LOCUS       sequence-324128-        2241 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the fusion protein of human MSI2 and the hyperactive 
            catalytic domain of Drosophila ADAR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Nguyen DTT, Lu Y, Chu KL, Yang X, Park SM, Choo ZN, Chin CR, Prieto 
            C, Schurer A, Barin E, Savino AM, Gourkanti S, Patel P, Vu LP, 
            Leslie CS, Kharas MG
  TITLE     HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and 
            differential regulation in leukemic stem cells.
  JOURNAL   Nat Commun. 2020 Apr 24;11(1):2026. doi: 10.1038/s41467-020-15814-8.
  PUBMED    32332729
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-24"; volume: "11(1):2026. doi"; pages: " 
            10.1038/s41467-020-15814-8"
COMMENT     Sequence Label: MSI2-ADA
FEATURES             Location/Qualifiers
     source          1..2241
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2191..2238
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 atggaagcca acggcagcca gggcacctcc ggctccgcca atgacagcca acacgacccc
       61 ggaaagatgt tcatcggcgg cctcagctgg cagaccagcc ccgatagcct gagggactac
      121 ttctccaaat tcggcgaaat tagggagtgc atggtgatga gagaccccac aaccaagagg
      181 tccaggggct tcggctttgt caccttcgcc gatcccgctt ccgtggacaa ggtcctgggc
      241 cagccccatc atgagctgga ctccaagaca atcgacccca aagtggcctt tcccaggaga
      301 gcccagccca agatggtgac caggaccaag aagatcttcg tgggcggcct gagcgctaac
      361 accgtggtgg aggacgtcaa gcaatacttc gagcagttcg gaaaggtcga agacgccatg
      421 ctgatgttcg acaagacaac caataggcac agaggcttcg gcttcgtcac cttcgagaac
      481 gaggatgtcg tcgagaaggt gtgcgagatt cacttccacg aaattaacaa taagatggtc
      541 gagtgcaaga aggctcagcc taaagaggtc atgttccccc ccggcacaag aggcagagct
      601 aggggcctcc cctatacaat ggacgccttc atgctgggca tgggcatgct gggctacccc
      661 aactttgtgg ctacatacgg caggggctat cccggcttcg ccccctccta tggctaccag
      721 ttccccggat tccccgctgc tgcttacgga cccgtggccg ccgccgccgt ggctgccgcc
      781 agaggcagcg gatccaatcc cgctaggcct ggcggctttc ccggcgctaa tagccctgga
      841 cccgtcgctg acctgtatgg acccgcctcc caggacagcg gcgtgggaaa ctatatttcc
      901 gccgctagcc cccaacccgg atccggattc ggccatggca ttgccggccc cctgatcgct
      961 accgccttca ccaatggcta ccactattcc cccatggtgg tgccccagaa gaacgtcccc
     1021 ctccccattg acgacaaaag cagcagcatg gagctccccc agatccacgc tgacacaatt
     1081 ggcaggctgg tgctggaaaa attcatggag gtgatcaaag gacaagaggc ctacagcagg
     1141 aggaaagtgc tggctggcat tgtgatgacc gagaatatga acttctgcga ggccaaggtg
     1201 atcagcgtgt ccaccggcac caaatgcgtc tccggcgaac acatgagcgt caacggcgcc
     1261 gtgctgaatg attcccacgc cgagattgtc tccagaaggt gcctcctcaa gtacctctac
     1321 gcccagctgg acctgcagtg caaccaagct accgcctacc agagcatctt cgtcagaaac
     1381 accgatggcc agtaccccta taagctgaag agcggagtgc acttccacct ctatatcaat
     1441 accgctcctt gcggcgacgc taggatcttc agccctcacg agaacgacac aggcgtggac
     1501 aagcatccca acaggaaagc caggggccag ctgaggacca agatcgagag cggccaggga
     1561 accatccccg tgaagagcag cgatggcatc cagacctggg atggagtcct ccagggccag
     1621 agactgctga ccatgtcctg tagcgacaag atcgccaggt ggaatatcgt gggcattcaa
     1681 ggctccctcc tgtccagcat cattgaaccc gtgtacctgc actccatcgt gctgggctcc
     1741 ctgctgcacc ccgaacacat gtatagggct gtgtgcggca gaattgagaa gagcatccag
     1801 ggcctccctc ccccctacca cctcaacaaa cccaggctgg ccctcgtcac cagcgccgag
     1861 cccagaaacc aggccaaagc ccccaacttc ggcatcaact ggaccatcgg agataccgag
     1921 ctggaggtgg tgaacagcct caccggaagg accattggcg gccaggtgtc caggatcaca
     1981 aagcaggcct tcttcgtgaa atacggcttc ctcatggcca acctgcctgg catcctggtg
     2041 agaaaagtca ccacagatta cggccagaca aaagccaacg tgaaggacta ccagattgcc
     2101 aagctggaac tgttcagcgc tttcaagaga gaagacctgg gcagctggct gaagaaaccc
     2161 atcgaacagg atgagttcgg actggctgag gactacaaag acgatgacga caaggattat
     2221 aaagacgacg acgacaagtg a
//
