LOCUS       sequence-32414-e         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(138..171)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(195..228)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(458..659,660..690)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
       61 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
      121 cactggaact agaattcata acttcgtata gcatacatta tacgaagtta tggatctggc
      181 cgcgctcttc cgctgaagtt cctatacttt ctagagaata ggaacttcct cgacggtagc
      241 ggtccggttg ttttcgtgct catcgcgagt acgtatttat ttttaaaaaa taataaacgc
      301 ggcaaacaca atcacacaaa tgtgcaggca cagtgggaca aagtgcgcta aatttggata
      361 tgtaataaat ccaaaaccaa aaagaaaaca aaaaacggta aaatatttta ctgttgttaa
      421 aattcgatca ttcattattc gctgcatgaa ttagctttgc gtactcgcaa attattaaaa
      481 ataaaacttt aaaaataatt tcgtctaatt aatattatga gttaattcaa accccacgga
      541 catgctaagg gttaatcaac aatcatatcg ctgtctcact cagactcaat acgacactca
      601 gaatactatt cctttcactc gcacttattg caagcatacg ttaagtggat gtctcttgcc
      661 gacgggacca ccttatgtta tttcatcatg gtctggccat tctcatcgtg agcttccggg
      721 tgctcgcata tctggctcta agacttcggg cccgacgcaa ggagtagccg acatatatcc
      781 gaaataactg cttgtttttt
//
