LOCUS       sequence-324189-       10132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses JIP4 in mammalian cells with a GFP tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, 
            Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, 
            Cookson MR
  TITLE     LRRK2 mediates tubulation and vesicle sorting from lysosomes.
  JOURNAL   Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 
            10.1126/sciadv.abb2454. Print 2020 Nov.
  PUBMED    33177079
REFERENCE   2  (bases 1 to 10132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-11-11"; volume: "6(46). pii: 6/46/eabb2454. doi"; pages:
            " 10.1126/sciadv.abb2454. Print 2020 Nov"
COMMENT     Sequence Label: GFP-JIP4
FEATURES             Location/Qualifiers
     source          1..10132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(905..907,908..910,911..1621)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(938..966)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1561..1581
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    1643..1667
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5667..5691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5723..5740)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5729..5953
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5999..6427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6086..6105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6296..6317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6436..6456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6441..6770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6621..6756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6683..6702
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6837..7631
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6891..6910)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7501..7520
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7805..7926
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7842..7861)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7896..7915
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7975..7991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7975..7991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7988..8010)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7999..8015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8023..8029,8030..8047,8048..8053))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8068..8089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8206..8223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8377..8965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8457..8476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9136..9927,9928..9996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9759..9778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9997..10101)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
      901 caccatggcc agcaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
      961 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgctac
     1021 atacggaaag cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     1081 aacacttgtc actactttct cttatggtgt tcaatgcttt tcccgttatc cggatcatat
     1141 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aacgcactat
     1201 atctttcaaa gatgacggga actacaagac gcgtgctgaa gtcaagtttg aaggtgatac
     1261 ccttgttaat cgtatcgagt taaaaggtat tgattttaaa gaagatggaa acattctcgg
     1321 acacaaactc gagtacaact ataactcaca caatgtatac atcacggcag acaaacaaaa
     1381 gaatggaatc aaagctaact tcaaaattcg ccacaacatt gaagatggat ccgttcaact
     1441 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     1501 ccattacctg tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat
     1561 ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa
     1621 aagcggttcc ggaccggatc aaacaagttt gtacaaaaaa gcaggcttaa ggagtttaaa
     1681 caccatggag ctggaggacg gtgtggtgta tcaggaggag cccggcggct ccggggccgt
     1741 gatgtcggag cgggtgtccg gcctggccgg ctccatctac cgcgagttcg agcggcttat
     1801 cgggcgctat gacgaggagg tggtcaaaga gctgatgccg ctggtggtgg ctgtgctgga
     1861 gaacctggac tcggtgttcg cgcaggacca ggagcaccag gtggagctgg agctgctgcg
     1921 ggacgacaac gagcagctca tcacccagta cgagcgggag aaggcgctgc gcaagcacgc
     1981 tgaggagaaa ttcattgaat ttgaagactc tcaagaacag gaaaaaaagg acttacagac
     2041 ccgagtggaa tctttagaat ctcaaacaag acaacttgag ctgaaagcga aaaactatgc
     2101 tgaccagatt agcagacttg aagaaagaga agcagaactg aagaaggaat ataatgcatt
     2161 acatcaaaga cacactgaga tgatccataa ttatatggaa catttagaaa gaacaaaact
     2221 tcatcagctc tcagggagtg atcaactaga atccacagct catagtagaa ttagaaaaga
     2281 acgccctata tcattaggaa ttttcccatt acctgctgga gatggattgc ttacacctga
     2341 tgctcagaaa ggaggagaga cccctggatc tgagcaatgg aaatttcagg aattaagtca
     2401 accacgttct cataccagcc tgaaggtcag caatagtcct gaacctcaga aggctgtaga
     2461 acaggaggat gagctttctg atgttagcca aggcggatct aaagctacca ctccagcatc
     2521 aacagctaat tcagatgtgg caacaattcc tactgatact cccttaaagg aagaaaacga
     2581 aggatttgtg aaggttacag atgcgccaaa taaatcagag ataagcaaac acattgaagt
     2641 acaggtagcc caggaaacta gaaatgtatc tactggctct gctgaaaatg aagaaaagtc
     2701 agaagttcaa gcaatcatcg aatctactcc tgagctggat atggacaaag atctcagtgg
     2761 atataaaggt tcaagcactc ccaccaaagg catagagaac aaagcttttg atcgcaatac
     2821 agaatctctc tttgaagaac tgtcttcagc tggctcaggc ctaataggag atgtggatga
     2881 aggagcagat ttactaggaa tgggtcggga agttgagaat cttatattag aaaatacaca
     2941 actgttggaa accaaaaatg ctttgaacat agtgaagaat gatttgatag caaaagtgga
     3001 tgaactgacc tgtgagaaag atgtgctgca aggggaattg gaggctgtga agcaagccaa
     3061 actgaaacta gaggaaaaga acagagaatt ggaggaagag cttaggaaag ctcgggcaga
     3121 agctgaagat gcaaggcaaa aagcaaaaga tgacgatgat agtgatattc ccacagccca
     3181 gaggaaacgg tttactagag tagaaatggc ccgtgttctc atggagcgaa accagtataa
     3241 agagagattg atggagcttc aggaagctgt tcgatggaca gagatgattc gggcatcacg
     3301 agaaaatcca gccatgcagg aaaaaaaaag gtcaagcatt tggcagtttt tcagccgact
     3361 tttcagctcc tcaagtaaca cgactaagaa gcctgaacca cctgttaatc tgaagtacaa
     3421 tgcacccacg tctcatgtta ctccgtccgt caagaaaaga agcagcacct tatctcagct
     3481 ccctggggat aagtccaaag cctttgattt ccttagtgaa gaaactgaag ctagtttagc
     3541 ctcacgcaga gaacaaaaga gagagcagta tcgtcaggta aaagcacatg ttcagaagga
     3601 agacggtaga gtgcaggctt ttggctggag tctgcctcag aagtacaaac aggtaaccaa
     3661 tggtcaaggt gaaaataaga tgaaaaattt acctgtgcct gtctatctca gacctctgga
     3721 tgaaaaagat acatcaatga agctgtggtg tgctgttgga gtcaatttat ctggtgggaa
     3781 gaccagagat ggtggttctg ttgttggagc aagtgtattt tacaaggatg ttgctggttt
     3841 ggatacagaa ggcagtaaac agcgaagtgc ctctcagagt agtttagata agttagatca
     3901 ggaacttaag gaacagcaga aggagttaaa aaatcaagaa gaattatcca gtctagtttg
     3961 gatctgtacc agcactcatt cggctacaaa agttcttatt attgatgctg ttcaacctgg
     4021 caacatccta gacagtttca ctgtttgcaa ctctcatgtt ctgtgcattg caagtgtgcc
     4081 aggtgcacga gaaacagact accctgcagg agaagatctt tcagaatctg gtcaggtaga
     4141 caaagcatct ttatgtggaa gtatgacaag caacagctca gcagagacag acagcctgtt
     4201 aggaggcatc acagtggttg gttgttctgc agaaggtgtg acgggagctg ccacttcccc
     4261 tagtacaaat ggtgcttctc cagtgatgga taaaccacca gaaatggaag cagaaaatag
     4321 tgaggttgat gaaaatgttc caacagcaga agaagcaact gaagctacag aagggaatgc
     4381 ggggtcagct gaagacacag tggacatctc ccaaactggc gtctacacag agcatgtctt
     4441 tacagatcct ttgggagttc agatcccaga agacctctcc ccagtgtatc agtcgagcaa
     4501 tgactcagat gcatataaag atcaaatatc agtactgcca aatgaacaag acttggtgag
     4561 agaagaagcc cagaaaatga gtagtctttt accaactatg tggcttggag ctcaaaatgg
     4621 ctgtttgtat gtccattcat ctgtagccca gtggaggaaa tgtctccatt ccattaaact
     4681 taaagattcg attctcagta ttgtacacgt gaagggaatc gtgttagtag ccctggctga
     4741 cggcaccctt gcaatctttc acagaggagt ggatgggcag tgggatttgt caaactatca
     4801 cctcttagac cttggacggc ctcatcattc catccgttgc atgactgtgg tacatgacaa
     4861 agtctggtgt ggctatagga acaaaatcta tgtggtgcag ccaaaggcca tgaaaataga
     4921 gaaatctttt gatgcacatc ccaggaagga gagccaagtg cgacagcttg cgtgggtggg
     4981 ggatggcgtg tgggtctcca ttcgcttgga ttctacgctc cgtctctatc atgcacacac
     5041 ttatcaacat ctacaggatg tggacattga gccttatgta agcaaaatgt taggtactgg
     5101 aaaactgggc ttctcttttg tgagaattac agctcttatg gtgtcttgta atcgtttgtg
     5161 ggtggggaca ggaaatggtg tcattatctc catcccattg acagaaacaa ataaaacctc
     5221 aggtgtacca ggaaatcgtc ctggaagtgt aatccgtgta tatggtgatg aaaacagtga
     5281 taaagtgact ccagggacat ttatacccta ttgttcaatg gcacatgcac agctttgctt
     5341 ccatgggcac cgggatgctg tgaaattctt tgtggcagtc ccaggtcaag tcatcagccc
     5401 acaaagtagc agtagtggca cggatctgac gggtgacaaa gcagggccat ctgcacagga
     5461 gcctggtagt cagacgccct tgaagtctat gcttgtcatc agtggaggag agggctacat
     5521 cgacttccga atgggtgatg aaggtggaga atcagaactt cttggagagg atcttccact
     5581 tgaaccttct gtcaccaaag cagaaaggag tcacttgata gtgtggcaag tgatgtatgg
     5641 caatgagtag ctcgagtgcg gccgcaaccc agctttcttg tacaaagtgg tgataattaa
     5701 ttaagataaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     5761 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     5821 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     5881 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     5941 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     6001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6421 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     6481 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     6541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     6601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     6661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     6721 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     6781 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     6841 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     6901 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     6961 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     7021 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7081 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7141 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7201 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7261 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7321 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     7381 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7441 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7501 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7561 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7621 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     7681 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7741 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7801 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7921 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     8041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     8101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     9001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     9061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
    10021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
    10081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
