LOCUS       sequence-32417-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4AD
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     171..189
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    374..407
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aacagcgctg actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt
       61 acatcaaata cccttggatc gaagtaaatt taaaactgat cagataagtt caatgatatc
      121 cagtgcagta aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat gggttttatt
      181 aacttacata catactagaa ttcgatctct cgtgggatca ttgtttttct cttgattccc
      241 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
      301 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
      361 tccatcagaa gctataactt cgtataatgt atgctatacg aagttatcta gcctaggcat
      421 gcttaattaa gctagtggat ctaaacgagt ttttaagcaa actcactccc tgacaataaa
      481 aacgcaatta tctttaaata aatatattct atttattaac caagtagcta atatagctaa
      541 aatcaatttg ttgctaactt aaaactaaca taattatgta ttagttattt acattctttt
      601 ggttggttac aaccaaataa ataatatcac ttttaaaaac ttaagccagg aactgaataa
      661 ttttctattt ggctttagtc gacggtatcg atctagaggc gcgccttact tgccgccgcc
      721 cagggggccg tagcgggtgc ggttctggct cacgcggttg cgcaggcgct gcacgcgctg
      781 gcgcagctcg gccacgcggg tctccagggc ggtgttctcg cgctccagga aggcggcctc
      841 gatctccaga ctagtacctc cacctccacc tccaccacct cctccag
//
