LOCUS       sequence-324188-        8556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DabAB1 inorganic carbon transport complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8556)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 8556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pFA-DAB1
FEATURES             Location/Qualifiers
     source          1..8556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      72..158
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(331..954)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     862..881
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(955..1059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1105..1122)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1239..1784)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1319..1338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1898..1992)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2013..2672)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2587..2606
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2673..2775)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2841..2859
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(2880..2898,2899..2904,2905..2921,2922..2927,
                     2928..2953)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    2880..2898
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2905..2923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2963..2974
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 agcgataaat taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatac
       61 tctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      121 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccgcta gcagttcgta
      181 gacggaaaca aacgcagaat ccaagcaaaa gggaataagg gcgacacgga aatgttgaat
      241 actcatactc ttcctttttc aatattattg aagcatttat cagggttatg tccactgggt
      301 tcgtgccttc atccggatat ccaggaactg ttaagaccca ctttcacatt taagttgttt
      361 ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg caccttggtg
      421 atcaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag taggtgtttc
      481 cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta aagtaaaatg
      541 ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt ggcataaaaa
      601 ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac ctaaatgtac
      661 ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt tattacgtaa
      721 aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc tatctaacat
      781 ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat acaatgtagg
      841 ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga ttccgacctc
      901 attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag acatactctt
      961 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1021 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgt ttccctcgac aattcgcgct
     1081 agacctaggg atatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     1141 gcggcgagcg gaaatggctt acgaacgggg cggagatttc ctggaagatg ccaggaagat
     1201 acttaacagg gaagtgagag ggccgcggca aagccgtttt tccataggct ccgcccccct
     1261 gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac aggactataa
     1321 agataccagg cgtttccccc tggcggctcc ctcgtgcgct ctcctgttcc tgcctttcgg
     1381 tttaccggtg tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag
     1441 ttccgggtag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     1501 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggaaagac atgcaaaagc
     1561 accactggca gcagccactg gtaattgatt tagaggagtt agtcttgaag tcatgcgccg
     1621 gttaaggcta aactgaaagg acaagttttg gtgactgcgc tcctccaagc cagttacctc
     1681 ggttcaaaga gttggtagct cagagaacct tcgaaaaacc gccctgcaag gcggtttttt
     1741 cgttttcaga gcaagagatt acgcgcagac caaaacgatc tcaagaagat catcttatta
     1801 atcagataaa atatttctag atttcagtgc aatttatctc ttcaaatgta gcacctgaag
     1861 tcagccccat acgatataag ttgttactag tgcttggatt ctcaccaata aaaaacgccc
     1921 ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt actggatcta
     1981 tcaacaggag tccaagcgag ctcgatatca aattacgccc cgccctgcca ctcatcgcag
     2041 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     2101 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     2161 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     2221 acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc
     2281 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     2341 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     2401 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     2461 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     2521 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     2581 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     2641 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     2701 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     2761 cttacgtgcc gatcaacgtc tcattttcgc cagatatcga cgtctaagaa accattatta
     2821 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cacctcgagt
     2881 ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag cacatcagca
     2941 ggacgcactg accgaattca ttaaagagga gaaaggtacc atgtccatga acctgcaatg
     3001 gttaattcca ttgttaccct tactgtccgc cctactggtg caattgtttg ccgctcgact
     3061 cggtaaacgt gttgcccacc tgagcgtggc gttgggaaca ctgaccgtca ttgttgccgc
     3121 atatcagctc gtagcgtata tcggtaccga tgcgagccca agctggcatt ctctgggcac
     3181 gatgtggggc agtctgtacg tcgatccgct gagcagcatc atgtcgctgg ttgtagcggg
     3241 catcagcctg attgttcatg tctattcaat ccgctacatg accgaagaac cgggctatcc
     3301 ccggttcttc ttgctgctgg atttgatgac cgcttccatt ttgcttatgg tcgccgccgg
     3361 tgacttggtc acattgctga tcgcctggca catgatcggc atcatgctct actttctttt
     3421 gggtcagaat acagaatcat ggccgagcca gcgctacgcc ttttggacgt ttatcacgta
     3481 ccggttgggg gacttgccat tggtgctcgc cgccgtgctg ctttatcaaa cctatggtgc
     3541 gattgacttc cccaccttat tttcccgtat cgcggccgat ccaaacgcca ccatcatggg
     3601 gctgcccacg gcgataacgg ctgctttttt ggtggcgctc tctgcctttg caaaatccgc
     3661 ccaattcccg ctacatacct ggctccccta taccatggag ggcccgacgc cggtttcagc
     3721 tttgatgcat gcggggattg tcaacgccgg tggttttatc atcaaccgct tcgcgcctgt
     3781 ttttgttcac agtgatggcg tactgcacat gctgtttgtc gtgggtctga ttaccgcgct
     3841 ggtgggatcg gtgctgatgt tgacgcaaaa cgacatcaaa aaatcactgg gttattccac
     3901 catgggtcaa atgggcttca tggtcatgga gtgtggtctt ggcgcttttt ctctggccgt
     3961 gttccatctc attgcgcatg gtctgttcaa aggcaccatg ttcctcggtt cgggcagcat
     4021 gattcatgaa gcacgcaagc acgacggcgt gccacataac cctctgcaca ccttcttggt
     4081 tgaacgaaag tctgcatcat taaaattgcc ttggttattt atcggcttgg ctacgctggt
     4141 cgtacctctg ttcattctgg ttattgcaca ctggttcgtg gcaccggatt ttttcgaaaa
     4201 acaaggtgcc atcgtgctgc tgttcttcgg ctggattacg ggggttcagg ttctttttgc
     4261 cacacatcat ctggatgcca ataacccaat tcgcatgatg atgatgattt tattgtcgtt
     4321 taccttgatt gtggtgggct acaccttcat tggtcatgct ttcgaaaact tcctttaccc
     4381 cgaagaagcc ttccgtaatg cgctgtacca tgcagcaggc atcgataaac tgacgttcga
     4441 cggacttgtt ttcttgctcg ctttgattgt ggtggcaggc tggtttagca gctatttggc
     4501 cagtcgtgag aaatcggttt tcggtgaccg gttcggagcg gttcgcctca ctctctactc
     4561 actgatctcg cgtgaatttt atgtagccga tctctacgac cggatggcgc actggctgct
     4621 tgatgcatcc aaacgcttca atgtctggat gaggtggtat tgatatgtcc tttttcttga
     4681 tgttactggc cgggttcttt ctgcccttgt tccccttgag catggtattc aatgccctgc
     4741 tcggccgaat caaaaacccc atcgcgcggg tactgctgat ttttatctgg cctcaaatcg
     4801 gcgttttctt gcttgcatgg gctggtttgc cgctggattc cggattcttc gttggctggg
     4861 cagtgcttac ggcagtcttc tatgcctggc gattgctgac ggtgcgtgtg ctctcccagt
     4921 gggccgggat gctcgccacg tccgcctggg cgctggcttg gattatcgcc gccgatcttc
     4981 cagccagtag cgaactgcac agtttcgccc tgtggatgtc cgttgcgccg gctttgctga
     5041 tcttcattgc cattgcactg caagaacgtt ttggcgctgc ttacagtggt ttagttgtgg
     5101 ggttggcgcg ggctttaccg cgcttatcca gcgtcattgt catcgtggtg ttggccgcaa
     5161 tggcactccc tattttcccc agtttttttg caatgttggc attgctggcg catagctcca
     5221 tcttattcgc tctgccgatt ttgcttacct ggttgctttg gagttgggca ggcacgcggt
     5281 tattgaatgg gtttgttttc gggccacgcg ccgagacaga tgccgccgat ctctcttcaa
     5341 ccacaacatg gggctttgtt ggcctgatag caacgattgt tgtgattaat ctgatttgga
     5401 caggagcttg atcccatgag caaactccct ttgggtaaac gcctgaaaat tcgttccatg
     5461 gtgcacatgg ctgccgagcc aatccccaat ttctggccta tgcggacgtt cattcaccat
     5521 aacccgctcc atggtctgga gcatctgccc ttcgaacagg cggttcgcca gggtgaaaaa
     5581 ctctttcacg cacggggatt tttgccgcgt gaggattacc agcgctatca caaggaaggc
     5641 cgagttgatc aaaacagcat aaagcgtgac atagccgatt ttatttcaaa acaagaaacg
     5701 ctcaacggtt tggatttggc atcgttactt agcgacttga tgtgttcggt taagaacaaa
     5761 gtaactagaa cgcgcgcgct cgccgatcat gatgatgtgt tccaagcctt gcacgggaaa
     5821 caactggaaa atgcagaggc gctcgatctt aaagcgctaa ctcaacgctt atgtgcgcag
     5881 tttgcaccag agcgccccct gtacgaagcc atcgatttgc tgttcggcac gcaaatgggc
     5941 accacactcg atgaattggt aatcaaaagc tgtctcgact ttttcgatga aggccaatca
     6001 accatccaaa tgcccggccg ccaccaagga ttgttcgcag cttggacggc cctggcaaaa
     6061 cgtaatttac gcctgttttt acgtggcatg catatcaaac agatcctcga tcaggacgat
     6121 acgccagagg gcatcatcgc ctacattctc gacgaactgg gcattgagga ggctcactgg
     6181 gatgggctga ttacccgcga actgactcgt ttgcatggct gggcaggttt tattcgctgg
     6241 cgctcctctt ccaagcacta ctattgggcc gagcagtacc cgggggatct catcgatttc
     6301 ctggccatcc ggctcgtttt aggcttggcc ttgatccgtg aacatagccg tcaaaagcgc
     6361 acaccgatga cagtcaaagt gctgcaagaa tatatcgaag ggcacaccgc cgaatgctat
     6421 ctgcgtcaag cctattacgg tggctgcata ttgcccgcgt ttgctcatga tgttgatgat
     6481 gcgctgtcgc ataaaaagcc tcaaaggatc aacaacattc ttccgggcta cctgcgccaa
     6541 caacgccaat tcgaggcaac acgacaagct gatgcgcttc gtgatttagc cagcaaagcg
     6601 gggcaaaccg atgcccttat ggcgctgaat gcgcctcaaa tcaagcaact catgacactt
     6661 atcgaggcgt ttgaaaacga agaaggcatg atctggcttc gtgcgatgga atcggtctat
     6721 cgacgggaaa tcatcaacca gattcaactg tatgcaccgc ataaaaaaga aaaacggccc
     6781 tttgcccagg cattgttctg tatcgatgtg cgctccgagc cgatacgccg taatctggaa
     6841 acggtaggcg agtatcaaac ctatggtatc gccgggtttt ttggtgttcc ggtaagctat
     6901 attggccttg gcaagggcag tgaagttaat ctttgcccgg tggtcattac ccctaaaaat
     6961 ctggtgcttg aagtgcccgt gggtgccaca agcattgaaa cagactttta ttcttccgcc
     7021 gaccatgtgc tacatgagat gaaaagctcg atcctttcac cctacttcac ggttgaagcg
     7081 gccggtttgc tgtttggttt cgacatgatc ggcaaaacca ttgccccgcg acgctacacc
     7141 caaatacgca atcatatcga accaaaagca caggcaactc gtttgctggt ggataaactc
     7201 acccgcgaac aagccgactc aatcgtccgt tcgctgcaac gcgccatgat tgtgcgcgcc
     7261 attcatcagg aatttggcat cgaacgcgaa gcagtcaccg atgccatgat ccgcgaactg
     7321 cgcgaagcgg ccatggataa ctatcacgaa cagaccgaat tcgcgcgccg tttcgccttg
     7381 agcccaacgg ccgaaactca gtttatcgca gggctgaaaa aggactataa aatcaatcgc
     7441 tcatttgttt ccatgcaaat ggaacgcttg gccaggattg gttttagcct cgatgaacag
     7501 gtgttttatg ttgacaaggc actcacatcc atcggattga ccgaaaactt ttcacgcttt
     7561 gttttattgg ccggtcacgg cagcacttcc gacaataatc cctacgaatc cgcgcttgac
     7621 tgcggtgcat gtggtggtag tcatgggctg gtttctgccc gggtgcttgc ccacatggcc
     7681 aataagcctg aagtacgtcg cagactggcc aagcaaggca tccagatacc tgaagatact
     7741 tggtttgtgt ccgtcatgca caacaccaca accgatcaat tgtcactgca agaccttgat
     7801 ttgcttccaa acagccatct tgtttacctc gaacgcttgc gtaacggctt acgtgcggcc
     7861 acccgtttgt cagcggcaga acgcttgcct gctctgcttg atcatccttc gcccaacatc
     7921 gacacattat cggcacaaaa acaaattgag cggaatgcca gcgactggac ccaagttcgg
     7981 ccagagtggg gcttggcgag aaatgcgagc gtcgtcgccg gcggccgaca tttgaccgag
     8041 ggtgcgaact taagcggtcg aacgtttttg cagtcttacg attatcgact cgatcccaaa
     8101 ggccgccacc ttgaaaacat tctcagcaac ccgctaatta tcggccagtg gatcaatctt
     8161 gagcattatt tctcagcggt agataacgaa cactttggca gtggcagcaa ggcctatcac
     8221 aacgtcgtag gtcgttttgg tgtggttacg ggtaatttaa gtgacttgcg aacagggtta
     8281 ccagcacagt cggtgcttaa agatggacgc ccataccacg agcccatccg tctcttggcg
     8341 attatcgaag cacccgcagc attcaccctc gaagtagcgg gtcgattgcc caaggtgatg
     8401 tccctgatta ccaacggctg gatcactgtt gttgtcgttg atccggaaac gggcgatcgt
     8461 cttttttatg atcgcggcga atggtacaat ctcaacaatg atccgcagta cacgccctcg
     8521 gtcaaaccct tgcttgaaga agaactcagc gcatga
//
