LOCUS       sequence-324071-       10229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hsp70 promoter inducible expression of 
            DHB:mScarlet-P2A-H2B:miRFP670.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10229)
  AUTHORS   Adikes RC, Kohrman AQ, Martinez MAQ, Palmisano NJ, Smith JJ, 
            Medwig-Kinney TN, Min M, Sallee MD, Ahmed OB, Kim N, Liu S, Morabito
            RD, Weeks N, Zhao Q, Zhang W, Feldman JL, Barkoulas M, Pani AM, 
            Spencer SL, Martin BL, Matus DQ
  TITLE     Visualizing the metazoan proliferation-quiescence decision in vivo.
  JOURNAL   Elife. 2020 Dec 22;9. pii: 63265. doi: 10.7554/eLife.63265.
  PUBMED    33350383
REFERENCE   2  (bases 1 to 10229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-12-22"; volume: "9. pii: 63265. doi"; pages: " 
            10.7554/eLife.63265"
COMMENT     Sequence Label: pRM15
FEATURES             Location/Qualifiers
     source          1..10229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2494..2505,2506..2668)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     3037..3053
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4623..4641
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4623..4640
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             4979..5674
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(5112..5130)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5380..5399
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5684..5740
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5741..6118
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      6131..6140
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6137..7084
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     CDS             7099..7155
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    7193..7327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7217..7236)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7271..7290
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7751..7938,7939..7950)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7984..8000)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8041..8061)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8041..8059)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8080..8096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8080..8096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8093..8115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8104..8120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8128..8134,8135..8152,8153..8158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8173..8194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8311..8328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8482..9070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8562..8581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9241..10032,10033..10101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9864..9883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10102..10206)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccaa
      661 atagtaggaa ttacccacct gtacaagtgc tgaaaacttg gatgaataag cccgtttgcc
      721 attttctact gctattttaa atcttttctg tatttgtctg catttgtctt tacccttgaa
      781 taaatgtttt agttgttttt tttcaattat gactgtgttt aacagacatt attacaataa
      841 tatgtaatag tagtacacac tattattatg taatagtact tgttgactgt atttgagaac
      901 tggaccgagt gagtgttacg tcacccattc aaaatgactt acttctggct ccaacaaaat
      961 gaagttaatt cagttgccat ttttcactgt atggacatcg ccgtgttgga gctagacgtt
     1021 gccaataagc aagaaagagc cgagatgcgt cgagtctgag tcaccgttcc tatggcaacc
     1081 cctctaacca atcagaagta agcttgttgg aagtccacag cctaccactt gaaagcgggc
     1141 tgcacaaaat ctgtcaaacg ttttgaacgt tggatgtgag agcacatact tttattaagg
     1201 catctggttg gtcagtttat aatatcaaca acttgggcta cagaaaagaa aagttattac
     1261 agaaattatg attaacaagt acatgttaaa taaagatttt aatatgaatg ccaccactgg
     1321 agcattcatg ccatttggag cttcttcctg tttggatcac tagaaggagg aggtcactca
     1381 ttacagttct catatacagt cgttggttgg ttggttggtt ggtagattga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 ggtagtcaaa ataagaaata atttccacag attcattaca gaaatgatta aatgcataca
     1561 taaaaaactg gggggggggg gatacaacaa cacacttaag tcacatttgc ctacgtaaag
     1621 aaaagtaaag aaaatcaata gctatatttt acatctcctt tttttgctgt cttttaatta
     1681 gccttgtttt gctgttatct tgattgaaac tgtaacttct tcacctgctt cttttctttg
     1741 taggttttgc cagcccagaa acaggcatgg ctgtgcaagg ccagagcacc atgcacaatg
     1801 cgctgcatgt cttcatgaac ggctcaatgt cctcagtcca gggctcagcc aacgacccca
     1861 tcttcctcct tcaccatgct ttcattgaca ggtaacaaac acgtcatgac attagactgc
     1921 acagtttttg acaaagttca tacaatctgt tgtttatagc tgctacaatt agtgaagttt
     1981 gtgaatgtac ttggatgagc agcgaaagat caattgagat caattgttag agtttggttg
     2041 ccctgcagag caaagaacaa aaaataatct ggtggcttta ctgcgtgagg ttattattgg
     2101 tggaatagaa acacaaaaca taattgcatt tatttgttta attttttatc ttatcttaac
     2161 tttcatcttg catatttgtt tcttacatca tttctagcat ctttgagcgc tggctaagaa
     2221 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
     2281 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
     2341 atgctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
     2401 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
     2461 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
     2521 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
     2581 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
     2641 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
     2701 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
     2761 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
     2821 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
     2881 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
     2941 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     3001 ctccctggtg tctgaaacac aggccagatg ggccgctcta gaactagtgg atcttaggga
     3061 taacagggta atgtcgagat ccttcagggg tgtcgcttgg ttatttccaa aaatcaaatt
     3121 aattttatta aactattaga acgagcatgt tttgtctata tgctacagaa gataaaaaat
     3181 aataggagtt aacagttata aaacaacaca ctttgtttct attgattgtt gaccacactg
     3241 gggtctcatt aagttagatt aaagacacac taactgggtc aaaagcagca gattgatttc
     3301 atagcaccag ggtaaacttt ctaacacttt tacggcaatc atatacatta aaattaaata
     3361 cagaccacga ctgaacaagg aggatgatct ccaatattaa acaaagagac ttgtgcctat
     3421 ttctctgagg gtaaacatga cctctcaagt tagcaagttg tttttaacac tacaaaaata
     3481 gttaagactg caatcccaga ataaagtatt ggttttaacc aatcaatata gtacagtaaa
     3541 catccatttg ttttgttgaa acgttaaaca aatctgacca aagctattag cttatataaa
     3601 acaggtttgc cttctatgta gctgaaaaca ccacaggccc gattttgcta ctgtgtaaaa
     3661 catttcagca agattttttt attgcatttt tttttactga atcgttcaaa cattttatca
     3721 ttttagtttg ttcattcatt gcaactggaa aaacaacaca tcacacaacc gcacatattt
     3781 cagcaataag tacaataaaa cactcaaata aaaaaaacat tttaaatctc tttgtatttt
     3841 tgaccgctgt ttcgcgtaat ttcacggtaa aactctggaa atctccacta cattcctctc
     3901 agcggctcct ctcaatgaca gctgaagaag tgacgcggct gcctgctgtg ttttgattgg
     3961 tcgaattcac tggaggcttc cagaacagtg tagagtctga acgggtgcgc gctctgctgt
     4021 atttaaaggg cgaaagagag accgcagaga aactcaaccg aagagaagcg acttgacaaa
     4081 gaagaaaaga gcagcctgac aggacttttt ccccgacgag gtgtttattc gctctattta
     4141 agaatctact gtaaggtaag tctcaatata ttgtactcta ttggctaatc agaattatat
     4201 agagattata tgtacttaat gtcaaaaaat caactttgta tatgtaatct ttttacatgt
     4261 ggactgccta tgttcatctt attttaggtc tactagaaaa ttatatttcc cgttttcaca
     4321 ataaggattt ttaaaaaaag caatgaacag acgggcattt actttatgtt gctgacatta
     4381 ttttatatga gcataataac cataaatact agcaaatgtc ctaaatgaat ttgtgttaat
     4441 gttgtctaca aaagaaaatt agcgttttac ttgtacaact aataataact tggttattaa
     4501 gagaatttca cttgttgact agaaaaatcc tttcataatg aaacaattgc accataaatt
     4561 gtataaatat aaaattaatt ctaattgttt ttttttcctg caggaattcg atatcaagct
     4621 tgatttaggt gacactatag aatacaagct acttgttctt tttgcaggat ccatgacaaa
     4681 tgatgtcacc tggagcgagg cctcttcgcc tgatgagagg acactcacct ttgctgaaag
     4741 atggcaatta tcttcacctg atggagtaga tacagatgat gatttaccaa aatcgcgagc
     4801 atccaaaaga acctgtggtg tgaatgatga tgaaagtcca agcaaaattt ttatggtggg
     4861 agaatctcca caagtgtctt ccagacttca gaatttgaga ctgaataatt taattcccag
     4921 gcaacttttc aagcccaccg ataatcaaga aactggttcc ggggcccagg gcagcggcat
     4981 ggtgagcaag ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg
     5041 ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5101 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat
     5161 cctgtcccct cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc
     5221 cgactactat aagcagtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5281 ggacggcggc gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta
     5341 caaggtgaag ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac
     5401 aatgggctgg gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga
     5461 cattaagatg gccctgcgcc tgaaggacgg cggccgctac ctggcggact tcaagaccac
     5521 ctacaaggcc aagaagcccg tgcagatgcc cggcgcctac aacgtcgacc gcaagttgga
     5581 catcacctcc cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg
     5641 acactccacc ggcggcatgg acgagctgta caagggcagc ggcgccacca acttcagcct
     5701 gctgaagcag gccggcgacg tggaggagaa ccccggcccc atgccagagc cagcgaagtc
     5761 tgctcccgcc ccgaaaaagg gctccaagaa ggcggtgact aaggcgcaga agaaaggcgg
     5821 caagaagcgc aagcgcagcc gcaaggagag ctattccatc tatgtgtaca aggttctgaa
     5881 gcaggtccac cctgacaccg gcatttcgtc caaggccatg ggcatcatga attcgtttgt
     5941 gaacgacatt ttcgagcgca tcgcaggtga ggcttcccgc ctggcgcatt acaacaagcg
     6001 ctcgaccatc acctccaggg agatccagac ggccgtgcgc ctgctgctgc ctggggagtt
     6061 ggccaagcac gccgtgtccg agggtactaa ggccatcacc aagtacacca gcgctaaggt
     6121 tccaccggtc gccaccatgg tagcaggtca tgcctctggc agccccgcat tcgggaccgc
     6181 ctctcattcg aattgcgaac atgaagagat ccacctcgcc ggctcgatcc agccgcatgg
     6241 cgcgcttctg gtcgtcagcg aacatgatca tcgcgtcatc caggccagcg ccaacgccgc
     6301 ggaatttctg aatctcggaa gcgtactcgg cgttccgctc gccgagatcg acggcgatct
     6361 gttgatcaag atcctgccgc atctcgatcc caccgccgaa ggcatgccgg tcgcggtgcg
     6421 ctgccggatc ggcaatccct ctacggagta ctgcggtctg atgcatcggc ctccggaagg
     6481 cgggctgatc atcgaactcg aacgtgccgg cccgtcgatc gatctgtcag gcacgctggc
     6541 gccggcgctg gagcggatcc gcacggcggg ttcactgcgc gcgctgtgcg atgacaccgt
     6601 gctgctgttt cagcagtgca ccggctacga ccgggtgatg gtgtatcgtt tcgatgagca
     6661 aggccacggc ctggtattct ccgagtgcca tgtgcctggg ctcgaatcct atttcggcaa
     6721 ccgctatccg tcgtcgactg tcccgcagat ggcgcggcag ctgtacgtgc ggcagcgcgt
     6781 ccgcgtgctg gtcgacgtca cctatcagcc ggtgccgctg gagccgcggc tgtcgccgct
     6841 gaccgggcgc gatctcgaca tgtcgggctg cttcctgcgc tcgatgtcgc cgtgccatct
     6901 gcagttcctg aaggacatgg gcgtgcgcgc caccctggcg gtgtcgctgg tggtcggcgg
     6961 caagctgtgg ggcctggttg tctgtcacca ttatctgccg cgcttcatcc gtttcgagct
     7021 gcgggcgatc tgcaaacggc tcgccgaaag gatcgcgacg cggatcaccg cgcttgagag
     7081 ctaaatcgat tctctggagc cacgaacttc tctctgttaa agcaagcagg agacgtggaa
     7141 gaaaaccccg gtcctgtcga gcctctagaa ctatagtgag tcgtattacg tagatccaga
     7201 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     7261 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     7321 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     7381 ggttttttaa ttcgcggccg cgtcgagggg gggcccgcta gcatatgaga tctgcgaaga
     7441 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     7501 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     7561 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     7621 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     7681 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     7741 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     7801 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     7861 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     7921 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     7981 gcagatccac tagttctaga gcggccggcc gccaccgcgg tggagctcca gcttttgttc
     8041 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     8101 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     8161 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     8221 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8281 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8341 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8401 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8461 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8521 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8581 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8641 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8701 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8761 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8821 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8881 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8941 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     9001 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     9061 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     9121 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     9181 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9241 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9301 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9361 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9421 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9481 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9541 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9601 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9661 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9721 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9781 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9841 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9901 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9961 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    10021 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    10081 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    10141 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    10201 tccgcgcaca tttccccgaa aagtgccac
//
