LOCUS       sequence-32419-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4AD
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..62)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(215..231)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(216..232)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    422..491
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    536..569
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atattcccac tttgattaaa attggcggcg gtacccaatt cgacctttct cttctttttt
       61 ggaggctcgg gaattaattc cgctttatcc atgttttgcg gccgcggatc cacgtaaggg
      121 ttaatgtttt caaaaaaaaa ttcgtccgca cacaaccttt cctctcaaca agcaaacgtg
      181 cactgaattt aagtgtatac ttcggtaagc ttatcgatac cgtcgacctc gacatgcccg
      241 ccgtgaccgt cgagaacccg ctgacgctgc cccgcgtatc cgcacccgcc gacgccgtcg
      301 cacgtcccgt gctcaccgtg accaccgcgc ccagcggttt cgagggcgag ggcttcccgg
      361 tgcgccgcgc gttcgccggg atcaactacc gccacctcga cccgttcatc atgatggacc
      421 agatgggtga ggtggagtac gcgcccgggg agcccaaggg cacgccctgg cacccgcacc
      481 gcggcttcga gaccgtgacc tacatcgtcg agggggggcc cggtactggc gcgccgaagt
      541 tcctattctc tagaaagtat aggaacttcg gtaccgaatt cggctgctgc tctaaacgac
      601 gcatttcgta ctccaaagta cgaatttttt ccctcaagct cttattttca ttaaacaatg
      661 aacaggacct aacgcacagt cacgttattg tttacataaa tgattttttt tactattcaa
      721 acttactctg tttgtgtact cccactggta tagccttctt ttatcttttc tggttcaggc
      781 tctatcactt tactaggtac ggcatctgcg ttgagtcgcc tccttttaaa tgtctgacct
      841 tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg tatcacaaat ttgggagttt
      901 tcaccaaggc tgcacccaag g
//
