LOCUS       sequence-324197-       14585 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Second CCM operon with inactive CbbQ (K46A, E107Q) rubisco activase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14585)
  AUTHORS   Flamholz AI, Dugan E, Blikstad C, Gleizer S, Ben-Nissan R, Amram S, 
            Antonovsky N, Ravishankar S, Noor E, Bar-Even A, Milo R, Savage D
  TITLE     Functional reconstitution of a bacterial CO2 concentrating mechanism
            in E. coli.
  JOURNAL   Elife. 2020 Oct 21;9. pii: 59882. doi: 10.7554/eLife.59882.
  PUBMED    33084575
REFERENCE   2  (bases 1 to 14585)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-10-21"; volume: "9. pii: 59882. doi"; pages: " 
            10.7554/eLife.59882"
COMMENT     Sequence Label: pCCM' CbbQ-
FEATURES             Location/Qualifiers
     source          1..14585
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        443..547
                     /gene="bla"
                     /label=AmpR promoter
     CDS             548..1171
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVAQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(621..640)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(1344..1430)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             13100..13111
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(13121..13146,13147..13152,13153..13169,
                     13170..13175,13176..13194))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    13151..13169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    13176..13194
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(13215..13233)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13299..13401
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             13402..14061
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(13468..13487)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      14082..14176
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(14264..14585,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gccctaggtc
      421 tagcgcgaat tgtcgaggga aacgcggaac ccctatttgt ttatttttct aaatacattc
      481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
      541 gaagagtatg tctagattag ataaaagtaa agtgattaac agcgcattag agctgcttaa
      601 tgaggtcgga atcgaaggtt taacaacccg taaactcgcc cagaagctag gtgtagcgca
      661 gcctacattg tattggcatg taaaaaataa gcgggctttg ctcgacgcct tagccattga
      721 gatgttagat aggcaccata ctcacttttg ccctttagaa ggggaaagct ggcaagattt
      781 tttacgtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      841 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
      901 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
      961 gcattttact ttaggttgcg tattggaaga tcaagagcat caagtcgcta aagaagaaag
     1021 ggaaacacct actactgata gtatgccgcc attattacga caagctatcg aattatttga
     1081 tcaccaaggt gcagagccag ccttcttatt cggccttgaa ttgatcatat gcggattaga
     1141 aaaacaactt aaatgtgaaa gtgggtctta acagttcctg gatatccgga tgaaggcacg
     1201 aacccagtgg acataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     1261 tattcaacat ttccgtgtcg cccttattcc cttttgcttg gattctgcgt ttgtttccgt
     1321 ctacgaactg ctagcggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
     1381 cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctctag
     1441 agtattacaa tagctaagaa tttcgtcatc gctgaataca gttacatttt agaacccttc
     1501 agcgcgacgc gcacgcgcgt tgatgcttgc gattgtggcc tcagccgccc gcatggcttc
     1561 ttcggtttcc gcttcgctgc ccatcatggt caggcgacca aagttaccaa aagcacgtac
     1621 atcgatcaga gtcacatgag ctgctttctc ggcctcgttg gcagcctgaa caatataacc
     1681 tgccggttct gtttcaagga tgaacatgct tttgcccggc aacagcatag agcctttacg
     1741 caactggcga ttgatcaagg tggcatgatc gggggtaatc gctcgaatga tctctttcca
     1801 tgcgatgcga caaggcagac gatcttcttg cgcgtgattg atttctctta aaacggcctc
     1861 accggatgcg agaacgtcgc tttggtttcg gtaatgaatt tccatcgagc caaaagcacg
     1921 ctcgactacc tgttcgccga gacgaacgtt cgtggccttt agcgcaatat cactgaggcg
     1981 atggacggcc atgcccggcg cgacttcaat ccacaagcag gcatcacccg gaacgggaag
     2041 aaagccttgc gatgatgtcg caagatagga tgcaagctgc ggttgaagcg aatcgataaa
     2101 cgaatagacg cgcaaatcaa tgttgttcat gcgcatcttc cctgaagatg cacactgatt
     2161 tcattgattt tcacttgtat cttgatttgg gcggatacag tttggtctgg agttttaatc
     2221 atcatcggac agttccgggt cccatccatt ttcccgggcg cgttttagtg catcttcctg
     2281 aattcttagg tacttttctt taagctcggg tggcaagcgg ctcgggatgt gtgcatacgg
     2341 ttcccaggca gcaaacacct tttcatacag catttgaatc tgtgtgtggg tccagccggt
     2401 acccgtttcc gtttctggaa taaagccaaa cagccaacca tctcggataa tttttccgat
     2461 gtgcgtcatg ccgccttgtg cgtcaagatc aagaccgaca tagctggtgt gtttcatgtt
     2521 gtgcgtatta cctgttggta cggaaggctt aaaagaacgt tttgacgacg gtgtcgagtg
     2581 catcgcgcat gtcgacatca tctgtgatcg ggttgacaag cgtcatggtg caggcagcgc
     2641 gggcatcaac gcctttggca atcaatttgc ctgcatagac taacagacgc gtggagaggc
     2701 cttcatccag gccatgaccc ttgaggttac gtgctttttg tgcgatctga accagttttt
     2761 cagcagtgtc tttatcaacc ccggcctcgt ggctcacaat ttcggcttca atgtctggtt
     2821 tagggtagtc aaaatcaagc gcaccaaaac gttgcttggt ggattgcttg aggtctttca
     2881 tcaggctttg atagcccgga ttgtaagaaa tcacgatttg gaaatcggga tgcgcttcaa
     2941 ccaattcgcc ctttttctca agtggcagta ctcgacggtg atcggtcaat ggatggatga
     3001 cgacgatggt atcctgacgg gcctcaacaa tctgatcgag gtagcagatg gcaccaatgc
     3061 gcgcagcgac cgtaagcggg ccatcttgcc aacgggtgcc atctttatcg agcaaatagc
     3121 ggccgacaag atcggatgcc gtcatgtctt cattacacgc gatggtgatt aaggggcggt
     3181 tgagcttcca agccatatgt tcgacaaaac gtgaagcacc acagccggtc gggcctttga
     3241 gcatcatggg aatgcgtgct tcgtaaccgg cgcgatataa ctcgatttcg tcttgtacag
     3301 ctcgataata gggctctttg cgtacgcgat attgatctgc attttgtgtc atttaaattg
     3361 ccttgttctg gtcgatgtag ccgtctttct gatttaaatc gtatttattt agcgtctgtt
     3421 ttgaacttga ccggacgact gacctgaatg tcagaaacaa atactactcc gctgtgtttg
     3481 ctgaaaaagg gggcgaagcc ttccagcaag ggagaaacga gctcttccgg aacggcggtg
     3541 atgatcatgt tcagcacttc atcttcgtta aacatgagat ggccttcatg aaagccttga
     3601 gcccctttgc ctgaaaggtt gccgacaatg gtgtagccct tgacgcctgc tcggtcgagt
     3661 aaatccgtgg cgaatgattg atgttcacct tcgaggatga tttccagttt tttcaattgg
     3721 gtaaggttga ggtttttcat gcgctgagtt cttcttcaag caagggtttg accgagggcg
     3781 tgtactgcgg atcattgttg agattgtacc attcgccgcg atcataaaaa agacgatcgc
     3841 ccgtttccgg atcaacgaca acaacagtga tccagccgtt ggtaatcagg gacatcacct
     3901 tgggcaatcg acccgctact tcgagggtga atgctgcggg tgcttcgata atcgccaaga
     3961 gacggatggg ctcgtggtat gggcgtccat ctttaagcac cgactgtgct ggtaaccctg
     4021 ttcgcaagtc acttaaatta cccgtaacca caccaaaacg acctacgacg ttgtgatagg
     4081 ccttgctgcc actgccaaag tgttcgttat ctaccgctga gaaataatgc tcaagattga
     4141 tccactggcc gataattagc gggttgctga gaatgttttc aaggtggcgg cctttgggat
     4201 cgagtcgata atcgtaagac tgcaaaaacg ttcgaccgct taagttcgca ccctcggtca
     4261 aatgtcggcc gccggcgacg acgctcgcat ttctcgccaa gccccactct ggccgaactt
     4321 gggtccagtc gctggcattc cgctcaattt gtttttgtgc cgataatgtg tcgatgttgg
     4381 gcgaaggatg atcaagcaga gcaggcaagc gttctgccgc tgacaaacgg gtggccgcac
     4441 gtaagccgtt acgcaagcgt tcgaggtaaa caagatggct gtttggaagc aaatcaaggt
     4501 cttgcagtga caattgatcg gttgtggtgt tgtgcatgac ggacacaaac caagtatctt
     4561 caggtatctg gatgccttgc ttggccagtc tgcgacgtac ttcaggctta ttggccatgt
     4621 gggcaagcac ccgggcagaa accagcccat gactaccacc acatgcaccg cagtcaagcg
     4681 cggattcgta gggattattg tcggaagtgc tgccgtgacc ggccaataaa acaaagcgtg
     4741 aaaagttttc ggtcaatccg atggatgtga gtgccttgtc aacataaaac acctgttcat
     4801 cgaggctaaa accaatcctg gccaagcgtt ccatttgcat ggaaacaaat gagcgattga
     4861 ttttatagtc ctttttcagc cctgcgataa actgagtttc ggccgttggg ctcaaggcga
     4921 aacggcgcgc gaattcggtc tgttcgtgat agttatccat ggccgcttcg cgcagttcgc
     4981 ggatcatggc atcggtgact gcttcgcgtt cgatgccaaa ttcctgatga atggcgcgca
     5041 caatcatggc gcgttgcagc gaacggacga ttgagtcggc ttgttcgcgg gtgagtttat
     5101 ccaccagcaa acgagttgcc tgtgcttttg gttcgatatg attgcgtatt tgggtgtagc
     5161 gtcgcggggc aatggttttg ccgatcatgt cgaaaccaaa cagcaaaccg gccgcttcaa
     5221 ccgtgaagta gggtgaaagg atcgagcttt tcatctcatg tagcacatgg tcggcggaag
     5281 aataaaagtc tgtttcaatg cttgtggcac ccacgggcac ttcaagcacc agatttttag
     5341 gggtaatgac caccgggcaa agattaactt cactgccctt gccaaggcca atatagctta
     5401 ccggaacacc aaaaaacccg gcgataccat aggtttgata ctcgcctacc gtttccagat
     5461 tacggcgtat cggctcggag cgcacatcga tacagaacaa tgcctgggca aagggccgtt
     5521 tttctttttt atgcggtgca tacagttgaa tctggttgat gatttcccgt cgatagaccg
     5581 attccatcgc acgaagccag atcatgcctt cttcgttttc aaacgcctcg ataagtgtca
     5641 tgagttgctt gatttgaggc gcattcagcg ccataagggc atcggtttgc cccgctttgc
     5701 tggctaaatc acgaagcgca tcagcttgtc gtgttgcctc gaattggcgt tgttggcgca
     5761 ggtagcccgg aagaatgttg ttgatccttt gaggcttttt atgcgacagc gcatcatcaa
     5821 catcatgagc aaacgcgggc aatatgcagc caccgtaata ggcttgacgc agatagcatt
     5881 cggcggtgtg cccttcgata tattcttgca gcactttgac tgtcatcggt gtgcgctttt
     5941 gacggctatg ttcacggatc aaggccaagc ctaaaacgag ccggatggcc aggaaatcga
     6001 tgagatcccc cgggtactgc tcggcccaat agtagtgctt ggaagaggag cgccagcgaa
     6061 taaaacctgc ccagccatgc aaacgagtca gttcgcgggt aatcagccca tcccagtgag
     6121 cctcctcaat gcccagttcg tcgagaatgt aggcgatgat gccctctggc gtatcgtcct
     6181 gatcgaggat ctgtttgata tgcatgccac gtaaaaacag gcgtaaatta cgttttgcca
     6241 gggccgtcca agctgcgaac aatccttggt ggcggccggg catttggatg gttgattggc
     6301 cttcatcgaa aaagtcgaga cagcttttga ttaccaattc atcgagtgtg gtgcccattt
     6361 gcgtgccgaa cagcaaatcg atggcttcgt acagggggcg ctctggtgca aactgcgcac
     6421 ataagcgttg agttagcgct ttaagatcga gcgcctctgc attttccagt tgtttcccgt
     6481 gcaaggcttg gaacacatca tcatgatcgg cgagcgcgcg cgttctagtt actttgttct
     6541 taaccgaaca catcaagtcg ctaagtaacg atgccaaatc caaaccgttg agcgtttctt
     6601 gttttgaaat aaaatcggct atgtcacgct ttatgctgtt ttgatcaact cggccttcct
     6661 tgtgatagcg ctggtaatcc tcacgcggca aaaatccccg tgcgtgaaag agtttttcac
     6721 cctggcgaac cgcctgttcg aagggcagat gctccagacc atggagcggg ttatggtgaa
     6781 tgaacgtccg cataggccag aaattgggga ttggctcggc agccatgtgc accatggaac
     6841 gaattttcag gcgtttaccc aaagggagtt tgctcatggg atcaagctcc tgtccaaatc
     6901 agattaatca caacaatcgt tgctatcagg ccaacaaagc cccatgttgt ggttgaagag
     6961 agatcggcgg catctgtctc ggcgcgtggc ccgaaaacaa acccattcaa taaccgcgtg
     7021 cctgcccaac tccaaagcaa ccaggtaagc aaaatcggca gagcgaataa gatggagcta
     7081 tgcgccagca atgccaacat tgcaaaaaaa ctggggaaaa tagggagtgc cattgcggcc
     7141 aacaccacga tgacaatgac gctggataag cgcggtaaag cccgcgccaa ccccacaact
     7201 aaaccactgt aagcagcgcc aaaacgttct tgcagtgcaa tggcaatgaa gatcagcaaa
     7261 gccggcgcaa cggacatcca cagggcgaaa ctgtgcagtt cgctactggc tggaagatcg
     7321 gcggcgataa tccaagccag cgcccaggcg gacgtggcga gcatcccggc ccactgggag
     7381 agcacacgca ccgtcagcaa tcgccaggca tagaagactg ccgtaagcac tgcccagcca
     7441 acgaagaatc cggaatccag cggcaaacca gcccatgcaa gcaagaaaac gccgatttga
     7501 ggccagataa aaatcagcag tacccgcgcg atggggtttt tgattcggcc gagcagggca
     7561 ttgaatacca tgctcaaggg gaacaagggc agaaagaacc cggccagtaa catcaagaaa
     7621 aaggacatat caataccacc tcatccagac attgaagcgt ttggatgcat caagcagcca
     7681 gtgcgccatc cggtcgtaga gatcggctac ataaaattca cgcgagatca gtgagtagag
     7741 agtgaggcga accgctccga accggtcacc gaaaaccgat ttctcacgac tggccaaata
     7801 gctgctaaac cagcctgcca ccacaatcaa agcgagcaag aaaacaagtc cgtcgaacgt
     7861 cagtttatcg atgcctgctg catggtacag cgcattacgg aaggcttctt cggggtaaag
     7921 gaagttttcg aaagcatgac caatgaaggt gtagcccacc acaatcaagg taaacgacaa
     7981 taaaatcatc atcatcatgc gaattgggtt attggcatcc agatgatgtg tggcaaaaag
     8041 aacctgaacc cccgtaatcc agccgaagaa cagcagcacg atggcacctt gtttttcgaa
     8101 aaaatccggt gccacgaacc agtgtgcaat aaccagaatg aacagaggta cgaccagcgt
     8161 agccaagccg ataaataacc aaggcaattt taatgatgca gactttcgtt caaccaagaa
     8221 ggtgtgcaga gggttatgtg gcacgccgtc gtgcttgcgt gcttcatgaa tcatgctgcc
     8281 cgaaccgagg aacatggtgc ctttgaacag accatgcgca atgagatgga acacggccag
     8341 agaaaaagcg ccaagaccac actccatgac catgaagccc atttgaccca tggtggaata
     8401 acccagtgat tttttgatgt cgttttgcgt caacatcagc accgatccca ccagcgcggt
     8461 aatcagaccc acgacaaaca gcatgtgcag tacgccatca ctgtgaacaa aaacaggcgc
     8521 gaagcggttg atgataaaac caccggcgtt gacaatcccc gcatgcatca aagctgaaac
     8581 cggcgtcggg ccctccatgg tataggggag ccaggtatgt agcgggaatt gggcggattt
     8641 tgcaaaggca gagagcgcca ccaaaaaagc agccgttatc gccgtgggca gccccatgat
     8701 ggtggcgttt ggatcggccg cgatacggga aaataaggtg gggaagtcaa tcgcaccata
     8761 ggtttgataa agcagcacgg cggcgagcac caatggcaag tcccccaacc ggtacgtgat
     8821 aaacgtccaa aaggcgtagc gctggctcgg ccatgattct gtattctgac ccaaaagaaa
     8881 gtagagcatg atgccgatca tgtgccaggc gatcagcaat gtgaccaagt caccggcggc
     8941 gaccataagc aaaatggaag cggtcatcaa atccagcagc aagaagaacc ggggatagcc
     9001 cggttcttcg gtcatgtagc ggattgaata gacatgaaca atcaggctga tgcccgctac
     9061 aaccagcgac atgatgctgc tcagcggatc gacgtacaga ctgccccaca tcgtgcccag
     9121 agaatgccag cttgggctcg catcggtacc gatatacgct acgagctgat atgcggcaac
     9181 aatgacggtc agtgttccca acgccacgct caggtgggca acacgtttac cgagtcgagc
     9241 ggcaaacaat tgcaccagta gggcggacag taagggtaac aatggaatta accattgcag
     9301 gttcatcatg cgctttctcc atcacagacc ggccttggcc gggtagttat cttgcgtata
     9361 cgcaaattaa aattaatcgg gtttcaggcg cattgtcctc gtatcccgag cagcaatttg
     9421 aacagcttca tgagatttgt ttcttgtctg aagctcggac ggaaagttct atcgcgtcat
     9481 cgacaaataa agtgccggta gtttctcagg cagtcgagct acatggtcga ggatcatgta
     9541 gtgtttcggg ccaaaaattc gttcgacata atcatcggct cttggatcga gggtgacgca
     9601 gaacgtattg atgccccgtt tccctaattc ctcaacaacg cgcttggtgt cttggcgtag
     9661 atattggggg tcgcgcacat cattatcgga aggctcgcca tcggtaagta acagaataag
     9721 tttcttctgg ctgggccgtg aatccaacaa atggcccgcg tgtcgcaagg cagtgcccat
     9781 gcgtgtggag agttgccccg tcattccggc cagacgagcc ttggcgaggt catcgaaggc
     9841 gtcatcaaag tctttgaagc ggaaatactc aacatcgctg cgcccattgg aatcaaaacc
     9901 atgaatagcg tagggatcac caatgcgatt caatgcatca ccgagtaatg ctgctgcctc
     9961 tctagccata tcaagtacgg ttttttcttc ttcaccggtt tgcacgagat cattggttga
    10021 ttctgagaga tcaatgagca aaatcacgga tagatcacgc acatggatac gtcgacgaat
    10081 gccaatgcgc gggtccggtt gacgattcat tcgaatatcg atcattgcgc caatggctgc
    10141 attgagatcg acttcatcac catcctcgat ttttttctgt cgcacgacac cttgtggctg
    10201 taagccttcg atcaggtact tcaaacgccg taccattggg gcgtgctcga acaaaatatt
    10261 atcaaccacc gcaggatcgc ccaatttggg tttcttctcc atcagggtcg tccacaatgg
    10321 tcgctcaagc tgtgattggt aatcccattc gggataatat cgaggcgaag cagtcggtgg
    10381 cgtgccataa agctcattga acgtcgtgcc atcgtcatca aaaagttcgg tggcaagcac
    10441 ccatatttca tccgcgcttt ccgccgtggc gtactccaca tccaaggcgt gcagcatctg
    10501 catcacgcta acgtacttgc gcttttcctg aatgctggcc gcggtatgtg cattgctctt
    10561 gcctgtttcc ggtgtttgcc acaaataggt attgtcatca cggtacgggc tacttgggat
    10621 atcggtacgg ctttgaaata ccaggttttg ctgctggatc ttttcggcta gagcgatgcc
    10681 cagttctcgc gccatctcct gatcttccag acgatcaagt gctgctttaa acgcctcacg
    10741 cgaccattcg acaagcgggt gggtatccga tgaattctca tcgcatagtg ccagcgctat
    10801 ccggtcgaaa agtgcgccaa gagttggatt ctctcctgtt cgtgcgtcag cggggagggc
    10861 agcaaccgtc agttggttga gattcctcca caggggaacc aatccaggga aatcggccat
    10921 tgcgagtttt tcgacacgcg cgtcttcaaa taaaccaatg gtcgcgcggt ccagtgggga
    10981 tgcgttcttc gcatcggagt cgagacgcga ataaatttga tgtgctgcgg catgagccgc
    11041 tgccgcacga tagacagtca gaccatcaac tgtttgttcg ccttcaacgg cgtaatcatc
    11101 atacgcgtcg ggtaaattga taaatagttc atcaatgaac ggacggtatc ccgcacggct
    11161 ttcgaaatca cccgaagtgg ggcgtagaaa aaaatcacgc ccccacagtg cgcgtaaata
    11221 catcaccaag cggcgttgta catcaacaaa caatacaccc cttcgctcgc gctgtaacac
    11281 agcacgggat tcaggcgttg caagactaaa gtaggcctct tgggcggcaa aatcggttcg
    11341 gtgcgcttga atcccccaag tgatccaacg ccgcaatccc ccaagcgtca gcacaccaag
    11401 cagttgctct atttgaccaa gaagtggacg taaggctcgg ggggctttgg ccgctatgta
    11461 atccagtaaa gaaagatagg caacgaacag ttcagcctca ccgagccgac tgacagcaac
    11521 aggtgccgat ttgaaaacca ttgctaaaac ttcagcactg gccttggaga acattttaat
    11581 aacggaagac agcagttcga aaactgctgt ttcaccaatt gaccttgtaa caggacctgc
    11641 cgattcaata aacgagataa ctaactcatc accgcgtccc aaagccttaa gattggaaac
    11701 gctttctaga aaagccttga aacccatcgg gctgaacaat cgcgaaagct caatccaatg
    11761 ggtctcaagg acttcagcga tcggttcgcc caagtcttca aaaagagcct gatattcttc
    11821 agtcgctggg ttcatagttt gacgttagtt tcgcgatgtt cggacaaaat caatctggcc
    11881 atacacggcg aggatgcgtc gatgtgtcgt gtgccggaac ctgaatatca tcagccgatg
    11941 gcttgctgga ttttgataca gcaggttttg ttgtcgtcgc acgcgctaca ggctttttcg
    12001 cagccgtccg tgtggcaggt ttggcccctg tcgatgcagg cttggtggtt tcgggtgcag
    12061 ccgagcgcgc ggctggtttg gctggcgttg acgcaggcgc tatcgtgtcc ttgttctcgg
    12121 tcttgatgct tgcactcagt ttatcttcta aattagtcat gacgggataa cctcgttaag
    12181 gatttgattg atttcatcag ccgctgagcg gcctcttgct ccgagctgga aaacgctaac
    12241 gccttccact acggcatttc ggtacacagc gcgtcgtcgc acagaggtag tgagtacggg
    12301 aacccctagt cgctcaatgg ttgtttgcat tgctcgtgtc attgcactgc gcggctctgt
    12361 ttgattgagt accaacgcag cttttagctt ggggttggtt ttgcgtgcgc tttcaatcat
    12421 ctcgattgtt tgagtgctcg cccaaagatc taaaggggag ggcagtacag gaattaatat
    12481 aaaatcgcac tcaatcatca attgacaggt aattgccatg tctagggaag gcgggcaatc
    12541 cacgacgaca aagtcatggt ttggttgcgc caatttaaga ttttgaacgg gatcggtgtg
    12601 atctgagtat aagacgggga cttgagcgtc cgcctctttg cttccccagt caacccaatg
    12661 cttgagtgcg ccttgggggt cggcatcgac cagaccaaca cgaccacgct gcgtcaagcc
    12721 cgcactgatg tttgtgctga tggtcgtctt gccacagcca cctttgaggt tggcgacagc
    12781 caatgtaaag gctttgttgc tggccatggc gttagctata cttggcggcc aagtcattga
    12841 cttgggtagc aaattcaacc tcagactctt gcaatggacc gtcgtccgca gcagatatgg
    12901 tgacggtcac gaattctttg gcaaaatgca agttgggata gttgccaatg gcctcagata
    12961 aatcagctaa atcgtccaaa aatgaacgcg tttgagaata acttccaaaa tcgaagcggc
    13021 gagttaattg aggtggtaat ttacggcatt cccaaccatc aacctcaatc tgatttagct
    13081 tttctatcgc catggtacct ttctcctctt taatgaattc ggtcagtgcg tcctgctgat
    13141 gtgctcagta tctctatcac tgatagggat gtcaatctct atcactgata gggactcgag
    13201 gtgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
    13261 ggtttcttag acgtcgatat ctggcgaaaa tgagacgttg atcggcacgt aagaggttcc
    13321 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
    13381 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
    13441 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
    13501 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
    13561 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
    13621 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
    13681 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
    13741 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
    13801 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
    13861 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
    13921 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
    13981 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
    14041 gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt tgatagatcc
    14101 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
    14161 ttttattggt gagaatccaa gcactagtca tgaccaaaat cccttaacgt gagttttcgt
    14221 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    14281 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    14341 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    14401 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    14461 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    14521 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    14581 gaacg
//
