LOCUS       sequence-324086-        1311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescently tagged GTPase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, 
            Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig
            E, Chew TL, Adalsteinsson D, Hahn KM
  TITLE     Software for lattice light-sheet imaging of FRET biosensors, 
            illustrated with a new Rap1 biosensor.
  JOURNAL   J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 
            10.1083/jcb.201903019. Epub 2019 Aug 23.
  PUBMED    31444239
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201903019"; journalName: "J Cell Biol"; date: 
            "2019-09-2- 2"; volume: "218"; issue: "9"; pages: "3153-3160"
COMMENT     Sequence Label: mCerulean3-Rap1
FEATURES             Location/Qualifiers
     source          1..1311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(7..9,10..12,13..723)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(52..73)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(313..332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     660..681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctagcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
       61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      181 cccaccctcg tgaccaccct gtcctggggc gtgcagtgct tcgcccgcta ccccgaccac
      241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      421 gggcacaagc tggagtacaa cgccatccac ggcaacgtct atatcaccgc cgacaagcag
      481 aagaacggca tcaaggccaa cttcggcctc aactgcaaca tcgaggacgg cagcgtgcag
      541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      601 aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac
      661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      721 aagtccggac tcagatctcg agctaagctt atgcgtgagt ataagctagt cgttcttggc
      781 tcaggaggcg ttggaaagtc tgctttgact gtacaatttg ttcaaggaat ttttgtagaa
      841 aaatacgatc ctacgataga agattcttat agaaagcaag ttgaagtaga tgcacaacag
      901 tgtatgcttg aaatcttgga tactgcagga acggagcaat ttacagcaat gagggattta
      961 tacatgaaaa atggacaagg atttgcatta gtttattcca tcacagcaca gtccacattt
     1021 aacgatttac aagacctgag agaacagatt cttcgagtta aagacactga tgatgttcca
     1081 atgattcttg ttggtaataa gtgtgacttg gaagatgaaa gagttgtagg gaaggaacaa
     1141 ggtcaaaatc tagcaagaca atggaacaac tgtgcattct tagaatcttc tgcaaaatca
     1201 aaaataaatg ttaatgagat cttttatgac ctagtgcggc aaattaacag aaaaactcca
     1261 gtgcctggga aggctcgcaa aaagtcatca tgtcagctgc tttaagaatt c
//
