LOCUS       sequence-32420-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     174..192
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    377..410
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggcaacagcg ctgactttga gtggaatgtc attttgagtg agaggtaatc gaaagaacct
       61 ggtacatcaa atacccttgg atcgaagtaa atttaaaact gatcagataa gttcaatgat
      121 atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac tttccgcaaa aatgggtttt
      181 attaacttac atacatacta gaattcgatc tctcgtggga tcattgtttt tctcttgatt
      241 cccactttgt ggttctaagt actgtggttt ccaaatgtgt cagtttcata gcctgaagaa
      301 cgagatcagc agcctctgtt ccacatacac ttcattctca gtattgtttt gccaagttct
      361 aattccatca gaagctataa cttcgtataa tgtatgctat acgaagttat ctagcctagg
      421 catgcttaat taagctagtg gatctaaacg agtttttaag caaactcact ccctgacaat
      481 aaaaacgcaa ttatctttaa ataaatatat tctatttatt aaccaagtag ctaatatagc
      541 taaaatcaat ttgttgctaa cttaaaacta acataattat gtattagtta tttacattct
      601 tttggttggt tacaaccaaa taaataatat cacttttaaa aacttaagcc aggaactgaa
      661 taattttcta tttggcttta gtcgacggta tcgatctaga ggcgcgcctt acgatacagt
      721 caactgtctt tgacctttgt tactactctc ttccgatgat gatgtcgcac ttattctatg
      781 ctgtctcaat gttagaggca tatcagtctc cactgaagcc aatctatctg tgacggcatc
      841 tttattcaca ttatcttgta caaataatcc tgt
//
