LOCUS       sequence-324075-        7326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid expressing TGFB2 in photoreceptors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Wang SK, Xue Y, Cepko CL
  TITLE     Microglia modulation by TGF-beta1 protects cones in mouse models of 
            retinal degeneration.
  JOURNAL   J Clin Invest. 2020 Aug 3;130(8):4360-4369. doi: 10.1172/JCI136160.
  PUBMED    32352930
REFERENCE   2  (bases 1 to 7326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Clin Invest"; date: "2020-08-3- 3"; volume: "130"; issue: "8"; 
            pages: "4360-4369"
COMMENT     Sequence Label: AAV-hRedOpsin-TGFB2
FEATURES             Location/Qualifiers
     source          1..7326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    104..125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(140..145,146..163,164..170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     183..205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     202..218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     202..218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   256..385
                     /label=AAV2 ITR
     primer_bind     complement(3834..3854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4252..4263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4330..4347)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4336..4543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4631..4760
                     /label=AAV2 ITR
     primer_bind     complement(4779..4796)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4779..4795)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4788..4810)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4937..5392
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5024..5043)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5234..5255
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5418..5522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5523..5591,5592..6383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5741..5760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6554..7142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7043..7062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
       61 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      121 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt
      241 aaggccttaa ttaggctgcg cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg
      301 cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag agagggagtg
      361 gccaactcca tcactagggg ttccttgtag ttaatgatta acccgccatg ctacttatct
      421 acgtagccat gctctaggaa gatcggaatt cgcccttaag ctagcctaca gcagccaggg
      481 tgagattatg aggctgagct gagaatatca agactgtacc gagtaggggg ccttggcaag
      541 tgtggagagc ccggcagctg gggcagaggg cggagtacgg tgtgcgttta cggacctctt
      601 caaacgaggt aggaaggtca gaagtcaaaa agggaacaaa tgatgtttaa ccacacaaaa
      661 atgaaaatcc aatggttgga tatccattcc aaatacacaa aggcaacgga taagtgatcc
      721 gggccaggca cagaaggcca tgcacccgta ggattgcact cagagctccc aaatgcatag
      781 gaatagaagg gtgggtgcag gaggctgagg ggtggggaaa gggcatgggt gtttcatgag
      841 gacagagctt ccgtttcatg caatgaaaag agtttggaga cggatggtgg tgactggact
      901 atacacttac acacggtagc gatggtacac tttgtattat gtatatttta ccacgatctt
      961 tttaaagtgt caaaggcaaa tggccaaatg gttccttgtc ctatagctgt agcagccatc
     1021 ggctgttagt gacaaagccc ctgagtcaag atgacagcag cccccataac tcctaatcgg
     1081 ctctcccgcg tggagtcatt taggagtagt cgcattagag acaagtccaa catctaatct
     1141 tccaccctgg ccagggcccc agctggcagc gagggtggga gactccgggc agagcagagg
     1201 gcgctgacat tggggcccgg cctggcttgg gtccctctgg cctttcccca ggggccctct
     1261 ttccttgggg ctttcttggg ccgccactgc tcccgctcct ctccccccat cccaccccct
     1321 caccccctcg ttcttcatat ccttctctag tgctccctcc actttcatcc acccttctgc
     1381 aagagtgtgg gaccacaaat gagttttcac ctggcctggg gacacacgtg cccccacagg
     1441 tgctgagtga ctttctagga cagtaatctg ctttaggcta aaatgggact tgatcttctg
     1501 ttagccctaa tcatcaatta gcagagccgg tgaaggtgca gaacctaccg cctttccagg
     1561 cctcctccca cctctgccac ctccactctc cttcctggga tgtgggggct ggcacacgtg
     1621 tggcccaggg cattggtggg attgcactga gctgggtcat tagcgtaatc ctggacaagg
     1681 gcagacaggg cgagcggagg gccagctccg gggctcaggc aaggctgggg gcttccccca
     1741 gacaccccac tcctcctctg ctggaccccc acttcatagg gcacttcgtg ttctcaaagg
     1801 gcttccaaat agcatggtgg ccttggatgc ccagggaagc ctcagagttg cttatctccc
     1861 tctagacaga aggggaatct cggtcaagag ggagaggtcg ccctgttcaa ggccacccag
     1921 ccagctcatg gcggtaatgg gacaaggctg gccagccatc ccaccctcag aagggacccg
     1981 gtggggcagg tgatctcaga ggaggctcac ttctgggtct cacattcttg gatccggttc
     2041 caggcctcgg ccctaaatag tctccctggg ctttcaagag aaccacatga gaaaggagga
     2101 ttcgggctct gagcagtttc accacccacc ccccagtctg caaatcctga cccgtgggtc
     2161 cacctgcccc aaaggcggac gcaggacagt agaagggaac agagaacaca taaacacaga
     2221 gagggccaca gcggctccca cagtcaccgc caccttcctg gcggggatgg gtggggcgtc
     2281 tgagtttggt tcccagcaaa tccctctgag ccgcccttgc gggctcgcct caggagcagg
     2341 ggagcaagag gtgggaggag gaggtctaag tcccaggccc aattaagaga tcaggtagtg
     2401 tagggtttgg gagcttttaa ggtgaagagg cccgggctga tcccacaggc cagtataaag
     2461 cgccgtgacc ctcaggtgat gcgccagggc cggctgccgt cggggacagg gctttccata
     2521 gcggccgcca tgcactactg tgtgctgagc acctttttgc tcctgcatct ggtcccggtg
     2581 gcgctcagtc tgtctacctg cagcaccctc gacatggatc agtttatgcg caagaggatc
     2641 gaggccatcc gcgggcagat cctgagcaag ctgaagctca ccagcccccc ggaagactat
     2701 ccggagccgg atgaggtccc cccggaggtg atttccatct acaacagtac cagggactta
     2761 ctgcaggaga aggcaagccg gagggcagcc gcctgcgagc gcgagcggag cgacgaggag
     2821 tactacgcca aggaggttta taaaatcgac atgccgtccc acctcccctc cgaaaatgcc
     2881 atcccgccca ctttctacag accctacttc agaatcgtcc gctttgatgt ctcaacaatg
     2941 gagaaaaatg cttcgaatct ggtgaaggca gagttcaggg tcttccgctt gcaaaacccc
     3001 aaagccagag tggccgagca gcggattgaa ctgtatcaga tccttaaatc caaagactta
     3061 acatctccca cccagcgcta catcgatagc aaggttgtga aaaccagagc ggagggtgaa
     3121 tggctctcct tcgacgtgac agacgctgtg caggagtggc ttcaccacaa agacaggaac
     3181 ctggggttta aaataagttt acactgcccc tgctgtacct tcgtgccgtc taataattac
     3241 atcatcccga ataaaagcga agagctcgag gcgagatttg caggtattga tggcacctct
     3301 acatatgcca gtggtgatca gaaaactata aagtccacta ggaaaaaaac cagtgggaag
     3361 accccacatc tcctgctaat gttgttgccc tcctacagac tggagtcaca acagtccagc
     3421 cggcggaaga agcgcgcttt ggatgctgcc tactgcttta gaaatgtgca ggataattgc
     3481 tgccttcgcc ctctttacat tgattttaag agggatcttg gatggaaatg gatccatgaa
     3541 cccaaagggt acaatgctaa cttctgtgct ggggcatgcc catatctatg gagttcagac
     3601 actcaacaca ccaaagtcct cagcctgtac aacaccataa atcccgaagc ttccgcttcc
     3661 ccttgctgtg tgtcccagga tctggaacca ctgaccattc tctattacat tggaaatacg
     3721 cccaagatcg aacagctttc caatatgatt gtcaagtctt gtaaatgcag ctaaaccggt
     3781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3841 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3901 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3961 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4021 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4081 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4141 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     4201 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4261 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4321 cgagatctgc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggactcgagt taagggcgaa
     4561 ttcccgataa ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc
     4621 attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4681 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4741 gtgagcgagc gagcgcgcag ccttaattaa cctaattcac tggccgtcgt tttacaacgt
     4801 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     4861 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     4921 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4981 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     5041 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     5101 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     5161 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     5221 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     5281 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     5341 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     5401 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5461 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5521 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5581 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5641 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5701 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5761 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5821 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5881 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5941 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6001 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6061 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6121 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6181 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6241 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6301 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6361 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6421 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6481 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6541 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6601 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6661 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6721 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6781 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6841 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6901 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6961 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7021 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7081 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7141 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7201 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7261 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7321 aagagc
//
