LOCUS       sequence-324146-        9229 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of Flag-Polß(WT) protein with puromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Fang Q, Inanc B, Schamus S, Wang XH, Wei L, Brown AR, Svilar D, 
            Sugrue KF, Goellner EM, Zeng X, Yates NA, Lan L, Vens C, Sobol RW
  TITLE     HSP90 regulates DNA repair via the interaction between XRCC1 and DNA
            polymerase beta.
  JOURNAL   Nat Commun. 2014 Nov 26;5:5513. doi: 10.1038/ncomms6513.
  PUBMED    25423885
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-11-26"; volume: "5:5513. doi"; pages: " 
            10.1038/ncomms6513"
COMMENT     Sequence Label: pLVX-Flag-Polß(WT)-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    320..344
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             368..391
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(1419..1443)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(1475..2049,2050..2052,2053..2061)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1642..1659)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1869..1888
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2081..2680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2081..2100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2577..2597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2694..3282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2747..2767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3165..3176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3489..4122
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4250..4266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4250..4266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4263..4285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4274..4290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4298..4304,4305..4322,4323..4328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4343..4364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4481..4498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4652..5240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4732..4751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5411..6202,6203..6271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6034..6053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6272..6376)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6424..6558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6461..6480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6515..6534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6726..7359
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7406..7531
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8028..8261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8446..8490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8753..8868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        8926..9229
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagatcaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
      361 caccatggac tacaaagacg atgacgataa aggcatgagc aaacggaagg cgccgcagga
      421 gactctcaac gggggaatca ccgacatgct cacagaactc gcaaactttg agaagaacgt
      481 gagccaagct atccacaagt acaatgctta cagaaaagca gcatctgtta tagcaaaata
      541 cccacacaaa ataaagagtg gagctgaagc taagaaattg cctggagtag gaacaaaaat
      601 tgctgaaaag attgatgagt ttttagcaac tggaaaatta cgtaaactgg aaaagattcg
      661 gcaggatgat acgagttcat ccatcaattt cctgactcga gttagtggca ttggtccatc
      721 tgctgcaagg aagtttgtag atgaaggaat taaaacacta gaagatctca gaaaaaatga
      781 agataaattg aaccatcatc agcgaattgg gctgaaatat tttggggact ttgaaaaaag
      841 aattcctcgt gaagagatgt tacaaatgca agatattgta ctaaatgaag ttaaaaaagt
      901 ggattctgaa tacattgcta cagtctgtgg cagtttcaga agaggtgcag agtccagtgg
      961 tgacatggat gttctcctga cccatcccag cttcacttca gaatcaacca aacagccaaa
     1021 actgttacat caggttgtgg agcagttaca aaaggttcat tttatcacag ataccctgtc
     1081 aaagggtgag acaaagttca tgggtgtttg ccagcttccc agtaaaaatg atgaaaaaga
     1141 atatccacac agaagaattg atatcaggtt gatacccaaa gatcagtatt actgtggtgt
     1201 tctctatttc actgggagtg atattttcaa taagaatatg agggctcatg ccctagaaaa
     1261 gggtttcaca atcaatgagt acaccatccg tcccttggga gtcactggag ttgcaggaga
     1321 acccctgcca gtggatagtg aaaaagacat ctttgattac atccagtgga aataccggga
     1381 acccaaggac cggagcgaat gaaagggtgg gcgcgccgac ccagctttct tgtacaaagt
     1441 ggttgatcta gttctagagc ggccgcggat cccgcccctc tccctccccc ccccctaacg
     1501 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     1561 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     1621 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     1681 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     1741 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     1801 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     1861 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     1921 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     1981 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     2041 gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt acaagcccac
     2101 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt
     2161 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac
     2221 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     2281 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     2341 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     2401 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     2461 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     2521 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     2581 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     2641 accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg aacaatcaac
     2701 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2761 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2821 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2881 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2941 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     3001 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     3061 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     3121 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     3181 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3241 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt aattctgcag
     3301 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     3361 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     3421 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3481 gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     3541 ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc caggggtcag
     3601 atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga
     3661 agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga
     3721 tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt
     3781 ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg
     3841 actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc
     3901 cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc
     3961 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4021 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4081 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     4141 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggccttgac
     4201 attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     4261 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4321 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4381 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4441 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4501 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4561 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4621 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4681 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4741 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4801 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4861 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4921 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4981 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5041 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5101 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5161 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5221 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5281 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5341 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5401 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5461 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5521 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5581 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5641 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5701 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5761 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5821 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5881 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5941 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6001 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6061 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6301 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6361 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     6421 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6481 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6541 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     6601 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     6661 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     6721 gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta
     6781 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata
     6841 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga
     6901 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga
     6961 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc
     7021 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact
     7081 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct
     7141 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga
     7201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7501 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7561 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7621 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7681 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7741 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7801 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7861 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     7921 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     7981 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8041 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8101 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8161 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8221 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8281 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8341 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8401 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8461 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     8521 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     8581 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     8641 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     8701 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg cctttaaaag
     8761 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8821 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8881 cagggacagc agagatccag tttatcgata agcttgggag ttccgcgtta cataacttac
     8941 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     9001 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     9061 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     9121 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     9181 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatg
//
