LOCUS       sequence-324122-        6557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for multigene expression to generate recombinant 
            baculoviruses by homologous recombination. Contains a p10/pH dual 
            expression cassette with an C-ter10His tag under the p10 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Kolesnikova O, Zachayus A, Pichard S, Osz J, Rochel N, Rossolillo P,
            Kolb-Cheynel I, Troffer-Charlier N, Compe E, Bensaude O, Berger I, 
            Poterszman A
  TITLE     HR-Bac, a toolbox based on homologous recombination for expression, 
            screening and production of multiprotein complexes using the 
            baculovirus expression system.
  JOURNAL   Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.1038/s41598-021-04715-5.
  PUBMED    35132103
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-02-7"; volume: "12(1):2030. doi"; pages: " 
            10.1038/s41598-021-04715-5"
COMMENT     Sequence Label: pAC8_MF-PH-10His-Cter (VE5631)
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    46..180
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(83..102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     137..156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_recomb     299..1656
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(315..1656)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      1803..2258
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1890..1909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2100..2121
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2275..2294)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2394..2416
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2454..2472)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2540..2644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2645..2713,2714..3505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2863..2882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3676..4264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4165..4184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4418..4435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     4827..5654
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             4827..4951
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(4989..5594)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(5661..5694)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5884..5932
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5957..5976
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(6068..6177)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        6222..6313
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     6238..6255
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             6428..6457
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
       61 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      181 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
      241 ggctgctgcc accgctgagc aataactatc ataaccccta ggcgattaat tgatccgggt
      301 tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga caattgttgt
      361 acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag agcgaaaatc
      421 aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat agatttgtaa
      481 aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc tggactatct
      541 aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa tctagctttg
      601 tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa aatattatgc
      661 gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt aaacacgtta
      721 aataaagctt ggacatattt aacatcgggc gtgttagctt tattaggccg attatcgtcg
      781 tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat agccacacga
      841 cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga gctttttgga
      901 attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc cgattttaat
      961 tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga cggcaaatct
     1021 actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg cgcaggcggg
     1081 gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg tttaggctca
     1141 aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc gggcgccgtt
     1201 tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc ttccaacaat
     1261 tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg tggagcgggc
     1321 ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt aggcacggga
     1381 gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg ttcgcgcacg
     1441 attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc
     1501 agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta aaaatctgct
     1561 ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa
     1621 gcaattgtat tgtaaagaga ttgtctcaag ctcggatcga tcccgcacgc cgataacaag
     1681 ccttttcatt tttactacag cattgtagtg gcgagacact tcgctgtcgt cgcctgatgc
     1741 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     1801 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     1861 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     1921 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     1981 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     2041 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     2101 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     2161 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     2221 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     2281 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     2341 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     2401 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     2461 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2521 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2581 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2641 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2701 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2761 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2821 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2881 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2941 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3001 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3061 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3121 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3181 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3241 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3301 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3361 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3421 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3481 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3541 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3601 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3661 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3721 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3781 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3841 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3901 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3961 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4021 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4081 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4141 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4201 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4261 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4321 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4381 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4441 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4501 aacggctccg cccactatta atgaaattaa aaattccaat tttaaaaaac gcagcaagag
     4561 aaacatttgt atgaaagaat gcgtagaagg aaagaaaaat gtcgtcgaca tgctgaacaa
     4621 caagattaat atgcctccgt gtataaaaaa aatattgaac gatttgaaag aaaacaatgt
     4681 accgcgcggc ggtatgtaca ggaagaggtt tatactaaac tgttacattg caaacgtggt
     4741 ttcgtgtgcc aagtgtgaaa accgatgttt aatcaaggct ctgacgcatt tctacaacca
     4801 cgactccaag tgtgtgggtg aagtcatgca tcttttaatc aaatcccaag atgtgtataa
     4861 accaccaaac tgccaaaaaa tgaaaactgt cgacaagctc tgtccgtttg ctggcaactg
     4921 caagggtctc aatcctattt gtaattattg aataataaaa caattataaa tgtcaaattt
     4981 gttttttatt aacgatacaa accaaacgca acaagaacat ttgtagtatt atctataatt
     5041 gaaaacgcgt agttataatc gctgaggtaa tatttaaaat cattttcaaa tgattcacag
     5101 ttaatttgcg acaatataat tttattttca cataaactag acgccttgtc gtcttcttct
     5161 tcgtattcct tctctttttc atttttctcc tcataaaaat taacatagtt attatcgtat
     5221 ccatatatgt atctatcgta tagagtaaat tttttgttgt cataaatata tatgtctttt
     5281 ttaatggggt gtatagtacc gctgcgcata gtttttctgt aatttacaac agtgctattt
     5341 tctggtagtt cttcggagtg tgttgcttta attattaaat ttatataatc aatgaatttg
     5401 ggatcgtcgg ttttgtacaa tatgttgccg gcatagtacg cagcttcttc tagttcaatt
     5461 acaccatttt ttagcagcac cggattaaca taactttcca aaatgttgta cgaaccgtta
     5521 aacaaaaaca gttcacctcc cttttctata ctattgtctg cgagcagttg tttgttgtta
     5581 aaaataacag ccattgtaat gagacgcaca aactaatatc acaaactgga aatgtctatc
     5641 aatatatagt tgctgatatc ataacttcgt atagcataca ttatacgaag ttatctggtt
     5701 taaacgtacc cgtagtggct atggcagggc ttgccgcccc gacgttggct gcgagccctg
     5761 ggccttcacc cgaacttggg ggttggggtg gggaaaagga agaaacgcgg gcgtattggt
     5821 cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga accccgcgtt
     5881 tatgaacaaa cgacccaaca cccgtgcgtt ttattctgtc tttttattgc cgtcatagcg
     5941 cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccca tctcccggta
     6001 ccgcatgcta tgcatcagct gctagcacca tggctcgaga tcccgggtga tcaagtcttc
     6061 gtcgagtgat tgtaaataaa atgtaattta cagtatagta ttttaattaa tatacaaatg
     6121 atttgataat aattcttatt taactataat atattgtgtt gggttgaatt aaaggtccgt
     6181 atactagtat cgattcgcga cctactccgg aatattaata gatcatggag ataattaaaa
     6241 tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt ttgtaataaa
     6301 aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg gatcccggtc
     6361 cgaagcgcgc ggaattcaaa ggcctacgtc gacgagctca ctagtcgcgg ccgctttcga
     6421 atctagacac catcatcacc atcaccatca ccaccattaa ctgcagtctc gacaagcttg
     6481 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     6541 taaaaaacct cccacac
//
