LOCUS       sequence-324070-        6926 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing full-length CD200 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Wang SK, Xue Y, Rana P, Hong CM, Cepko CL
  TITLE     Soluble CX3CL1 gene therapy improves cone survival and function in 
            mouse models of retinitis pigmentosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 May 14;116(20):10140-10149. doi: 
            10.1073/pnas.1901787116. Epub 2019 Apr 29.
  PUBMED    31036641
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1901787116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-05-14- 14"; volume: "116"; issue: "20"; pages: 
            "10140-10149"
COMMENT     Sequence Label: AAV-hRedOpsin-fCD200
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     regulatory      2280..2289
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3182..3202)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3600..3611)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3678..3695)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3684..3891
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3979..4108
                     /label=AAV2 ITR
     primer_bind     complement(4127..4144)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4127..4143)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4136..4158)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4285..4740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4372..4391)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4582..4603
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4766..4870
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4871..4939,4940..5731)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5089..5108)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5902..6490
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6391..6410
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6778..6799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6814..6819,6820..6837,6838..6844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6852..6868
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6857..6879
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6876..6892
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6876..6892
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aggctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggaaga tcggaattcg cccttaagct agcctacagc agccagggtg agattatgag
      241 gctgagctga gaatatcaag actgtaccga gtagggggcc ttggcaagtg tggagagccc
      301 ggcagctggg gcagagggcg gagtacggtg tgcgtttacg gacctcttca aacgaggtag
      361 gaaggtcaga agtcaaaaag ggaacaaatg atgtttaacc acacaaaaat gaaaatccaa
      421 tggttggata tccattccaa atacacaaag gcaacggata agtgatccgg gccaggcaca
      481 gaaggccatg cacccgtagg attgcactca gagctcccaa atgcatagga atagaagggt
      541 gggtgcagga ggctgagggg tggggaaagg gcatgggtgt ttcatgagga cagagcttcc
      601 gtttcatgca atgaaaagag tttggagacg gatggtggtg actggactat acacttacac
      661 acggtagcga tggtacactt tgtattatgt atattttacc acgatctttt taaagtgtca
      721 aaggcaaatg gccaaatggt tccttgtcct atagctgtag cagccatcgg ctgttagtga
      781 caaagcccct gagtcaagat gacagcagcc cccataactc ctaatcggct ctcccgcgtg
      841 gagtcattta ggagtagtcg cattagagac aagtccaaca tctaatcttc caccctggcc
      901 agggccccag ctggcagcga gggtgggaga ctccgggcag agcagagggc gctgacattg
      961 gggcccggcc tggcttgggt ccctctggcc tttccccagg ggccctcttt ccttggggct
     1021 ttcttgggcc gccactgctc ccgctcctct ccccccatcc caccccctca ccccctcgtt
     1081 cttcatatcc ttctctagtg ctccctccac tttcatccac ccttctgcaa gagtgtggga
     1141 ccacaaatga gttttcacct ggcctgggga cacacgtgcc cccacaggtg ctgagtgact
     1201 ttctaggaca gtaatctgct ttaggctaaa atgggacttg atcttctgtt agccctaatc
     1261 atcaattagc agagccggtg aaggtgcaga acctaccgcc tttccaggcc tcctcccacc
     1321 tctgccacct ccactctcct tcctgggatg tgggggctgg cacacgtgtg gcccagggca
     1381 ttggtgggat tgcactgagc tgggtcatta gcgtaatcct ggacaagggc agacagggcg
     1441 agcggagggc cagctccggg gctcaggcaa ggctgggggc ttcccccaga caccccactc
     1501 ctcctctgct ggacccccac ttcatagggc acttcgtgtt ctcaaagggc ttccaaatag
     1561 catggtggcc ttggatgccc agggaagcct cagagttgct tatctccctc tagacagaag
     1621 gggaatctcg gtcaagaggg agaggtcgcc ctgttcaagg ccacccagcc agctcatggc
     1681 ggtaatggga caaggctggc cagccatccc accctcagaa gggacccggt ggggcaggtg
     1741 atctcagagg aggctcactt ctgggtctca cattcttgga tccggttcca ggcctcggcc
     1801 ctaaatagtc tccctgggct ttcaagagaa ccacatgaga aaggaggatt cgggctctga
     1861 gcagtttcac cacccacccc ccagtctgca aatcctgacc cgtgggtcca cctgccccaa
     1921 aggcggacgc aggacagtag aagggaacag agaacacata aacacagaga gggccacagc
     1981 ggctcccaca gtcaccgcca ccttcctggc ggggatgggt ggggcgtctg agtttggttc
     2041 ccagcaaatc cctctgagcc gcccttgcgg gctcgcctca ggagcagggg agcaagaggt
     2101 gggaggagga ggtctaagtc ccaggcccaa ttaagagatc aggtagtgta gggtttggga
     2161 gcttttaagg tgaagaggcc cgggctgatc ccacaggcca gtataaagcg ccgtgaccct
     2221 caggtgatgc gccagggccg gctgccgtcg gggacagggc tttccatagc ggccgcgccg
     2281 ccaccatggg cagtctggta ttcaggagac ctttctgcca tctctccacc tacagcctga
     2341 tttggggcat ggcagcagta gcgctgagca cagctcaagt ggaagtggtg acccaggatg
     2401 aaagaaaggc gctgcacaca actgcatcct tacgatgttc tctaaaaaca tcccaggaac
     2461 ccttgattgt gacatggcag aaaaagaaag ccgtgagccc agaaaacatg gtcacctaca
     2521 gcaaaaccca tggggttgta atccagcctg cctacaaaga caggataaat gtcacagagc
     2581 tgggactctg gaactcaagc atcaccttct ggaacacaac attggaagat gagggctgct
     2641 acatgtgtct cttcaacacg tttggttctc agaaggtctc aggaacagct tgccttaccc
     2701 tctatgtaca gcccatagta caccttcact acaactattt tgaagaccac ctaaacatca
     2761 cttgctctgc gactgcccgt ccagcccctg ccatctcctg gaagggtact gggacaggaa
     2821 ttgagaatag taccgagagt cacttccatt caaatgggac tacatctgtc accagcatcc
     2881 tccgggtcaa agaccccaaa actcaagttg ggaaggaagt gatctgccag gttttatacc
     2941 tggggaatgt gattgactac aagcagagtc tggacaaagg attttggttt tcagttccac
     3001 tgttgctaag cattgtttct ctggtaattc ttctgatctt gatctccatc ttactatact
     3061 ggaaacgtca ccgaaatcag gagcggggtg aatcatcaca ggggatgcaa agaatgaaat
     3121 aaaccggtaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3181 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3241 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3301 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3361 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3421 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3481 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     3541 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3601 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3661 cgcgtcttcg agatctgcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     3721 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     3781 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     3841 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg actcgagtta
     3901 agggcgaatt cccgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
     3961 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4021 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4081 cggcctcagt gagcgagcga gcgcgcagcc ttaattaacc taattcactg gccgtcgttt
     4141 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4201 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4261 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4321 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4381 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4441 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4501 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4561 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4621 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4681 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4861 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4921 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4981 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5041 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5101 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5161 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5221 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5281 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5341 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5401 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5461 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5521 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5581 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5641 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5701 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5761 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5821 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5881 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5941 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6001 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6061 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6121 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6181 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6241 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6301 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6361 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6421 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6481 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6541 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6601 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6661 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6721 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6781 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6841 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6901 cgccagattt aattaaggcc ttaatt
//
