LOCUS       sequence-324132-        1260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the Hyperactive catalytic domain of Drosophila ADAR and 
            linker at the 5' end of this domain, in the MSCV-IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Nguyen DTT, Lu Y, Chu KL, Yang X, Park SM, Choo ZN, Chin CR, Prieto 
            C, Schurer A, Barin E, Savino AM, Gourkanti S, Patel P, Vu LP, 
            Leslie CS, Kharas MG
  TITLE     HyperTRIBE uncovers increased MUSASHI-2 RNA binding activity and 
            differential regulation in leukemic stem cells.
  JOURNAL   Nat Commun. 2020 Apr 24;11(1):2026. doi: 10.1038/s41467-020-15814-8.
  PUBMED    32332729
REFERENCE   2  (bases 1 to 1260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-24"; volume: "11(1):2026. doi"; pages: " 
            10.1038/s41467-020-15814-8"
COMMENT     Sequence Label: HyperADAR control
FEATURES             Location/Qualifiers
     source          1..1260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1210..1257
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 atgtattccc ccatggtggt gccccagaag aacgtccccc tccccattga cgacaaaagc
       61 agcagcatgg agctccccca gatccacgct gacacaattg gcaggctggt gctggaaaaa
      121 ttcatggagg tgatcaaagg acaagaggcc tacagcagga ggaaagtgct ggctggcatt
      181 gtgatgaccg agaatatgaa cttctgcgag gccaaggtga tcagcgtgtc caccggcacc
      241 aaatgcgtct ccggcgaaca catgagcgtc aacggcgccg tgctgaatga ttcccacgcc
      301 gagattgtct ccagaaggtg cctcctcaag tacctctacg cccagctgga cctgcagtgc
      361 aaccaagcta ccgcctacca gagcatcttc gtcagaaaca ccgatggcca gtacccctat
      421 aagctgaaga gcggagtgca cttccacctc tatatcaata ccgctccttg cggcgacgct
      481 aggatcttca gccctcacga gaacgacaca ggcgtggaca agcatcccaa caggaaagcc
      541 aggggccagc tgaggaccaa gatcgagagc ggccagggaa ccatccccgt gaagagcagc
      601 gatggcatcc agacctggga tggagtcctc cagggccaga gactgctgac catgtcctgt
      661 agcgacaaga tcgccaggtg gaatatcgtg ggcattcaag gctccctcct gtccagcatc
      721 attgaacccg tgtacctgca ctccatcgtg ctgggctccc tgctgcaccc cgaacacatg
      781 tatagggctg tgtgcggcag aattgagaag agcatccagg gcctccctcc cccctaccac
      841 ctcaacaaac ccaggctggc cctcgtcacc agcgccgagc ccagaaacca ggccaaagcc
      901 cccaacttcg gcatcaactg gaccatcgga gataccgagc tggaggtggt gaacagcctc
      961 accggaagga ccattggcgg ccaggtgtcc aggatcacaa agcaggcctt cttcgtgaaa
     1021 tacggcttcc tcatggccaa cctgcctggc atcctggtga gaaaagtcac cacagattac
     1081 ggccagacaa aagccaacgt gaaggactac cagattgcca agctggaact gttcagcgct
     1141 ttcaagagag aagacctggg cagctggctg aagaaaccca tcgaacagga tgagttcgga
     1201 ctggctgagg actacaaaga cgatgacgac aaggattata aagacgacga cgacaagtga
//
