LOCUS       sequence-32421-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(137..170)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(194..227)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(457..658,659..689)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata
       61 tatcaaaaat aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc
      121 actggaacta gaattcataa cttcgtatag catacattat acgaagttat ggatctggcc
      181 gcgctcttcc gctgaagttc ctatactttc tagagaatag gaacttcctc gacggtagcg
      241 gtccggttgt tttcgtgctc atcgcgagta cgtatttatt tttaaaaaat aataaacgcg
      301 gcaaacacaa tcacacaaat gtgcaggcac agtgggacaa agtgcgctaa atttggatat
      361 gtaataaatc caaaaccaaa aagaaaacaa aaaacggtaa aatattttac tgttgttaaa
      421 attcgatcat tcattattcg ctgcatgaat tagctttgcg tactcgcaaa ttattaaaaa
      481 taaaacttta aaaataattt cgtctaatta atattatgag ttaattcaaa ccccacggac
      541 atgctaaggg ttaatcaaca atcatatcgc tgtctcactc agactcaata cgacactcag
      601 aatactattc ctttcactcg cacttattgc aagcatacgt taagtggatg tctcttgccg
      661 acgggaccac cttatgttat ttcatcatgg tctggccatt ctcatcgtga gcttccgggt
      721 gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga catatatccg
      781 aaataactgc ttgttttttt ttttaccatt attaccatcg tg
//
