LOCUS       sequence-324210-       11321 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR_Cas9, lambda red recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11321)
  AUTHORS   McConville TH, Annavajhala MK, Giddins MJ, Macesic N, Herrera CM, 
            Rozenberg FD, Bhushan GL, Ahn D, Mancia F, Trent MS, Uhlemann AC
  TITLE     CrrB Positively Regulates High-Level Polymyxin Resistance and 
            Virulence in Klebsiella pneumoniae.
  JOURNAL   Cell Rep. 2020 Oct 27;33(4):108313. doi: 
            10.1016/j.celrep.2020.108313.
  PUBMED    33113377
REFERENCE   2  (bases 1 to 11321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-10-27"; volume: "33(4):108313. doi"; pages: " 
            10.1016/j.celrep.2020.108313"
COMMENT     Sequence Label: pUC19_CRISPR_DpmrA
FEATURES             Location/Qualifiers
     source          1..11321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        171..455
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             499..4605
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             499..4602
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     terminator      complement(4644..4888)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     CDS             complement(4889..5569)
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             complement(5566..6351)
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             complement(6357..6773)
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     RBS             6782..6790
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(6817..7101)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     CDS             7128..8006
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRH
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(8133..8149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8142..8164)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(8294..8299,8300..8315,8316..8322)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_RNA        8348..8423
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(8498..8517)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8617..8639
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     regulatory      8880..8889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8886..9260
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(9375..9393)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      10446..11034
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10935..10954
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11188..11205
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cttgcatgcc tgcaggtttt catactcccg ccattcagag aagaaaccaa
      181 ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac
      241 caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac
      301 aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca
      361 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
      421 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg aacctgcagg
      481 tcgacgaggt tataaaaaat ggataagaaa tactcaatag gcttagatat cggcacaaat
      541 agcgtcggat gggcggtgat cactgatgaa tataaggttc cgtctaaaaa gttcaaggtt
      601 ctgggaaata cagaccgcca cagtatcaaa aaaaatctta taggggctct tttatttgac
      661 agtggagaga cagcggaagc gactcgtctc aaacggacag ctcgtagaag gtatacacgt
      721 cggaagaatc gtatttgtta tctacaggag attttttcaa atgagatggc gaaagtagat
      781 gatagtttct ttcatcgact tgaagagtct tttttggtgg aagaagacaa gaagcatgaa
      841 cgtcatccta tttttggaaa tatagtagat gaagttgctt atcatgagaa atatccaact
      901 atctatcatc tgcgaaaaaa attggtagat tctactgata aagcggattt gcgcttaatc
      961 tatttggcct tagcgcatat gattaagttt cgtggtcatt ttttgattga gggagattta
     1021 aatcctgata atagtgatgt ggacaaacta tttatccagt tggtacaaac ctacaatcaa
     1081 ttatttgaag aaaaccctat taacgcaagt ggagtagatg ctaaagcgat tctttctgca
     1141 cgattgagta aatcaagacg attagaaaat ctcattgctc agctccccgg tgagaagaaa
     1201 aatggcttat ttgggaatct cattgctttg tcattgggtt tgacccctaa ttttaaatca
     1261 aattttgatt tggcagaaga tgctaaatta cagctttcaa aagatactta cgatgatgat
     1321 ttagataatt tattggcgca aattggagat caatatgctg atttgttttt ggcagctaag
     1381 aatttatcag atgctatttt actttcagat atcctaagag taaatactga aataactaag
     1441 gctcccctat cagcttcaat gattaaacgc tacgatgaac atcatcaaga cttgactctt
     1501 ttaaaagctt tagttcgaca acaacttcca gaaaagtata aagaaatctt ttttgatcaa
     1561 tcaaaaaacg gatatgcagg ttatattgat gggggagcta gccaagaaga attttataaa
     1621 tttatcaaac caattttaga aaaaatggat ggtactgagg aattattggt gaaactaaat
     1681 cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg gctctattcc ccatcaaatt
     1741 cacttgggtg agctgcatgc tattttgaga agacaagaag acttttatcc atttttaaaa
     1801 gacaatcgtg agaagattga aaaaatcttg acttttcgaa ttccttatta tgttggtcca
     1861 ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga agtctgaaga aacaattacc
     1921 ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag ctcaatcatt tattgaacgc
     1981 atgacaaact ttgataaaaa tcttccaaat gaaaaagtac taccaaaaca tagtttgctt
     2041 tatgagtatt ttacggttta taacgaattg acaaaggtca aatatgttac tgaaggaatg
     2101 cgaaaaccag catttctttc aggtgaacag aagaaagcca ttgttgattt actcttcaaa
     2161 acaaatcgaa aagtaaccgt taagcaatta aaagaagatt atttcaaaaa aatagaatgt
     2221 tttgatagtg ttgaaatttc aggagttgaa gatagattta atgcttcatt aggtacctac
     2281 catgatttgc taaaaattat taaagataaa gattttttgg ataatgaaga aaatgaagat
     2341 atcttagagg atattgtttt aacattgacc ttatttgaag atagggagat gattgaggaa
     2401 agacttaaaa catatgctca cctctttgat gataaggtga tgaaacagct taaacgtcgc
     2461 cgttatactg gttggggacg tttgtctcga aaattgatta atggtattag ggataagcaa
     2521 tctggcaaaa caatattaga ttttttgaaa tcagatggtt ttgccaatcg caattttatg
     2581 cagctgatcc atgatgatag tttgacattt aaagaagaca ttcaaaaagc acaagtgtct
     2641 ggacaaggcg atagtttaca tgaacatatt gcaaatttag ctggtagccc tgctattaaa
     2701 aaaggtattt tacagactgt aaaagttgtt gatgaattgg tcaaagtaat ggggcggcat
     2761 aagccagaaa atatcgttat tgaaatggca cgtgaaaatc agacaactca aaagggccag
     2821 aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta tcaaagaatt aggaagtcag
     2881 attcttaaag agcatcctgt tgaaaatact caattgcaaa atgaaaagct ctatctctat
     2941 tatctccaaa atggaagaga catgtatgtg gaccaagaat tagatattaa tcgtttaagt
     3001 gattatgatg tcgatcacat tgttccacaa agtttcctta aagacgattc aatagacaat
     3061 aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg ataacgttcc aagtgaagaa
     3121 gtagtcaaaa agatgaaaaa ctattggaga caacttctaa acgccaagtt aatcactcaa
     3181 cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt tgagtgaact tgataaagct
     3241 ggttttatca aacgccaatt ggttgaaact cgccaaatca ctaagcatgt ggcacaaatt
     3301 ttggatagtc gcatgaatac taaatacgat gaaaatgata aacttattcg agaggttaaa
     3361 gtgattacct taaaatctaa attagtttct gacttccgaa aagatttcca attctataaa
     3421 gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt atctaaatgc cgtcgttgga
     3481 actgctttga ttaagaaata tccaaaactt gaatcggagt ttgtctatgg tgattataaa
     3541 gtttatgatg ttcgtaaaat gattgctaag tctgagcaag aaataggcaa agcaaccgca
     3601 aaatatttct tttactctaa tatcatgaac ttcttcaaaa cagaaattac acttgcaaat
     3661 ggagagattc gcaaacgccc tctaatcgaa actaatgggg aaactggaga aattgtctgg
     3721 gataaagggc gagattttgc cacagtgcgc aaagtattgt ccatgcccca agtcaatatt
     3781 gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg agtcaatttt accaaaaaga
     3841 aattcggaca agcttattgc tcgtaaaaaa gactgggatc caaaaaaata tggtggtttt
     3901 gatagtccaa cggtagctta ttcagtccta gtggttgcta aggtggaaaa agggaaatcg
     3961 aagaagttaa aatccgttaa agagttacta gggatcacaa ttatggaaag aagttccttt
     4021 gaaaaaaatc cgattgactt tttagaagct aaaggatata aggaagttaa aaaagactta
     4081 atcattaaac tacctaaata tagtcttttt gagttagaaa acggtcgtaa acggatgctg
     4141 gctagtgccg gagaattaca aaaaggaaat gagctggctc tgccaagcaa atatgtgaat
     4201 tttttatatt tagctagtca ttatgaaaag ttgaagggta gtccagaaga taacgaacaa
     4261 aaacaattgt ttgtggagca gcataagcat tatttagatg agattattga gcaaatcagt
     4321 gaattttcta agcgtgttat tttagcagat gccaatttag ataaagttct tagtgcatat
     4381 aacaaacata gagacaaacc aatacgtgaa caagcagaaa atattattca tttatttacg
     4441 ttgacgaatc ttggagctcc cgctgctttt aaatattttg atacaacaat tgatcgtaaa
     4501 cgatatacgt ctacaaaaga agttttagat gccactctta tccatcaatc catcactggt
     4561 ctttatgaaa cacgcattga tttgagtcag ctaggaggtg actgaagtat attttagatg
     4621 aagattattt cttaatctag attctactgg tattggcaca aacctgattc caatttgagc
     4681 aaggctatgt gccatctcga tactcgttct taactcaaca gaagatgctt tgtgcataca
     4741 gcccctcgtt tattatttat ctcctcagcc agccgctgtg ctttcagtgg atttcggata
     4801 acagaaaggc cgggaaatac ccagcctcgc tttgtaacgg agtagacgaa agtgattgcg
     4861 cctacccgga tattatcgtg aggatgcgtc atcgccattg ctccccaaat acaaaaccaa
     4921 tttcagccag tgcctcgtcc attttttcga tgaactccgg cacgatctcg tcaaaactcg
     4981 ccatgtactt ttcatcccgc tcaatcacga cataatgcag gccttcacgc ttcatacgcg
     5041 ggtcatagtt ggcaaagtac caggcatttt ttcgcgtcac ccacatgctg tactgcacct
     5101 gggccatgta agctgacttt atggcctcga aaccaccgag ccggaacttc atgaaatccc
     5161 gggaggtaaa cgggcatttc agttcaaggc cgttgccgtc actgcataaa ccatcgggag
     5221 agcaggcggt acgcatactt tcgtcgcgat agatgatcgg ggattcagta acattcacgc
     5281 cggaagtgaa ttcaaacagg gttctggcgt cgttctcgta ctgttttccc caggccagtg
     5341 ctttagcgtt aacttccgga gccacaccgg tgcaaacctc agcaagcagg gtgtggaagt
     5401 aggacatttt catgtcaggc cacttctttc cggagcgggg ttttgctatc acgttgtgaa
     5461 cttctgaagc ggtgatgacg ccgagccgta atttgtgcca cgcatcatcc ccctgttcga
     5521 cagctctcac atcgatcccg gtacgctgca ggataatgtc cggtgtcatg ctgccacctt
     5581 ctgctctgcg gctttctgtt tcaggaatcc aagagctttt actgcttcgg cctgtgtcag
     5641 ttctgacgat gcacgaatgt cgcggcgaaa tatctgggaa cagagcggca ataagtcgtc
     5701 atcccatgtt ttatccaggg cgatcagcag agtgttaatc tcctgcatgg tttcatcgtt
     5761 aaccggagtg atgtcgcgtt ccggctgacg ttctgcagtg tatgcagtat tttcgacaat
     5821 gcgctcggct tcatccttgt catagatacc agcaaatccg aaggccagac gggcacactg
     5881 aatcatggct ttatgacgta acatccgttt gggatgcgac tgccacggcc ccgtgatttc
     5941 tctgccttcg cgagttttga atggttcgcg gcggcattca tccatccatt cggtaacgca
     6001 gatcggatga ttacggtcct tgcggtaaat ccggcatgta caggattcat tgtcctgctc
     6061 aaagtccatg ccatcaaact gctggttttc attgatgatg cgggaccagc catcaacgcc
     6121 caccaccgga acgatgccat tctgcttatc aggaaaggcg taaatttctt tcgtccacgg
     6181 attaaggccg tactggttgg caacgatcag taatgcgatg aactgcgcat cgctggcatc
     6241 acctttaaat gccgtctggc gaagagtggt gatcagttcc tgtgggtcga cagaatccat
     6301 gccgacacgt tcagccagct tcccagccag cgttgcgagt gcagtactca ttcgttttat
     6361 acctctgaat caatatcaac ctggtggtga gcaatggttt caaccatgta ccggatgtgt
     6421 tctgccatgc gctcctgaaa ctcaacatcg tcatcaaacg cacgggtaat ggattttttg
     6481 ctggccccgt ggcgttgcaa atgatcgatg catagcgatt caaacaggtg ctggggcagg
     6541 cctttttcca tgtcgtctgc cagttctgcc tctttctctt cacgggcgag ctgctggtag
     6601 tgacgcgccc agctctgagc ctcaagacga tcctgaatgt aataagcgtt catggctgaa
     6661 ctcctgaaat agctgtgaaa atatcgcccg cgaaatgccg ggctgattag gaaaacagga
     6721 aagggggtta gtgaatgctt ttgcttgatc tcagtttcag tattaatatc cattttttat
     6781 aacctcctta gagctcgaat tcccaaaaaa acgggtatgg agaaacagta gagagttgcg
     6841 ataaaaagcg tcaggtagga tccgctaatc ttatggataa aaatgctatg gcatagcaaa
     6901 gtgtgacgcc gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt
     6961 tacgcgtttt tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg
     7021 ttaccggttt ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc
     7081 aatatggaca attggtttct tctctgaatg gcgggagtat gaaaagtatg gctgaagcgc
     7141 aaaatgatcc cctgctgccg ggatactcgt ttaatgccca tctggtggcg ggtttaacgc
     7201 cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt
     7261 atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg
     7321 tttgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc
     7381 atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc
     7441 atgaatggct taactggccg tcaatatttg ccaatacggg gttctttcgc ccggatgaag
     7501 cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag
     7561 ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg cggcacatgg
     7621 aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt
     7681 acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt
     7741 gcttgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa
     7801 gctggcgcga ggaccaacgt atcagccagg cgaagctgct tttgagcacc acccggatgc
     7861 ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctacttc tcgcgggtat
     7921 ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga
     7981 atgatgtagc cgtcaagttg tcataataaa tcgatgcagg tggcactttt cggggaaatg
     8041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatgt
     8101 ctagaggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
     8161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8281 ggcgaatggc gccttgacaa ttaatcatcg gctcgtataa tgtgtggagt gaaggatacg
     8341 tgtgcgagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     8401 aaaaagtggc accgagtcgg tgcttttggc gcctgatgcg gtattttctc cttacgcatc
     8461 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     8521 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     8581 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     8641 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct attattaacg cttacaattt
     8701 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tcaggtggca
     8761 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     8821 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac gtgaggaggg
     8881 ccaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
     8941 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
     9001 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
     9061 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
     9121 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     9181 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     9241 tggccgagga gcaagactga cacgtgctaa aacttcattt ttaatttaaa aggatctagg
     9301 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9361 gagcgtcaga cagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9421 ggtttcggtc tcgcggtggt ctaccgtatt ccatcgcgcc ggatacgcag aaacatgtgc
     9481 cgatggcgct gtggctctcc gccgactacc agcagcgcta cggcatttcc gcgcactgtc
     9541 tgcagcagcg ggcgcaaaaa gagaattact cgcaggataa tctgttctcc accctgctcg
     9601 gcctgctcgg cgtcagcacc cgcgagtacc aggcggcaga tgatatctta acgccatgta
     9661 gagaggctgg ttgtctagaa ttacagcctg atcgtgctgg atctcggcct gccggatgaa
     9721 gacgggctgc attttctctc ccgcatgcgg cgggagaaaa tgacccagcc ggtgctgatc
     9781 ctcaccgccc gcgatacgct tgaggatcgc attagcggtc tcgataccgg ggcggatgac
     9841 tacctggtaa aaccctttgc cctggaggaa ctcaatgcgc gcattcgcgc cctgctccgc
     9901 cggcataaca accagggcga taatgaaatc agcgtcggca atctgcgcct taacgtcact
     9961 cgccgcctgg tgtggctggg tgaaacggcg ctggatctta cgccgaaaga gtatgccctg
    10021 ctgtcgcggc tgatgatgaa agccggtagc ccggtgcatc gcgagatcct ctacaacgac
    10081 atttacagct gggacaacga gccggcgacc aataccctgg aagtgcatat tcacaaccta
    10141 cgcgaaaaga ttggcaaatc gcggatccgc accgtccggg gctttggcta tatgctgaca
    10201 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
    10261 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
    10321 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
    10381 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    10441 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    10501 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    10561 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
    10621 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    10681 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    10741 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    10801 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    10861 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    10921 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    10981 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    11041 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    11101 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    11161 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    11221 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    11281 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa t
//
