttgcattatt tgtagttttc
     7441 gctcgtaatt tgtgatagaa aacggccaat ttgcctagat ttgtgattct accaagtgga
     7501 atttttggga aaatatcaat attccacgat ttttattcaa atcataacgt ttcgaaatta
     7561 tcagcttaaa taaaactaaa ataaataata aaatactaaa aaccctgaat ttctcggttc
     7621 tcggttcagc ttgagttcgt tctatacacg ttatcgattt ttgtcatttc gtttaatcat
     7681 gggttttgat tcattttaaa tatgaaattt gatttattcc tcgtttcgaa gttggtttgt
     7741 cttgtccggc cggtaaacaa atgttattaa aaaaataata aatttcgaaa tttttcgtaa
     7801 tgttccgcaa tcgtgttttt ccgtttatct cgtcttccct tctttcaaat atactattta
     7861 ttttctcaat tcacatattt tcagccagcc ccgctcaaca agtggaataa aaatgaccaa
     7921 cgacgtcacc tggtccgagg cctcctcccc agacgagcgt accctcacct tcgccgagcg
     7981 ttggcaactc tcctccccag acggagtcga caccgacgac gacctcccaa agtcccgtgc
     8041 ctccaagcgt acctgcggag tcaacgacga cgagtcccca tccaaggtaa gtttaaacat
     8101 atatatacta actaaccctg attatttaaa ttttcagatc ttcatggtcg gagagtcccc
     8161 acaagtctcc tcccgtctcc aaaacctccg tctcaacaac ctcatcccac gtcaactctt
     8221 caagccaacc gacaaccaag agaccggagc atcgggagcc tcaggagcat cgatggtctc
     8281 cgagctcatt aaagaaaaca tgcatatgaa gctctacatg gagggaaccg tcaacaacca
     8341 ccacttcaag tgcacctccg agggagaggg aaagccatac gagggaaccc aaaccatgcg
     8401 tatcaaggcc gtcgagggag gaccactccc attcgccttc gacatcctcg ccacctcctt
     8461 catgtacgga tccaaggtaa gtttaaacat atatatacta actaaccctg attatttaaa
     8521 ttttcagacc ttcatcaacc acacccaagg aatcccagac ttcttcaagc aatccttccc
     8581 agagggattc acctgggagc gtgtcaccac ctacgaggac ggaggagtcc tcaccgccac
     8641 ccaagacacc tccctccaag acggatgcct catctacaac gtcaaggtaa gtttaaacag
     8701 ttcggtacta actaaccata catatttaaa ttttcagatc cgtggagtca acttcccatc
     8761 caacggacca gtcatgcaaa agaagaccct cggatgggag gcctccaccg agaccctcta
     8821 cccagccgac ggaggacttg agggacgtgc cgacatggcc ctcaagctcg tcggaggagg
     8881 acacctcatc tgcaacctca aggtaagttt aaacatgatt ttactaacta actaatctga
     8941 tttaaatttt cagaccacct accgttccaa gaagccagcc aagaacctca agatgccagg
     9001 agtctactac gtcgaccgtc gtcttgagcg tatcaaggag gccgacaagg agacctacgt
     9061 cgagcaacac gaggtcgccg tcgcccgtta ctgcgacctc ccatccaagc tcggacaccg
     9121 tggtggagga ggatctggag gaggaggatc catggtttcc gagttgatca aggagaatat
     9181 gcacatgaaa ctctacatgg aaggaaccgt caacaatcat catttcaaat gtacttcaga
     9241 aggagaagga aaaccatacg agggaactca gaccatgaga atcaaggctg ttgagggagg
     9301 taagtttaaa aaatacgtgt tcataacttg atacgaatat aaattttcag gaccacttcc
     9361 attcgcgttc gacattcttg ccacttcgtt catgtatgga agcaagactt tcattaatca
     9421 cacacaaggg atcccggatt tcttcaagca atctttccca gaaggattca catgggagag
     9481 agttaccact tacgaggatg gaggagttct taccgccacc caagacacat ccctccaagg
     9541 taagtttaaa agattatagc aatgttcgtc taaatataat aaattttcag atggatgtct
     9601 gatctacaac gtcaagatca gaggagtcaa tttcccatca aatggaccag ttatgcagaa
     9661 gaagactttg ggctgggagg cttccaccga gaccctttac ccagcagatg gaggtctcga
     9721 gggccgcgct gatatggctc tcaaactcgt cggaggagga cacttgatct gcaatttaaa
     9781 aaccacctac cggtcgaagg taagtttaaa atcaattttt aatagcttat ataataatac
     9841 aaattttcag aagccagcca agaatctcaa aatgccagga gtttactatg ttgaccgtcg
     9901 tcttgagcgc attaaagagg ccgataagga aacgtacgtt gaacaacatg aagttgccgt
     9961 tgctcgatat tgtgatctcc catccaagct cggacatcgt gccggcgcgg gaagtggggc
    10021 cacgaacttc agtctcctca aacaagccgg ggacgtcgaa gagaaccccg ggccaatgcc
    10081 accaaagcca tctgccaagg gagccaagaa ggccgccaag accgtcgttg ccaagccaaa
    10141 ggacggaaag aagagacgtc atgcccgcaa ggaatcgtac tccgtctaca tctaccgtgt
    10201 tctcaagcaa gttcacccag acaccggagt ctcctccaag gccatgtcta tcatgaactc
    10261 cttcgtcaac gatgtattcg aacgcatcgc ttcggaagct tcccgtcttg ctcattacaa
    10321 caaacgctca acgatctcat cccgcgaaat tcaaaccgct gtccgtttga ttctcccagg
    10381 agaacttgcc aagcacgccg tgtctgaggg aaccaaggcc gtcaccaagt acacttccag
    10441 caagatgagt aaaggagaag aattgttcac tggagttgtc ccaatcctcg tcgagctcga
    10501 cggagacgtc aacggacaca agttctccgt ctccggagag ggagagggag acgccaccta
    10561 cggaaagctc accctcaagt tcatctgcac caccggaaag ctcccagtcc catggccaac
    10621 cctcgtcacc accttctgct acggagtcca atgcttctcc cgttacccag accacatgaa
    10681 gcgtcacgac ttcttcaagt ccgccatgcc agagggatac gtccaagagc gtaccatctt
    10741 ctttaaggta agtttaaaca tatatatact aactactgat tatttaaatt ttcaggacga
    10801 cggaaactac aagacccgtg ccgaggtcaa gttcgaggga gacaccctcg tcaaccgtat
    10861 cgagctccag gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
    10921 gggaatcgac ttcaaggagg acggaaacat cctcggacac aagctcgagt acaactacaa
    10981 ctcccacaac gtctacatca tggccgacaa gcaaaagaac ggaatcaagg tcaacttcaa
    11041 ggtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc agatccgtca
    11101 caacatcgag gacggatccg tccaactcgc cgaccactac caacaaaaca ccccaatcgg
    11161 agacggacca gtcctcctcc cagacaacca ctacctctcc acccaatccg ccctctccaa
    11221 ggacccaaac gagaagcgtg accacatggt cctcctcgag ttcgtcaccg ccgccggaat
    11281 cacccacgga atggacgagc tctacaagtc aggagctagc ggagcctacc cttacgacgt
    11341 gccagattac gcttacccct acgacgtacc agactacgcc tacccatacg acgtcccaga
    11401 ctacgcttaa gataaatgca aaatcctttc aagcattccc ttcttctcta tcactcttct
    11461 ttctttttgt caaaaaattc tgtcgctaat ttatttgctt ttttaatgtt attattttat
    11521 gactttttat agtcactgaa aagtttgcat ctgagtgaag tgaatgctat caaaatgtga
    11581 ttctgtctga tgtactttca caatctctct tcaattccat tttgaagtgc tttaaacccg
    11641 aaaggttgag aaaaatgcga gcgctcaaat atttgtattg tgttcgttga gtgacccaac
    11701 aaaaagagga aactttattg tgccgccaag aaaaaagtct cacgtacggt gcgcgcgatg
    11761 ccctaggcat ggtcatagct gtttcctgcg caagtcggcg atttctttga agttttgtca
    11821 aaagaagaga caaggacttg atagatacgc tcatcagagt gagattcatt gcaaatagtg
    11881 tgtaaatcgt ggagagacga gagaaaaggt gtgagaagct tagaagataa agaataggaa
    11941 gaagaacata tctcatttcc tattttctct aatttttcgt tcatttttct agacattacc
    12001 catttgatgg tttttcaata tggttatcag ggtactcatt ttggtagttt tcaaagcaat
    12061 ttggagtttt tttttaaatt gtcaactaca aaaacgcagc ttaaaggtcc aaatatcata
    12121 gtataaaaat tctttaaaaa attccaaatt ttatatgatt ttttgaaaat tggaagaatg
    12181 ttagtttttg ccattttttt ggaagttttt ctccggattt tatggtactt atatttcaaa
    12241 tataaatttt tgtaggaatt cgagtatgcg agagagctat agtttactaa aaaaaagtgg
    12301 caaaaactca gtaattgcca cttttttcaa aaatgttttg aaaaatttta ctatgatatt
    12361 tggccatttt ggcaccatat gtacgttact ttttacgaaa cactctaatt tttgaaattt
    12421 gagtttcatg tttaatcttt tgtataaaat ttttatggtc cttttgtgac aggataaaaa
    12481 attgcaaaaa aatagaaaac tttgtttcgt gaaaacacct agtttaaaaa ttgtgggcca
    12541 cttagcgatt ctagtcaaaa atcagacaaa tgtcagctgc cgtaactttt tccggatcag
    12601 ggttatcaaa ttttaatttt aattgtaaat gtagaaaaat gatcggcaac attttgctaa
    12661 ttgaaaatat tttgaaaatt gctatagtac atattaatct agtattaata tagtattaat
    12721 gttataatat cagttatgtg tttgaataac tcaagcgtaa gtttccgtaa aatatcttta
    12781 aattttggta attttttacg gtttggtata aatttttgca ttttttgatt ttctacactt
    12841 tttttcgatt tcgcattttc cgaatttctc tgaaataaat cgaacaatcg gaaaattttc
    12901 gagggcaccc ttagccatgc agctcaggaa tttgacttta tgttcccatt caaatttttt
    12961 taaaattaaa taaaatgcta attgtaagat aaattacaaa aattattaaa tctcatcatc
    13021 cttatgatga tagtgcagtg catgaatata tttttccaaa taggatatga ttcgtcgagt
    13081 agcttcacgt aatccgtttt cttcaatcac catttcgaac tgcaattttt tttttggatt
    13141 tttgtaacaa tattgtcata aattgtgatt ttgatatttt gcgtagagca tcagaacctt
    13201 tctgctttga attttaatgg gattaaaaat tataaaaaac ttgcacccta attaaagcgc
    13261 caactttatt atacatagtt gataattcga tggtatcagc tcactcaaag gcggtaatac
    13321 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    13381 agaccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    13441 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    13501 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    13561 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    13621 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    13681 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    13741 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    13801 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    13861 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    13921 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    13981 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    14041 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    14101 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    14161 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    14221 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    14281 gcttaccatc tgaccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    14341 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    14401 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    14461 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    14521 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    14581 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    14641 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    14701 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    14761 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    14821 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    14881 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    14941 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    15001 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    15061 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    15121 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    15181 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
    15241 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
    15301 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
    15361 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgcactg agagtgcacc
    15421 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
    15481 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
    15541 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggatcc
    15601 gccaagctat caactttgta tagaaaagtt g
//
