LOCUS       sequence-32416-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..90)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(74..90)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(127..160)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(345..363)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gtgcacgttt gcttgttgag aggaaaggtt gtgtgcggac gaattttttt ttgaaaacat
       61 taacccttac gtggatccac tagttctaga gcggccgctc gagttaatta agcatgccta
      121 ggctagataa cttcgtatag catacattat acgaagttat agcttctgat ggaattagaa
      181 cttggcaaaa caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct
      241 tcaggctatg aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc
      301 aagagaaaaa caatgatccc acgagagatc gaattctagt atgtatgtaa gttaataaaa
      361 cccatttttg cggaaagtag ataaaaaaaa catttttttt ttttactgca ctggatatca
      421 ttgaacttat ctgatcagtt ttaaatttac ttcgatccaa gggtatttga tgtaccaggt
      481 tctttcgatt acctctcact caaaatgaca ttccactcaa agtcagcgct gtttgcctcc
      541 ttctctgtcc acagaaatat cgccgtctct ttcgccgctg cgtccgctat ctctttcgcc
      601 accgtttgta gcgttacgta gcgtcaatgt ccgccttcag ttgcattttg tcagcggttt
      661 cgtgacgaag ctccaagcgg tttacgccat caattaaaca caaagtgctg tgccaaaact
      721 cctctcgctt cttatttttg tttgtttttt gagtgattgg ggtggtgatt ggttttgggt
      781 gggtaagcag gggaaagtgt gaaaaatccc ggcaatgggc caagaggatc aggagctatt
      841 aattcgcgga ggcagcaaac acccatctgc cgagcatctg aacaatgtga gtagtacatg
      901 tgcatacatc ttaagttcac ttgatctata ggaactgc
//
