LOCUS       sequence-324081-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescently tagged affinity reagent.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, 
            Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig
            E, Chew TL, Adalsteinsson D, Hahn KM
  TITLE     Software for lattice light-sheet imaging of FRET biosensors, 
            illustrated with a new Rap1 biosensor.
  JOURNAL   J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 
            10.1083/jcb.201903019. Epub 2019 Aug 23.
  PUBMED    31444239
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201903019"; journalName: "J Cell Biol"; date: 
            "2019-09-2- 2"; volume: "218"; issue: "9"; pages: "3153-3160"
COMMENT     Sequence Label: RalGDS-YPet
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(604..623)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctagcatgg actgctgtat catccgcgtc agcctggacg tggacaatgg caacatgtac
       61 aagagcatcc tggtgaccag ccaagataag gctccggctg taatccgcaa ggccatggac
      121 aaacacaacc tggaggagga ggagccggag gactatgagc tgctgcagat tctctcagat
      181 gaccggaagc tgaagatccc tgaaaacgcc aacgtcttct atgccatgaa ctctaccgcc
      241 aactatgact ttgtcctcaa gaagcggacc ttctccggag gatccggttc actcgagatg
      301 gtgagcaagg gcgaagagct gttcaccggc gtggtgccca tcctggtgga gctggacggc
      361 gacgtgaacg gccacaagtt cagcgtgagc ggcgagggcg agggcgacgc cacctacggc
      421 aagctgaccc tgaagctgct gtgcaccacc ggcaagctgc ccgtgccctg gcccaccctg
      481 gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccggt accccgacca catgaagcag
      541 cacgacttct tcaagagcgc catgcccgag ggctacgtgc aggagcggac catcttcttc
      601 aaggacgacg gcaactacaa gacccgggcc gaggtgaagt tcgagggcga caccctggtg
      661 aaccggatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct gggccacaag
      721 ctggagtaca actacaacag ccacaacgtg tacatcaccg ccgacaagca gaagaacggc
      781 atcaaggcca acttcaagat ccggcacaac atcgaggacg gcggcgtgca gctggccgac
      841 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
      901 ctgagctacc agagcgccct gttcaaggac cccaacgaga agcgggacca catggtgctg
      961 ctggagttcc tgaccgccgc cggcatcacc gagggcatgg acgagctgta caaggaattc
//
