LOCUS       sequence-324067-        6800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing mCherry in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Wang SK, Xue Y, Rana P, Hong CM, Cepko CL
  TITLE     Soluble CX3CL1 gene therapy improves cone survival and function in 
            mouse models of retinitis pigmentosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 May 14;116(20):10140-10149. doi: 
            10.1073/pnas.1901787116. Epub 2019 Apr 29.
  PUBMED    31036641
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1901787116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-05-14- 14"; volume: "116"; issue: "20"; pages: 
            "10140-10149"
COMMENT     Sequence Label: AAV-hRedOpsin-mCherry
FEATURES             Location/Qualifiers
     source          1..6800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    78..99
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(114..119,120..137,138..144)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    152..168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     157..179
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     176..192
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     176..192
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   230..359
                     /label=AAV2 ITR
     regulatory      2506..2515
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2512..3222
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2657..2675)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2925..2944
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3282..3302)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3700..3711)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3778..3795)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3784..3991
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4079..4208
                     /label=AAV2 ITR
     primer_bind     complement(4227..4244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4227..4243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4236..4258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4385..4840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4472..4491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4682..4703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4866..4970
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4971..5039,5040..5831)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5189..5208)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6002..6590
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6491..6510
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
       61 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
      121 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
      181 aacagctatg accatgatta cgccagattt aattaaggcc ttaattaggc tgcgcgctcg
      241 ctcgctcact gaggccgccc gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc
      301 ctcagtgagc gagcgagcgc gcagagaggg agtggccaac tccatcacta ggggttcctt
      361 gtagttaatg attaacccgc catgctactt atctacgtag ccatgctcta ggaagatcgg
      421 aattcgccct taagctagcc tacagcagcc agggtgagat tatgaggctg agctgagaat
      481 atcaagactg taccgagtag ggggccttgg caagtgtgga gagcccggca gctggggcag
      541 agggcggagt acggtgtgcg tttacggacc tcttcaaacg aggtaggaag gtcagaagtc
      601 aaaaagggaa caaatgatgt ttaaccacac aaaaatgaaa atccaatggt tggatatcca
      661 ttccaaatac acaaaggcaa cggataagtg atccgggcca ggcacagaag gccatgcacc
      721 cgtaggattg cactcagagc tcccaaatgc ataggaatag aagggtgggt gcaggaggct
      781 gaggggtggg gaaagggcat gggtgtttca tgaggacaga gcttccgttt catgcaatga
      841 aaagagtttg gagacggatg gtggtgactg gactatacac ttacacacgg tagcgatggt
      901 acactttgta ttatgtatat tttaccacga tctttttaaa gtgtcaaagg caaatggcca
      961 aatggttcct tgtcctatag ctgtagcagc catcggctgt tagtgacaaa gcccctgagt
     1021 caagatgaca gcagccccca taactcctaa tcggctctcc cgcgtggagt catttaggag
     1081 tagtcgcatt agagacaagt ccaacatcta atcttccacc ctggccaggg ccccagctgg
     1141 cagcgagggt gggagactcc gggcagagca gagggcgctg acattggggc ccggcctggc
     1201 ttgggtccct ctggcctttc cccaggggcc ctctttcctt ggggctttct tgggccgcca
     1261 ctgctcccgc tcctctcccc ccatcccacc ccctcacccc ctcgttcttc atatccttct
     1321 ctagtgctcc ctccactttc atccaccctt ctgcaagagt gtgggaccac aaatgagttt
     1381 tcacctggcc tggggacaca cgtgccccca caggtgctga gtgactttct aggacagtaa
     1441 tctgctttag gctaaaatgg gacttgatct tctgttagcc ctaatcatca attagcagag
     1501 ccggtgaagg tgcagaacct accgcctttc caggcctcct cccacctctg ccacctccac
     1561 tctccttcct gggatgtggg ggctggcaca cgtgtggccc agggcattgg tgggattgca
     1621 ctgagctggg tcattagcgt aatcctggac aagggcagac agggcgagcg gagggccagc
     1681 tccggggctc aggcaaggct gggggcttcc cccagacacc ccactcctcc tctgctggac
     1741 ccccacttca tagggcactt cgtgttctca aagggcttcc aaatagcatg gtggccttgg
     1801 atgcccaggg aagcctcaga gttgcttatc tccctctaga cagaagggga atctcggtca
     1861 agagggagag gtcgccctgt tcaaggccac ccagccagct catggcggta atgggacaag
     1921 gctggccagc catcccaccc tcagaaggga cccggtgggg caggtgatct cagaggaggc
     1981 tcacttctgg gtctcacatt cttggatccg gttccaggcc tcggccctaa atagtctccc
     2041 tgggctttca agagaaccac atgagaaagg aggattcggg ctctgagcag tttcaccacc
     2101 caccccccag tctgcaaatc ctgacccgtg ggtccacctg ccccaaaggc ggacgcagga
     2161 cagtagaagg gaacagagaa cacataaaca cagagagggc cacagcggct cccacagtca
     2221 ccgccacctt cctggcgggg atgggtgggg cgtctgagtt tggttcccag caaatccctc
     2281 tgagccgccc ttgcgggctc gcctcaggag caggggagca agaggtggga ggaggaggtc
     2341 taagtcccag gcccaattaa gagatcaggt agtgtagggt ttgggagctt ttaaggtgaa
     2401 gaggcccggg ctgatcccac aggccagtat aaagcgccgt gaccctcagg tgatgcgcca
     2461 gggccggctg ccgtcgggga cagggctttc catagcggcc gcgccgccac catggtgagc
     2521 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     2581 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     2641 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     2701 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     2761 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     2821 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     2881 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     2941 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     3001 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     3061 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     3121 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     3181 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaaccggtaa tcaacctctg
     3241 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3301 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3361 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3421 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3481 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3541 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3601 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3661 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3721 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg agatctgcct
     3781 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     3841 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     3901 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     3961 attgggaaga caatagcagg catgctgggg actcgagtta agggcgaatt cccgataagg
     4021 atcttcctag agcatggcta cgtagataag tagcatggcg ggttaatcat taactacaag
     4081 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4141 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4201 gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4261 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4321 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4381 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4441 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4501 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4561 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4621 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4681 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4741 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4801 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4861 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4921 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4981 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5041 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5101 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5161 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5221 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5281 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5341 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5401 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5461 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5521 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5581 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5641 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5701 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5761 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5821 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5881 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5941 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6001 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6061 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6121 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6181 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6241 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6301 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6361 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6421 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6481 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6541 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6601 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6661 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6721 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6781 tacgcaaacc gcctctcccc
//
