LOCUS       sequence-324155-        6557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transfer vector for multigene expression to generate recombinant 
            baculoviruses by homologous recombination. Contains a p10/pH dual 
            expression cassette with a C-ter C-Myc tag under the pH promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Kolesnikova O, Zachayus A, Pichard S, Osz J, Rochel N, Rossolillo P,
            Kolb-Cheynel I, Troffer-Charlier N, Compe E, Bensaude O, Berger I, 
            Poterszman A
  TITLE     HR-Bac, a toolbox based on homologous recombination for expression, 
            screening and production of multiprotein complexes using the 
            baculovirus expression system.
  JOURNAL   Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.1038/s41598-021-04715-5.
  PUBMED    35132103
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-02-7"; volume: "12(1):2030. doi"; pages: " 
            10.1038/s41598-021-04715-5"
COMMENT     Sequence Label: pAC8_MF-PH-c-Myc-Cter (VE5632)
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_recomb     882..1709
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             882..1006
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1044..1649)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     protein_bind    complement(1716..1749)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1939..1987
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     2012..2031
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(2123..2232)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        2277..2368
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2293..2310
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             2483..2512
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    2658..2792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2695..2714)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2749..2768
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_recomb     2911..4268
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     CDS             complement(2927..4268)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      4415..4870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4502..4521)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4712..4733
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4887..4906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5006..5028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5066..5084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5152..5256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5257..5325,5326..6117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5475..5494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6288..6557,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagaacgg ctccgcccac tattaatgaa attaaaaatt ccaattttaa
      601 aaaacgcagc aagagaaaca tttgtatgaa agaatgcgta gaaggaaaga aaaatgtcgt
      661 cgacatgctg aacaacaaga ttaatatgcc tccgtgtata aaaaaaatat tgaacgattt
      721 gaaagaaaac aatgtaccgc gcggcggtat gtacaggaag aggtttatac taaactgtta
      781 cattgcaaac gtggtttcgt gtgccaagtg tgaaaaccga tgtttaatca aggctctgac
      841 gcatttctac aaccacgact ccaagtgtgt gggtgaagtc atgcatcttt taatcaaatc
      901 ccaagatgtg tataaaccac caaactgcca aaaaatgaaa actgtcgaca agctctgtcc
      961 gtttgctggc aactgcaagg gtctcaatcc tatttgtaat tattgaataa taaaacaatt
     1021 ataaatgtca aatttgtttt ttattaacga tacaaaccaa acgcaacaag aacatttgta
     1081 gtattatcta taattgaaaa cgcgtagtta taatcgctga ggtaatattt aaaatcattt
     1141 tcaaatgatt cacagttaat ttgcgacaat ataattttat tttcacataa actagacgcc
     1201 ttgtcgtctt cttcttcgta ttccttctct ttttcatttt tctcctcata aaaattaaca
     1261 tagttattat cgtatccata tatgtatcta tcgtatagag taaatttttt gttgtcataa
     1321 atatatatgt cttttttaat ggggtgtata gtaccgctgc gcatagtttt tctgtaattt
     1381 acaacagtgc tattttctgg tagttcttcg gagtgtgttg ctttaattat taaatttata
     1441 taatcaatga atttgggatc gtcggttttg tacaatatgt tgccggcata gtacgcagct
     1501 tcttctagtt caattacacc attttttagc agcaccggat taacataact ttccaaaatg
     1561 ttgtacgaac cgttaaacaa aaacagttca cctccctttt ctatactatt gtctgcgagc
     1621 agttgtttgt tgttaaaaat aacagccatt gtaatgagac gcacaaacta atatcacaaa
     1681 ctggaaatgt ctatcaatat atagttgctg atatcataac ttcgtatagc atacattata
     1741 cgaagttatc tggtttaaac gtacccgtag tggctatggc agggcttgcc gccccgacgt
     1801 tggctgcgag ccctgggcct tcacccgaac ttgggggttg gggtggggaa aaggaagaaa
     1861 cgcgggcgta ttggtcccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg
     1921 accgaacccc gcgtttatga acaaacgacc caacacccgt gcgttttatt ctgtcttttt
     1981 attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc agttagcctc
     2041 ccccatctcc cggtaccgca tgctatgcat cagctgctag caccatggct cgagatcccg
     2101 ggtgatcaag tcttcgtcga gtgattgtaa ataaaatgta atttacagta tagtatttta
     2161 attaatatac aaatgatttg ataataattc ttatttaact ataatatatt gtgttgggtt
     2221 gaattaaagg tccgtatact agtatcgatt cgcgacctac tccggaatat taatagatca
     2281 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
     2341 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
     2401 gcgcggatcc cggtccgaag cgcgcggaat tcaaaggcct acgtcgacga gctcactagt
     2461 cgcggccgct ttcgaatcta gagaacaaaa actcatctca gaagaggatc tgtaactgca
     2521 gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     2581 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     2641 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     2701 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2761 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg
     2821 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     2881 tcataacccc taggcgatta attgatccgg gttattagta catttattaa gcgctagatt
     2941 ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca ttaaatttat
     3001 aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct ttatatctga
     3061 atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt tcaaacaagg
     3121 gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac gccacaaaac
     3181 ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt ttgttttgta
     3241 ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca tcactgtcgt
     3301 tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ttggacatat ttaacatcgg
     3361 gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg ttagaagttg
     3421 cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg gctaacacgt
     3481 ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg cgtttttggg
     3541 cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa agcgatggtg
     3601 caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt ggagctgatg
     3661 ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga ggcggaggtg
     3721 gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac acagtcggca
     3781 cctcaactat tgtactggtt tcgggcgccg tttttggttt gaccggtctg agacgagtgc
     3841 gatttttttc gtttctaata gcttccaaca attgttgtct gtcgtctaaa ggtgcagcgg
     3901 gttgaggttc cgtcggcatt ggtggagcgg gcggcaattc agacatcgat ggtggtggtg
     3961 gtggtggagg cgctggaatg ttaggcacgg gagaaggtgg tggcggcggt gccgccggta
     4021 taatttgttc tggtttagtt tgttcgcgca cgattgtggg caccggcgca ggcgccgctg
     4081 gctgcacaac ggaaggtcgt ctgcttcgag gcagcgcttg gggtggtggc aattcaatat
     4141 tataattgga atacaaatcg taaaaatctg ctataagcat tgtaatttcg ctatcgttta
     4201 ccgtgccgat atttaacaac cgctcaatgt aagcaattgt attgtaaaga gattgtctca
     4261 agctcggatc gatcccgcac gccgataaca agccttttca tttttactac agcattgtag
     4321 tggcgagaca cttcgctgtc gtcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4381 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4441 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4501 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4561 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4621 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4681 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     4741 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     4801 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4861 tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     4921 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4981 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     5041 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     5101 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     5161 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5221 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5281 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5341 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5401 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5461 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5521 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5581 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5641 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5701 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5761 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5821 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5881 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5941 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6001 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6061 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6541 aagtcgtgtc ttaccgg
//
