LOCUS       sequence-324185-       14299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second CCM operon lacking acRAF; Deletion of putative rubisco 
            chaperone, acRAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 14299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCCM' Delta-acRAF
FEATURES             Location/Qualifiers
     source          1..14299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(410..504)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(525..1184)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1099..1118
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1185..1287)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1353..1371
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(1392..1410,1411..1416,1417..1433,1434..1439,
                     1440..1465)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    1392..1410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1417..1435
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1475..1486
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      12870..12956
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(13129..13752)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     13660..13679
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(13753..13857)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(14033..14299,1..322))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14113..14132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgact agtgcttgga ttctcaccaa
      421 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
      481 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
      541 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
      601 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
      661 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
      721 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
      781 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
      841 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
      901 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
      961 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     1021 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     1081 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     1141 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     1201 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     1261 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccagatatc gacgtctaag
     1321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     1381 ttcacctcga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg
     1441 agcacatcag caggacgcac tgaccgaatt cattaaagag gagaaaggta ccatggccag
     1501 caacaaagcc tttacattgg ctgtcgccaa cctcaaaggt ggctgtggca agacgaccat
     1561 cagcacaaac atcagtgcgg gcttgacgca gcgtggtcgt gttggtctgg tcgatgccga
     1621 cccccaaggc gcactcaagc attgggttga ctggggaagc aaagaggcgg acgctcaagt
     1681 ccccgtctta tactcagatc acaccgatcc cgttcaaaat cttaaattgg cgcaaccaaa
     1741 ccatgacttt gtcgtcgtgg attgcccgcc ttccctagac atggcaatta cctgtcaatt
     1801 gatgattgag tgcgatttta tattaattcc tgtactgccc tcccctttag atctttgggc
     1861 gagcactcaa acaatcgaga tgattgaaag cgcacgcaaa accaacccca agctaaaagc
     1921 tgcgttggta ctcaatcaaa cagagccgcg cagtgcaatg acacgagcaa tgcaaacaac
     1981 cattgagcga ctaggggttc ccgtactcac tacctctgtg cgacgacgcg ctgtgtaccg
     2041 aaatgccgta gtggaaggcg ttagcgtttt ccagctcgga gcaagaggcc gctcagcggc
     2101 tgatgaaatc aatcaaatcc ttaacgaggt tatcccgtca tgactaattt agaagataaa
     2161 ctgagtgcaa gcatcaagac cgagaacaag gacacgatag cgcctgcgtc aacgccagcc
     2221 aaaccagccg cgcgctcggc tgcacccgaa accaccaagc ctgcatcgac aggggccaaa
     2281 cctgccacac ggacggctgc gaaaaagcct gtagcgcgtg cgacgacaac aaaacctgct
     2341 gtatcaaaat ccagcaagcc atcggctgat gatattcagg ttccggcaca cgacacatcg
     2401 acgcatcctc gccgtgtatg gccagattga ttttgtccga acatcgcgaa actaacgtca
     2461 aactatgaac ccagcgactg aagaatatca ggctcttttt gaagacttgg gcgaaccgat
     2521 cgctgaagtc cttgagaccc attggattga gctttcgcga ttgttcagcc cgatgggttt
     2581 caaggctttt ctagaaagcg tttccaatct taaggctttg ggacgcggtg atgagttagt
     2641 tatctcgttt attgaatcgg caggtcctgt tacaaggtca attggtgaaa cagcagtttt
     2701 cgaactgctg tcttccgtta ttaaaatgtt ctccaaggcc agtgctgaag ttttagcaat
     2761 ggttttcaaa tcggcacctg ttgctgtcag tcggctcggt gaggctgaac tgttcgttgc
     2821 ctatctttct ttactggatt acatagcggc caaagccccc cgagccttac gtccacttct
     2881 tggtcaaata gagcaactgc ttggtgtgct gacgcttggg ggattgcggc gttggatcac
     2941 ttgggggatt caagcgcacc gaaccgattt tgccgcccaa gaggcctact ttagtcttgc
     3001 aacgcctgaa tcccgtgctg tgttacagcg cgagcgaagg ggtgtattgt ttgttgatgt
     3061 acaacgccgc ttggtgatgt atttacgcgc actgtggggg cgtgattttt ttctacgccc
     3121 cacttcgggt gatttcgaaa gccgtgcggg ataccgtccg ttcattgatg aactatttat
     3181 caatttaccc gacgcgtatg atgattacgc cgttgaaggc gaacaaacag ttgatggtct
     3241 gactgtctat cgtgcggcag cggctcatgc cgcagcacat caaatttatt cgcgtctcga
     3301 ctccgatgcg aagaacgcat ccccactgga ccgcgcgacc attggtttat ttgaagacgc
     3361 gcgtgtcgaa aaactcgcaa tggccgattt ccctggattg gttcccctgt ggaggaatct
     3421 caaccaactg acggttgctg ccctccccgc tgacgcacga acaggagaga atccaactct
     3481 tggcgcactt ttcgaccgga tagcgctggc actatgcgat gagaattcat cggataccca
     3541 cccgcttgtc gaatggtcgc gtgaggcgtt taaagcagca cttgatcgtc tggaagatca
     3601 ggagatggcg cgagaactgg gcatcgctct agccgaaaag atccagcagc aaaacctggt
     3661 atttcaaagc cgtaccgata tcccaagtag cccgtaccgt gatgacaata cctatttgtg
     3721 gcaaacaccg gaaacaggca agagcaatgc acataccgcg gccagcattc aggaaaagcg
     3781 caagtacgtt agcgtgatgc agatgctgca cgccttggat gtggagtacg ccacggcgga
     3841 aagcgcggat gaaatatggg tgcttgccac cgaacttttt gatgacgatg gcacgacgtt
     3901 caatgagctt tatggcacgc caccgactgc ttcgcctcga tattatcccg aatgggatta
     3961 ccaatcacag cttgagcgac cattgtggac gaccctgatg gagaagaaac ccaaattggg
     4021 cgatcctgcg gtggttgata atattttgtt cgagcacgcc ccaatggtac ggcgtttgaa
     4081 gtacctgatc gaaggcttac agccacaagg tgtcgtgcga cagaaaaaaa tcgaggatgg
     4141 tgatgaagtc gatctcaatg cagccattgg cgcaatgatc gatattcgaa tgaatcgtca
     4201 accggacccg cgcattggca ttcgtcgacg tatccatgtg cgtgatctat ccgtgatttt
     4261 gctcattgat ctctcagaat caaccaatga tctcgtgcaa accggtgaag aagaaaaaac
     4321 cgtacttgat atggctagag aggcagcagc attactcggt gatgcattga atcgcattgg
     4381 tgatccctac gctattcatg gttttgattc caatgggcgc agcgatgttg agtatttccg
     4441 cttcaaagac tttgatgacg ccttcgatga cctcgccaag gctcgtctgg ccggaatgac
     4501 ggggcaactc tccacacgca tgggcactgc cttgcgacac gcgggccatt tgttggattc
     4561 acggcccagc cagaagaaac ttattctgtt acttaccgat ggcgagcctt ccgataatga
     4621 tgtgcgcgac ccccaatatc tacgccaaga caccaagcgc gttgttgagg aattagggaa
     4681 acggggcatc aatacgttct gcgtcaccct cgatccaaga gccgatgatt atgtcgaacg
     4741 aatttttggc ccgaaacact acatgatcct cgaccatgta gctcgactgc ctgagaaact
     4801 accggcactt tatttgtcga tgacgcgata gaactttccg tccgagcttc agacaagaaa
     4861 caaatctcat gaagctgttc aaattgctgc tcgggatacg aggacaatgc gcctgaaacc
     4921 cgattaattt taatttgcgt atacgcaaga taactacccg gccaaggccg gtctgtgatg
     4981 gagaaagcgc atgatgaacc tgcaatggtt aattccattg ttacccttac tgtccgccct
     5041 actggtgcaa ttgtttgccg ctcgactcgg taaacgtgtt gcccacctga gcgtggcgtt
     5101 gggaacactg accgtcattg ttgccgcata tcagctcgta gcgtatatcg gtaccgatgc
     5161 gagcccaagc tggcattctc tgggcacgat gtggggcagt ctgtacgtcg atccgctgag
     5221 cagcatcatg tcgctggttg tagcgggcat cagcctgatt gttcatgtct attcaatccg
     5281 ctacatgacc gaagaaccgg gctatccccg gttcttcttg ctgctggatt tgatgaccgc
     5341 ttccattttg cttatggtcg ccgccggtga cttggtcaca ttgctgatcg cctggcacat
     5401 gatcggcatc atgctctact ttcttttggg tcagaataca gaatcatggc cgagccagcg
     5461 ctacgccttt tggacgttta tcacgtaccg gttgggggac ttgccattgg tgctcgccgc
     5521 cgtgctgctt tatcaaacct atggtgcgat tgacttcccc accttatttt cccgtatcgc
     5581 ggccgatcca aacgccacca tcatggggct gcccacggcg ataacggctg cttttttggt
     5641 ggcgctctct gcctttgcaa aatccgccca attcccgcta catacctggc tcccctatac
     5701 catggagggc ccgacgccgg tttcagcttt gatgcatgcg gggattgtca acgccggtgg
     5761 ttttatcatc aaccgcttcg cgcctgtttt tgttcacagt gatggcgtac tgcacatgct
     5821 gtttgtcgtg ggtctgatta ccgcgctggt gggatcggtg ctgatgttga cgcaaaacga
     5881 catcaaaaaa tcactgggtt attccaccat gggtcaaatg ggcttcatgg tcatggagtg
     5941 tggtcttggc gctttttctc tggccgtgtt ccatctcatt gcgcatggtc tgttcaaagg
     6001 caccatgttc ctcggttcgg gcagcatgat tcatgaagca cgcaagcacg acggcgtgcc
     6061 acataaccct ctgcacacct tcttggttga acgaaagtct gcatcattaa aattgccttg
     6121 gttatttatc ggcttggcta cgctggtcgt acctctgttc attctggtta ttgcacactg
     6181 gttcgtggca ccggattttt tcgaaaaaca aggtgccatc gtgctgctgt tcttcggctg
     6241 gattacgggg gttcaggttc tttttgccac acatcatctg gatgccaata acccaattcg
     6301 catgatgatg atgattttat tgtcgtttac cttgattgtg gtgggctaca ccttcattgg
     6361 tcatgctttc gaaaacttcc tttaccccga agaagccttc cgtaatgcgc tgtaccatgc
     6421 agcaggcatc gataaactga cgttcgacgg acttgttttc ttgctcgctt tgattgtggt
     6481 ggcaggctgg tttagcagct atttggccag tcgtgagaaa tcggttttcg gtgaccggtt
     6541 cggagcggtt cgcctcactc tctactcact gatctcgcgt gaattttatg tagccgatct
     6601 ctacgaccgg atggcgcact ggctgcttga tgcatccaaa cgcttcaatg tctggatgag
     6661 gtggtattga tatgtccttt ttcttgatgt tactggccgg gttctttctg cccttgttcc
     6721 ccttgagcat ggtattcaat gccctgctcg gccgaatcaa aaaccccatc gcgcgggtac
     6781 tgctgatttt tatctggcct caaatcggcg ttttcttgct tgcatgggct ggtttgccgc
     6841 tggattccgg attcttcgtt ggctgggcag tgcttacggc agtcttctat gcctggcgat
     6901 tgctgacggt gcgtgtgctc tcccagtggg ccgggatgct cgccacgtcc gcctgggcgc
     6961 tggcttggat tatcgccgcc gatcttccag ccagtagcga actgcacagt ttcgccctgt
     7021 ggatgtccgt tgcgccggct ttgctgatct tcattgccat tgcactgcaa gaacgttttg
     7081 gcgctgctta cagtggttta gttgtggggt tggcgcgggc tttaccgcgc ttatccagcg
     7141 tcattgtcat cgtggtgttg gccgcaatgg cactccctat tttccccagt ttttttgcaa
     7201 tgttggcatt gctggcgcat agctccatct tattcgctct gccgattttg cttacctggt
     7261 tgctttggag ttgggcaggc acgcggttat tgaatgggtt tgttttcggg ccacgcgccg
     7321 agacagatgc cgccgatctc tcttcaacca caacatgggg ctttgttggc ctgatagcaa
     7381 cgattgttgt gattaatctg atttggacag gagcttgatc ccatgagcaa actccctttg
     7441 ggtaaacgcc tgaaaattcg ttccatggtg cacatggctg ccgagccaat ccccaatttc
     7501 tggcctatgc ggacgttcat tcaccataac ccgctccatg gtctggagca tctgcccttc
     7561 gaacaggcgg ttcgccaggg tgaaaaactc tttcacgcac ggggattttt gccgcgtgag
     7621 gattaccagc gctatcacaa ggaaggccga gttgatcaaa acagcataaa gcgtgacata
     7681 gccgatttta tttcaaaaca agaaacgctc aacggtttgg atttggcatc gttacttagc
     7741 gacttgatgt gttcggttaa gaacaaagta actagaacgc gcgcgctcgc cgatcatgat
     7801 gatgtgttcc aagccttgca cgggaaacaa ctggaaaatg cagaggcgct cgatcttaaa
     7861 gcgctaactc aacgcttatg tgcgcagttt gcaccagagc gccccctgta cgaagccatc
     7921 gatttgctgt tcggcacgca aatgggcacc acactcgatg aattggtaat caaaagctgt
     7981 ctcgactttt tcgatgaagg ccaatcaacc atccaaatgc ccggccgcca ccaaggattg
     8041 ttcgcagctt ggacggccct ggcaaaacgt aatttacgcc tgtttttacg tggcatgcat
     8101 atcaaacaga tcctcgatca ggacgatacg ccagagggca tcatcgccta cattctcgac
     8161 gaactgggca ttgaggaggc tcactgggat gggctgatta cccgcgaact gactcgtttg
     8221 catggctggg caggttttat tcgctggcgc tcctcttcca agcactacta ttgggccgag
     8281 cagtacccgg gggatctcat cgatttcctg gccatccggc tcgttttagg cttggccttg
     8341 atccgtgaac atagccgtca aaagcgcaca ccgatgacag tcaaagtgct gcaagaatat
     8401 atcgaagggc acaccgccga atgctatctg cgtcaagcct attacggtgg ctgcatattg
     8461 cccgcgtttg ctcatgatgt tgatgatgcg ctgtcgcata aaaagcctca aaggatcaac
     8521 aacattcttc cgggctacct gcgccaacaa cgccaattcg aggcaacacg acaagctgat
     8581 gcgcttcgtg atttagccag caaagcgggg caaaccgatg cccttatggc gctgaatgcg
     8641 cctcaaatca agcaactcat gacacttatc gaggcgtttg aaaacgaaga aggcatgatc
     8701 tggcttcgtg cgatggaatc ggtctatcga cgggaaatca tcaaccagat tcaactgtat
     8761 gcaccgcata aaaaagaaaa acggcccttt gcccaggcat tgttctgtat cgatgtgcgc
     8821 tccgagccga tacgccgtaa tctggaaacg gtaggcgagt atcaaaccta tggtatcgcc
     8881 gggttttttg gtgttccggt aagctatatt ggccttggca agggcagtga agttaatctt
     8941 tgcccggtgg tcattacccc taaaaatctg gtgcttgaag tgcccgtggg tgccacaagc
     9001 attgaaacag acttttattc ttccgccgac catgtgctac atgagatgaa aagctcgatc
     9061 ctttcaccct acttcacggt tgaagcggcc ggtttgctgt ttggtttcga catgatcggc
     9121 aaaaccattg ccccgcgacg ctacacccaa atacgcaatc atatcgaacc aaaagcacag
     9181 gcaactcgtt tgctggtgga taaactcacc cgcgaacaag ccgactcaat cgtccgttcg
     9241 ctgcaacgcg ccatgattgt gcgcgccatt catcaggaat ttggcatcga acgcgaagca
     9301 gtcaccgatg ccatgatccg cgaactgcgc gaagcggcca tggataacta tcacgaacag
     9361 accgaattcg cgcgccgttt cgccttgagc ccaacggccg aaactcagtt tatcgcaggg
     9421 ctgaaaaagg actataaaat caatcgctca tttgtttcca tgcaaatgga acgcttggcc
     9481 aggattggtt ttagcctcga tgaacaggtg ttttatgttg acaaggcact cacatccatc
     9541 ggattgaccg aaaacttttc acgctttgtt ttattggccg gtcacggcag cacttccgac
     9601 aataatccct acgaatccgc gcttgactgc ggtgcatgtg gtggtagtca tgggctggtt
     9661 tctgcccggg tgcttgccca catggccaat aagcctgaag tacgtcgcag actggccaag
     9721 caaggcatcc agatacctga agatacttgg tttgtgtccg tcatgcacaa caccacaacc
     9781 gatcaattgt cactgcaaga ccttgatttg cttccaaaca gccatcttgt ttacctcgaa
     9841 cgcttgcgta acggcttacg tgcggccacc cgtttgtcag cggcagaacg cttgcctgct
     9901 ctgcttgatc atccttcgcc caacatcgac acattatcgg cacaaaaaca aattgagcgg
     9961 aatgccagcg actggaccca agttcggcca gagtggggct tggcgagaaa tgcgagcgtc
    10021 gtcgccggcg gccgacattt gaccgagggt gcgaacttaa gcggtcgaac gtttttgcag
    10081 tcttacgatt atcgactcga tcccaaaggc cgccaccttg aaaacattct cagcaacccg
    10141 ctaattatcg gccagtggat caatcttgag cattatttct cagcggtaga taacgaacac
    10201 tttggcagtg gcagcaaggc ctatcacaac gtcgtaggtc gttttggtgt ggttacgggt
    10261 aatttaagtg acttgcgaac agggttacca gcacagtcgg tgcttaaaga tggacgccca
    10321 taccacgagc ccatccgtct cttggcgatt atcgaagcac ccgcagcatt caccctcgaa
    10381 gtagcgggtc gattgcccaa ggtgatgtcc ctgattacca acggctggat cactgttgtt
    10441 gtcgttgatc cggaaacggg cgatcgtctt ttttatgatc gcggcgaatg gtacaatctc
    10501 aacaatgatc cgcagtacac gccctcggtc aaacccttgc ttgaagaaga actcagcgca
    10561 tgaaaaacct caaccttacc caattgaaaa aactggaaat catcctcgaa ggtgaacatc
    10621 aatcattcgc cacggattta ctcgaccgag caggcgtcaa gggctacacc attgtcggca
    10681 acctttcagg caaaggggct caaggctttc atgaaggcca tctcatgttt aacgaagatg
    10741 aagtgctgaa catgatcatc accgccgttc cggaagagct cgtttctccc ttgctggaag
    10801 gcttcgcccc ctttttcagc aaacacagcg gagtagtatt tgtttctgac attcaggtca
    10861 gtcgtccggt caagttcaaa acagacgcta aataaatacg atttaaatca gaaagacggc
    10921 tacatcgacc agaacaaggc aatttaaatg acacaaaatg cagatcaata tcgcgtacgc
    10981 aaagagccct attatcgagc tgtacaagac gaaatcgagt tatatcgcgc cggttacgaa
    11041 gcacgcattc ccatgatgct caaaggcccg accggctgtg gtaaatcacg ttttgtcgaa
    11101 catatggctt ggaagctcaa ccgcccctta atcaccatcg cgtgtaatga agacatgacg
    11161 gcatccgatc ttgtcggccg ctatttgctc gataaagatg gcacccgttg gcaagatggc
    11221 ccgcttacgg tcgctgcgcg cattggtgcc atctgctacc tcgatgaaat tgttgaggcc
    11281 cgtcaggata ccatcgtcgt catccatcca ttgaccgatc accgtcgagt actgccactt
    11341 gagaaaaagg gcgaattggt tgaagcgcat cccgatttcc aaatcgtgat ttcttacaat
    11401 ccgggctatc aaagcctgat gaaagacctc aagcaatcca ccaagcaacg ttttggtgcg
    11461 cttgattttg actaccctaa accagacatt gaagccgaaa ttgtgagcca cgaggccggg
    11521 gttgataaag acactgctga aaaactggtt cagatcgcac aaaaagcacg taacctcaag
    11581 ggtcatggcc tggatgaagg cctctccacg cgtctgttag tctatgcagg caaattgatt
    11641 gccaaaggcg ttgatgcccg cgctgcctgc accatgacgc ttgtcaaccc gatcacagat
    11701 gatgtcgaca tgcgcgatgc actcgacacc gtcgtcaaaa cgttctttta agccttccgt
    11761 accaacaggt aatacgcaca acatgaaaca caccagctat gtcggtcttg atcttgacgc
    11821 acaaggcggc atgacgcaca tcggaaaaat tatccgagat ggttggctgt ttggctttat
    11881 tccagaaacg gaaacgggta ccggctggac ccacacacag attcaaatgc tgtatgaaaa
    11941 ggtgtttgct gcctgggaac cgtatgcaca catcccgagc cgcttgccac ccgagcttaa
    12001 agaaaagtac ctaagaattc aggaagatgc actaaaacgc gcccgggaaa atggatggga
    12061 cccggaactg tccgatgatg attaaaactc cagaccaaac tgtatccgcc caaatcaaga
    12121 tacaagtgaa aatcaatgaa atcagtgtgc atcttcaggg aagatgcgca tgaacaacat
    12181 tgatttgcgc gtctattcgt ttatcgattc gcttcaaccg cagcttgcat cctatcttgc
    12241 gacatcatcg caaggctttc ttcccgttcc gggtgatgcc tgcttgtgga ttgaagtcgc
    12301 gccgggcatg gccgtccatc gcctcagtga tattgcgcta aaggccacga acgttcgtct
    12361 cggcgaacag gtagtcgagc gtgcttttgg ctcgatggaa attcattacc gaaaccaaag
    12421 cgacgttctc gcatccggtg aggccgtttt aagagaaatc aatcacgcgc aagaagatcg
    12481 tctgccttgt cgcatcgcat ggaaagagat cattcgagcg attacccccg atcatgccac
    12541 cttgatcaat cgccagttgc gtaaaggctc tatgctgttg ccgggcaaaa gcatgttcat
    12601 ccttgaaaca gaaccggcag gttatattgt tcaggctgcc aacgaggccg agaaagcagc
    12661 tcatgtgact ctgatcgatg tacgtgcttt tggtaacttt ggtcgcctga ccatgatggg
    12721 cagcgaagcg gaaaccgaag aagccatgcg ggcggctgag gccacaatcg caagcatcaa
    12781 cgcgcgtgcg cgtcgcgctg aagggttcta aaatgtaact gtattcagcg atgacgaaat
    12841 tcttagctat tgtaatactc tagaggcatc aaataaaacg aaaggctcag tcgaaagact
    12901 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
    12961 cgccgctagc agttcgtaga cggaaacaaa cgcagaatcc aagcaaaagg gaataagggc
    13021 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    13081 gggttatgtc cactgggttc gtgccttcat ccggatatcc aggaactgtt aagacccact
    13141 ttcacattta agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc
    13201 tggctctgca ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact
    13261 atcagtagta ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac
    13321 gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa
    13381 accttgttgg cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg
    13441 ccgtgtacct aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact
    13501 tttagcgtta ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt
    13561 atggtgccta tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac
    13621 atgccaatac aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa
    13681 accttcgatt ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc
    13741 taatctagac atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    13801 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgttt
    13861 ccctcgacaa ttcgcgctag acctagggcg ttcggctgcg gcgagcggta tcagctcact
    13921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    13981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    14041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    14101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    14161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    14221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    14281 gctgtgtgca cgaaccccc
//
