LOCUS       sequence-324220-       12920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encoding the Kandolf strain of CVB3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12920)
  AUTHORS   Lopacinski AB, Sweatt AJ, Smolko CM, Gray-Gaillard E, Borgman CA, 
            Shah M, Janes KA
  TITLE     Modeling the complete kinetics of coxsackievirus B3 reveals human 
            determinants of host-cell feedback.
  JOURNAL   Cell Syst. 2021 Apr 21;12(4):304-323.e13. doi: 
            10.1016/j.cels.2021.02.004. Epub 2021 Mar 18.
  PUBMED    33740397
REFERENCE   2  (bases 1 to 12920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2021.02.004"; journalName: "Cell Syst"; date: 
            "2021-04-21- 21"; volume: "12"; issue: "4"; pages: "304-323.e13"
COMMENT     Sequence Label: pcDNA CVB3
FEATURES             Location/Qualifiers
     source          1..12920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8473..8491)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8474..8491)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8511..8528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8517..8741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8787..9215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8874..8893)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9084..9105
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9224..9244)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9229..9558
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9409..9544
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9471..9490
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             9625..10419
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9679..9698)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10289..10308
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10593..10714
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10630..10649)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10684..10703
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10763..10779)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10763..10779)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10776..10798)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10787..10803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10811..10817,10818..10835,10836..10841))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10856..10877
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10994..11011)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11165..11753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11245..11264)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11924..12715,12716..12784))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12547..12566
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12785..12889)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttctccaaga catccccccc
      961 ccaaaacagc ctgtgggttg atcccaccca cagggcccat tgggcgctag cactctggta
     1021 tcacggtacc tttgtgcgcc tgttttatac cccctccccc aactgtaact tagaagtaac
     1081 acacaccgat caacagtcag cgtggcacac cagccacgtt ttgatcaagc acttctgtta
     1141 ccccggactg agtatcaata gactgctcac gcggttgaag gagaaagcgt tcgttatccg
     1201 gccaactact tcgaaaaacc tagtaacacc gtggaagttg cagagtgttt cgctcagcac
     1261 taccccagtg tagatcaggt cgatgagtca ccgcattccc cacgggcgac cgtggcggtg
     1321 gctgcgttgg cggcctgccc atggggaaac ccatgggacg ctctaataca gacatggtgc
     1381 gaagagtcta ttgagctagt tggtagtcct ccggcccctg aatgcggcta atcctaactg
     1441 cggagcacac accctcaagc cagagggcag tgtgtcgtaa cgggcaactc tgcagcggaa
     1501 ccgactactt tgtgtgtccg tgtttcattt tattcctata ctggctgctt atggtgacaa
     1561 ttgagagatc gttaccatat agctattgga ttggccatcc ggtgactaat agagctatta
     1621 tatatccctt tgttgggttt ataccactta gcttgaaaga ggttaaaaca ttacaattca
     1681 ttgttaagtt gaatacagca aaatgggagc tcaagtatca acgcaaaaga ctggggcaca
     1741 tgagaccagg ctgaatgcta gcggcaattc catcattcac tacacaaata ttaattatta
     1801 caaggatgcc gcatccaact cagccaatcg gcaggatttc actcaagacc cgggcaagtt
     1861 cacagaacca gtgaaagata tcatgattaa atcactacca gctctcaact cccccacagt
     1921 agaggagtgc ggatacagtg acagggcgag atcaatcaca ttaggtaact ccaccataac
     1981 gactcaggaa tgcgccaacg tggtggtggg ctatggagta tggccagatt atctaaagga
     2041 tagtgaggca acagcagagg accaaccgac ccaaccagac gttgccacat gtaggttcta
     2101 tacccttgac tctgtgcaat ggcagaaaac ctcaccagga tggtggtgga agctgcccga
     2161 tgctttgtcg aacttaggac tgtttgggca gaacatgcag taccactact taggccgaac
     2221 tgggtatacc gtacatgtgc agtgcaatgc atctaagttc caccaaggat gcttgctagt
     2281 agtgtgtgta ccggaagctg agatgggttg cgcaacgcta gacaacaccc catccagtgc
     2341 agaattgctg gggggcgata gcgcaaagga gtttgcggac aaaccggtcg catccgggtc
     2401 caacaagttg gtacagaggg tggtgtataa tgcaggcatg ggggtgggtg ttggaaacct
     2461 caccattttc ccccaccaat ggatcaacct acgcaccaat aatagtgcta caattgtgat
     2521 gccatacacc aacagtgtac ctatggataa catgtttagg cataacaacg tcaccctaat
     2581 ggttatccca tttgtaccgc tagattactg ccctgggtcc accacgtacg tcccaattac
     2641 ggtcacgata gccccaatgt gtgccgagta caatgggtta cgtttagcag ggcaccaggg
     2701 cttaccaacc atgaatactc cggggagctg tcaatttctg acatcagacg acttccaatc
     2761 accatccgcc atgccgcaat atgacgtcac accagagatg aggatacctg gtgaggtgaa
     2821 aaacttgatg gaaatagctg aggttgactc agttgtccca gtccaaaatg ttggagagaa
     2881 ggtcaactct atggaagcat accagatacc tgtgagatcc aacgaaggat ctggaacgca
     2941 agtattcggc tttccactgc aaccagggta ctcgagtgtt tttagtcgga cgctcctagg
     3001 agagatcttg aactattata cacattggtc aggcagcata aagcttacgt ttatgttctg
     3061 tggttcggcc atggctactg gaaaattcct tttggcatac tcaccaccag gtgctggagc
     3121 tcctacaaaa agggttgatg ctatgcttgg tactcatgta atttgggacg tggggctaca
     3181 atcaagttgc gtgctgtgta taccctggat aagccaaaca cactaccggt ttgttgcttc
     3241 agatgagtat accgcagggg gttttattac gtgctggtat caaacaaaca tagtggtccc
     3301 agcggatgcc caaagctcct gttacatcat gtgtttcgtg tcagcatgca atgacttctc
     3361 tgtcaggcta ttgaaggaca ctcctttcat ttcgcagcaa aactttttcc agggcccagt
     3421 ggaagacgcg ataacagccg ctatagggag agttgcggat accgtgggta cagggccaac
     3481 caactcagaa gctataccag cactcactgc tgctgagacg ggtcacacgt cacaagtagt
     3541 gccgggtgac actatgcaga cacgccacgt taagaactac cattcaaggt ccgagtcaac
     3601 catagagaac ttcctatgta ggtcagcatg cgtgtacttt acggagtata aaaactcagg
     3661 tgccaagcgg tatgctgaat gggtattaac accacgacaa gcagcacaac ttaggagaaa
     3721 gctagaattc tttacctacg tccggttcga cctggagctg acgtttgtca taacaagtac
     3781 tcaacagccc tcaaccacac agaaccaaga tgcacagatc ctaacacacc aaattatgta
     3841 tgtaccacca ggtggacctg taccagataa agttgattca tacgtgtggc aaacatctac
     3901 gaatcccagt gtgttttgga ccgagggaaa cgccccgccg cgcatgtcca taccgttttt
     3961 gagcattggc aacgcctatt caaatttcta tgacggatgg tctgaatttt ccaggaacgg
     4021 agtttacggc atcaacacgc taaacaacat gggcacgcta tatgcaagac atgtcaacgc
     4081 tggaagcacg ggtccaataa aaagcaccat tagaatctac ttcaaaccga agcatgtcaa
     4141 agcgtggata cctagaccac ctagactctg ccaatacgag aaggcaaaga acgtgaactt
     4201 ccaacccagc ggagttacca ctactaggca aagcatcact acaatgacaa atacgggcgc
     4261 atttggacaa caatcagggg cagtgtatgt ggggaactac agggtggtaa atagacatct
     4321 agctaccagt gctgactggc aaaactgtgt gtgggaaagt tacaacagag acctcttagt
     4381 gagcacgacc acagcacatg gatgtgatat tatagccaga tgtcagtgca caacgggagt
     4441 gtacttttgt gcgtccaaaa acaagcacta cccaatttcg tttgaaggac caggtctagt
     4501 agaggtccaa gagagtgaat actaccccag gagataccaa tcccatgtgc ttttagcagc
     4561 tggattttcc gaaccaggtg actgtggcgg tatcctaagg tgtgagcatg gtgtcattgg
     4621 cattgtgacc atggggggtg aaggcgtggt cggatttgca gacatccgtg atctcctgtg
     4681 gctggaagat gatgcaatgg aacagggagt gaaggactat gtggaacagc ttggaaatgc
     4741 attcggctcc ggctttacta accaaatatg tgagcaagtc aacctcctga aagaatcact
     4801 agtgggtcaa gactccatct tagagaaatc tctaaaagcc ttagttaaga taatatcagc
     4861 cttagtaatt gtggtgagga accacgatga cctgatcact gtgactgcca cactagccct
     4921 tatcggttgt acctcgtccc cgtggcggtg gctcaaacag aaggtgtcac aatattacgg
     4981 aatccctatg gctgaacgcc aaaacaatag ctggcttaag aaatttactg aaatgacaaa
     5041 tgcttgcaag ggtatggaat ggatagctgt caaaattcag aaattcattg aatggctcaa
     5101 agtaaaaatt ttgccagagg tcagagaaaa acacgagttc ctgaacagac ttaaacaact
     5161 ccccttatta gaaagtcaga tcgccacaat cgagcagagc gcgccatccc aaagtgacca
     5221 ggaacaatta ttttccaatg tccaatactt tgcccactat tgcagaaagt acgctcccct
     5281 ctacgcagct gaagcaaaga gggtgttctc ccttgagaag aagatgagca attacataca
     5341 gttcaagtcc aaatgccgta ttgaacctgt atgtttgctc ctgcacggga gccctggtgc
     5401 cggcaagtcg gtggcaacaa acttaattgg aaggtcgctt gctgagaaac tcaacagctc
     5461 agtgtactca ctaccgccag acccagatca cttcgacgga tacaaacagc aggccgtggt
     5521 gattatggac gatctatgcc agaatcctga tgggaaagac gtctccttgt tctgccaaat
     5581 ggtttccagt gtagattttg taccacccat ggctgcccta gaagagaaag gcattctgtt
     5641 cacctcaccg tttgtcttgg catcgaccaa tgcaggatct attaatgctc caaccgtgtc
     5701 agatagcaga gccttggcaa ggagatttca ctttgacatg aacatcgagg ttatttccat
     5761 gtacagtcag aatggcaaga taaacatgcc catgtcagtc aagacttgtg acgatgagtg
     5821 ttgcccggtc aattttaaaa agtgctgccc tcttgtgtgt gggaaggcta tacaattcat
     5881 tgatagaaga acacaggtca gatactctct agacatgcta gtcaccgaga tgtttaggga
     5941 gtacaatcat agacatagcg tggggaccac gcttgaggca ctgttccagg gaccaccagt
     6001 atacagagag atcaaaatta gcgttgcacc agagacacca ccaccgcccg ccattgcgga
     6061 cctgctcaaa tcggtagaca gtgaggctgt gagggagtac tgcaaagaaa aaggatggtt
     6121 ggttcctgag atcaactcca ccctccaaat tgagaaacat gtcagtcggg ctttcatttg
     6181 cttacaggca ttgaccacat ttgtgtcagt ggctggaatc atatatataa tatataagct
     6241 ctttgcgggt tttcaaggtg cttatacagg agtgcccaac cagaagccca gagtgcctac
     6301 cctgaggcaa gcaaaagtgc aaggccctgc ctttgagttc gccgtcgcaa tgatgaaaag
     6361 gaactcaagc acggtgaaaa ctgaatatgg cgagtttacc atgctgggca tctatgacag
     6421 gtgggccgtt ttgccacgcc acgccaaacc tgggccaacc atcttgatga atgatcaaga
     6481 ggttggtgtg ctagatgcca aggagctagt agacaaggac ggcaccaact tagaactgac
     6541 actactcaaa ttgaaccgga atgagaagtt cagagacatc agaggcttct tagccaagga
     6601 ggaagtggag gttaatgagg cagtgctagc aattaacacc agcaagtttc ccaacatgta
     6661 cattccagta ggacaggtca cagaatacgg cttcctaaac ctaggtggca cacccaccaa
     6721 gagaatgctt atgtacaact tccccacaag agcaggccag tgtggtggag tgctcatgtc
     6781 caccggcaag gtactgggta tccatgttgg tggaaatggc catcagggct tctcagcagc
     6841 actcctcaaa cactacttca atgatgagca aggtgaaata gaatttattg agagctcaaa
     6901 ggacgccggg tttccagtca tcaacacacc aagtaaaaca aagttggagc ctagtgtttt
     6961 ccaccaggtc tttgagggga acaaagaacc agcagtactc aggagtgggg atccacgtct
     7021 caaggccaat tttgaagagg ctatattttc caagtatata ggaaatgtca acacacacgt
     7081 ggatgagtac atgctggaag cagtggacca ctacgcaggc caactagcca ccctagatat
     7141 cagcactgaa ccaatgaaac tggaggacgc agtgtacggt accgagggtc ttgaggcgct
     7201 tgatctaaca acgagtgccg gttacccata tgttgcactg ggtatcaaga agagggacat
     7261 cctctctaag aagactaagg acctaacaaa gttaaaggaa tgtatggaca agtatggcct
     7321 gaacctacca atggtgactt atgtaaaaga tgagctcagg tccatagaga aggtagcgaa
     7381 aggaaagtct aggctgattg aggcgtccag tttgaatgat tcagtggcga tgagacagac
     7441 atttggtaat ctgtacaaaa ctttccacct aaacccaggg gttgtgactg gtagtgctgt
     7501 tgggtgtgac ccagacctct tttggagcaa gataccagtg atgttagatg gacatctcat
     7561 agcatttgat tactctgggt acgatgctag cttaagccct gtctggtttg cttgcctaaa
     7621 aatgttactt gagaagcttg gatacacgca caaagagaca aactacattg actacttgtg
     7681 caactcccat cacctgtaca gggataaaca ttactttgtg aggggtggca tgccctcggg
     7741 atgttctggt accagtattt tcaactcaat gattaacaat atcataatta ggacactaat
     7801 gctaaaagtg tacaaaggga ttgacttgga ccaattcagg atgatcgcat atggtgatga
     7861 tgtgatcgca tcgtacccat ggcctataga tgcatcttta ctcgctgaag ctggtaaggg
     7921 ttacgggctg atcatgacac cagcagataa gggagagtgc tttaacgaag ttacctggac
     7981 caacgccact ttcctaaaga ggtattttag agcagatgaa cagtacccct tcctggtgca
     8041 tcctgttatg cccatgaaag acatacacga atcaattaga tggaccaagg atccaaagaa
     8101 cacccaagat cacgtgcgct cactgtgtct attagcttgg cataacgggg agcacgaata
     8161 tgaggagttc atccgtaaaa ttagaagcgt cccagtcgga cgttgtttga ccctccccgc
     8221 gttttcaact ctacgcagga agtggttgga ctccttttag attagagaca atttgaaata
     8281 atttagattg gcttaaccct actgtgctaa ccgaaccaga taacggtaca gtaggggtaa
     8341 attctccgca ttcggtgcgg aaaaaaaaaa aaaaaaaaaa aarggggggg gggggggggg
     8401 gggtagactc gagaattctg cagatatcca tcacactggc ggccgctcga gcatgcatct
     8461 agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag cctcgactgt
     8521 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     8581 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     8641 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     8701 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     8761 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     8821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     8881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9001 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9061 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9121 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9181 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     9241 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9301 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9361 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9421 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9481 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9541 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
     9601 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
     9661 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     9721 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     9781 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     9841 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     9901 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     9961 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
    10021 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
    10081 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
    10141 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
    10201 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
    10261 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
    10321 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
    10381 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
    10441 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
    10501 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
    10561 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
    10621 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    10681 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
    10741 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10801 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10861 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10921 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
    10981 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
    11041 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
    11101 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggca gcgttgctgg
    11161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
    11221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
    11281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
    11341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
    11401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
    11461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
    11521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
    11581 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
    11641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
    11701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
    11761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
    11821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
    11881 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
    11941 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
    12001 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
    12061 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
    12121 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
    12181 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
    12241 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
    12301 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
    12361 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
    12421 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
    12481 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
    12541 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
    12601 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
    12661 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
    12721 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
    12781 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
    12841 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
    12901 gaaaagtgcc acctgacgtc
//
