LOCUS       sequence-324066-        5513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing soluble CD200 in retinal pigment epithelium.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Wang SK, Xue Y, Rana P, Hong CM, Cepko CL
  TITLE     Soluble CX3CL1 gene therapy improves cone survival and function in 
            mouse models of retinitis pigmentosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 May 14;116(20):10140-10149. doi: 
            10.1073/pnas.1901787116. Epub 2019 Apr 29.
  PUBMED    31036641
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1901787116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-05-14- 14"; volume: "116"; issue: "20"; pages: 
            "10140-10149"
COMMENT     Sequence Label: AAV-Best1-sCD200
FEATURES             Location/Qualifiers
     source          1..5513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     intron          829..961
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     944..963
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      981..990
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1769..1789)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2187..2198)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2265..2282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2271..2478
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   2566..2695
                     /label=AAV2 ITR
     primer_bind     complement(2714..2731)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2714..2730)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2723..2745)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2872..3327
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2959..2978)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3169..3190
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3353..3457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3458..3526,3527..4318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3676..3695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4489..5077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4978..4997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5365..5386
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5401..5406,5407..5424,5425..5431)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5439..5455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5444..5466
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5463..5479
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5463..5479
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aggctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggaaga tcggaattcg cccttaagct agctcctttt cagataaggg cactgaggct
      241 gagagaggag ctgaaaccta cccggggtca ccacacacag gtggcaaggc tgggaccaga
      301 aaccaggact gttgactgca gcccggtatt cattctttcc atagcccaca gggctgtcaa
      361 agaccccagg gcctagtcag aggctcctcc ttcctggaga gttcctggca cagaagttga
      421 agctcagcac agccccctaa cccccaactc tctctgcaag gcctcagggg tcagaacact
      481 ggtggagcag atcctttagc ctctggattt tagggccatg gtagaggggg tgttgcccta
      541 aattccagcc ctggtctcag cccaacaccc tccaagaaga aattagaggg gccatggcca
      601 ggctgtgcta gccgttgctt ctgagcagat tacaagaagg gactaagaca aggactcctt
      661 tgtggaggtc ctggcttagg gagtcaagtg acggcggctc agcactcacg tgggcagtgc
      721 cagcctctaa gagtgggcag gggcactggc cacagagtcc cagggagtcc caccagccta
      781 gtcgccagac ccagatcctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
      841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
      901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
      961 ggtgtccagg cggccgcgcc gccaccatgg gcagtctggt attcaggaga cctttctgcc
     1021 atctctccac ctacagcctg atttggggca tggcagcagt agcgctgagc acagctcaag
     1081 tggaagtggt gacccaggat gaaagaaagg cgctgcacac aactgcatcc ttacgatgtt
     1141 ctctaaaaac atcccaggaa cccttgattg tgacatggca gaaaaagaaa gccgtgagcc
     1201 cagaaaacat ggtcacctac agcaaaaccc atggggttgt aatccagcct gcctacaaag
     1261 acaggataaa tgtcacagag ctgggactct ggaactcaag catcaccttc tggaacacaa
     1321 cattggaaga tgagggctgc tacatgtgtc tcttcaacac gtttggttct cagaaggtct
     1381 caggaacagc ttgccttacc ctctatgtac agcccatagt acaccttcac tacaactatt
     1441 ttgaagacca cctaaacatc acttgctctg cgactgcccg tccagcccct gccatctcct
     1501 ggaagggtac tgggacagga attgagaata gtaccgagag tcacttccat tcaaatggga
     1561 ctacatctgt caccagcatc ctccgggtca aagaccccaa aactcaagtt gggaaggaag
     1621 tgatctgcca ggttttatac ctggggaatg tgattgacta caagcagagt ctggacaaag
     1681 gattttggtt ttcagttcca taaaagcttg gatccaatca acctctggat tacaaaattt
     1741 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     1801 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     1861 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     1921 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     1981 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     2041 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     2101 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     2161 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     2221 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgaga tctgcctcga ctgtgccttc
     2281 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     2341 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     2401 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     2461 tagcaggcat gctggggact cgagttaagg gcgaattccc gataaggatc ttcctagagc
     2521 atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga
     2581 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     2641 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta
     2701 attaacctaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     2761 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     2821 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     2881 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     2941 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3001 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3061 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3121 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3181 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3241 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     3301 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     3361 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     3421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     3481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     3541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     3601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     3661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     3721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     3781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     3841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     3901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     3961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     4321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     4381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     4741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     4801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     4861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     4921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     4981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5221 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     5281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     5341 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     5401 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     5461 cacaggaaac agctatgacc atgattacgc cagatttaat taaggcctta att
//
