LOCUS       sequence-324181-        8734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses BICD2 in mammalian cells with a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, 
            Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, 
            Cookson MR
  TITLE     LRRK2 mediates tubulation and vesicle sorting from lysosomes.
  JOURNAL   Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 
            10.1126/sciadv.abb2454. Print 2020 Nov.
  PUBMED    33177079
REFERENCE   2  (bases 1 to 8734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-11-11"; volume: "6(46). pii: 6/46/eabb2454. doi"; pages:
            " 10.1126/sciadv.abb2454. Print 2020 Nov"
COMMENT     Sequence Label: GFP-BICD2
FEATURES             Location/Qualifiers
     source          1..8734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(905..907,908..910,911..1621)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(938..966)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1561..1581
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4269..4293)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4325..4342)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4331..4555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4601..5029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4688..4707)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4898..4919
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5038..5058)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5043..5372
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5223..5358
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5285..5304
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5439..6233
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5493..5512)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6103..6122
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6407..6528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6444..6463)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6498..6517
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6577..6593)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6577..6593)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6590..6612)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6601..6617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6625..6631,6632..6649,6650..6655))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6670..6691
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6808..6825)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6979..7567)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7059..7078)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7738..8529,8530..8598))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8361..8380
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8599..8703)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa gcaggcttga aggaattcgg
     1681 taccatgtcg gcgccgtcgg aggaggagga gtacgcgcgg ctggtgatgg aggcgcagcc
     1741 ggagtggctg cgcgccgagg tgaagcggct gtcccacgag ctggccgaga ccacgcgtga
     1801 gaagatccag gcggccgagt acgggctggc ggtgctcgag gagaagcacc agctcaagct
     1861 gcagttcgag gagctcgagg tggactatga ggctatccgc agcgagatgg agcagctcaa
     1921 ggaggccttt ggacaagcac acacaaacca caagaaggtg gctgctgacg gagagagccg
     1981 ggaggagagc ctgatccagg agtcggcctc caaggagcag tactacgtgc ggaaggtgct
     2041 agagctgcag acggagctga agcagttgcg caatgtcctc accaacacgc agtcggagaa
     2101 tgagcgcctg gcctctgtgg cccaggagct gaaggagatc aaccagaatg tggagatcca
     2161 gcgtggccgc ctgcgggatg acatcaagga gtacaaattc cgggaagctc gtctgctgca
     2221 ggactactcg gaactggagg aggagaacat cagcctgcag aagcaagtgt ctgtgctcag
     2281 acagaaccag gtggagtttg agggcctcaa gcatgagatc aagcgtctgg aggaggagac
     2341 cgagtacctc aacagccagc tggaggatgc catccgcctc aaggagatct cagagcggca
     2401 gctggaggag gcgctggaga ccctgaagac ggagcgcgaa cagaagaaca gcctgcgcaa
     2461 ggagctgtca cactacatga gcatcaatga ctccttctac accagccacc tgcatgtctc
     2521 gctggatggc ctcaagttca gtgacgatgc tgccgagccc aacaacgatg ccgaggccct
     2581 ggtcaatggc tttgagcacg gcggcctggc caagctgcca ctggacaaca agacctccac
     2641 gcccaagaag gagggcctcg caccgccctc ccccagcctc gtctccgacc tactcagtga
     2701 gctcaacatc tctgagatcc agaagctgaa gcagcagctg atgcagatgg agcgggaaaa
     2761 ggcgggcctg ctggcaacgc tgcaggacac acagaagcag ctggagcaca cgcggggctc
     2821 cctgtcagaa cagcaggaga aggtgacccg cctcacagag aatctgagtg ccctgcggcg
     2881 cctgcaggcc agcaaggagc ggcagacagc cctggacaac gagaaggacc gtgacagcca
     2941 tgaggatggg gactactacg aggtggacat caacgggcct gagatcttgg cctgcaagta
     3001 ccatgtggct gtggctgagg ctggcgagct ccgcgagcag ctcaaggcac tgcgcagcac
     3061 gcacgaggct cgtgaggccc agcacgccga ggagaagggc cgctatgagg ctgagggcca
     3121 ggcactcacg gagaaggtct ccctgctaga gaaggccagc cgccaggacc gcgagctgct
     3181 ggcccggctg gagaaggagc taaagaaggt gagcgacgtc gccggcgaga cacagggcag
     3241 cctgagtgtg gcccaggatg agctggtgac cttcagtgag gagctggcca atctctacca
     3301 ccacgtgtgc atgtgcaaca atgagacacc caaccgtgtc atgctggact actaccgcga
     3361 gggccagggc ggggccggcc gcaccagtcc cgggggccgc accagccccg aggcgcgtgg
     3421 ccggcgctca cccatcctcc tacccaaggg gctgctggct cctgaggcgg gccgagcaga
     3481 tggtgggacg ggggacagca gcccctcgcc tggctcctca ctgccatcac ccctgagtga
     3541 cccacgccgg gagcccatga acatctacaa cctgatcgct atcatccgtg accagatcaa
     3601 gcacctgcag gcagccgtgg accgcaccac ggagctgtca cgccagcgca ttgcctctca
     3661 ggagctgggc cccgccgtgg acaaggacaa ggaagcgctt atggaggaga tcctcaagct
     3721 gaagtcgctg ctcagcacca agcgggagca gatcaccacg ctgcgcactg tgctcaaggc
     3781 caacaagcag acggccgagg tggcccttgc caacctgaag agcaagtatg agaatgagaa
     3841 ggccatggtt accgagacca tgatgaagct gcgcaatgag ctcaaggccc tcaaggagga
     3901 cgcagccacc ttctcctcgc tgcgtgctat gtttgccacc aggtgtgacg agtacattac
     3961 acagctggat gagatgcagc ggcagctggc ggctgctgag gacgagaaga agacgctgaa
     4021 ctcgctgctg cgcatggcca tccagcagaa gctggcgctg acccagcggc tggagctgct
     4081 cgagctggac catgagcaga cccggcgtgg ccgtgccaaa gccgccccga agaccaagcc
     4141 agccacaccg agcgtaagtc acacctgtgc ctgtgccagc gacagggccg agggcaccgg
     4201 gctggccaac caggtgttct gcagcgagaa gcacagcatt tactgtgatt agctcgagtg
     4261 cggccgcaac ccagctttct tgtacaaagt ggtgataatt aattaagata aacccgctga
     4321 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     4381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     4441 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     4501 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     4561 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     4621 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4681 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4741 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4801 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4861 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4921 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4981 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     5041 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5101 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5161 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5221 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5281 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     5341 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     5401 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     5461 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     5521 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     5581 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     5641 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     5701 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     5761 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     5821 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     5881 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     5941 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6001 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6061 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6121 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6181 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6241 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     6301 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     6361 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     6421 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6481 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     6541 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     6601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     7381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     7441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     7501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     7561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     8341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     8401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     8461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     8521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     8581 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8641 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8701 gcgcacattt ccccgaaaag tgccacctga cgtc
//
