LOCUS       sequence-32422-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL4DBD
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(299..332)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(517..535)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 ggcgcgcctc tagatcgata ccgtcgacta aagccaaata gaaaattatt cagttcctgg
       61 cttaagtttt taaaagtgat attatttatt tggttgtaac caaccaaaag aatgtaaata
      121 actaatacat aattatgtta gttttaagtt agcaacaaat tgattttagc tatattagct
      181 acttggttaa taaatagaat atatttattt aaagataatt gcgtttttat tgtcagggag
      241 tgagtttgct taaaaactcg tttagatcca ctagcttaat taagcatgcc taggctagat
      301 aacttcgtat agcatacatt atacgaagtt atagcttctg atggaattag aacttggcaa
      361 aacaatactg agaatgaagt gtatgtggaa cagaggctgc tgatctcgtt cttcaggcta
      421 tgaaactgac acatttggaa accacagtac ttagaaccac aaagtgggaa tcaagagaaa
      481 aacaatgatc ccacgagaga tcgaattcta gtatgtatgt aagttaataa aacccatttt
      541 tgcggaaagt agataaaaaa aacatttttt ttttttactg cactggatat cattgaactt
      601 atctgatcag ttttaaattt acttcgatcc aagggtattt gatgtaccag gttctttcga
      661 ttacctctca ctcaaaatga cattccactc aaagtcagcg ctgtttgcct ccttctctgt
      721 ccacagaaat atcgccgtct ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg
      781 tagcgttacg tagcgtcaat gtccgccttc agttgcattt tgtcagcggt ttcgtgacga
      841 agctcc
//
