LOCUS       sequence-324239-        6270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tests for the impact of 1 uracil in conjunction with a hairpin 
            structure in a poly-uracil tract on human polymerase III 
            transcription from a GLN tRNA promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Verosloff MS, Corcoran WK, Dolberg TB, Bushhouse DZ, Leonard JN, 
            Lucks JB
  TITLE     RNA Sequence and Structure Determinants of Pol III Transcriptional 
            Termination in Human Cells.
  JOURNAL   J Mol Biol. 2021 Jun 25;433(13):166978. doi: 
            10.1016/j.jmb.2021.166978. Epub 2021 Apr 1.
  PUBMED    33811918
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol 
            Biol."; date: "2021-06-25"; volume: "433(13):166978. doi"; pages: " 
            10.1016/j.jmb.2021.166978. Epub 2021 Apr 1"
COMMENT     Sequence Label: GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt
FEATURES             Location/Qualifiers
     source          1..6270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     605..624
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1035..1364)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1049..1184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1349..1369
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    1909..2030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   2079..2208
                     /label=AAV2 ITR
     rep_origin      complement(2339..2767)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2449..2470)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2661..2680
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2794..2898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2899..2967,2968..3759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3117..3136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3930..4518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4419..4438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4961..5095
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5520..5540)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5670..5735
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(join(6267..6270,1..16))
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg gccacagtcg
       61 atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc atcgccatgg
      121 gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa cagttcggct
      181 ggcgcgagcc cctgatgctc ttgtccagat catcctgatc gacaagaccg gcttccatcc
      241 gagtacgtgc tcgctcgatg cgatgttcgc ttggtggtcg aatgggcagg tagccggatc
      301 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
      361 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
      421 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
      481 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
      541 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
      601 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
      661 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc
      721 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc
      781 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc
      841 ccagtctagc tatcggcatg taagcccact gcaagctacc tgctttctct ttgcgcttgc
      901 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg
      961 cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg agtgcttgcg
     1021 gcagcgtgaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     1081 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     1141 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     1201 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     1261 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     1321 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagccggatc
     1381 caaggtcggg caggaagtct ctcgtcggtt ccatggtgta atggttagca ctctggactc
     1441 tgaatccagc gatccgagtt caaatctcgg tggaacctat atactagcag ccaactccaa
     1501 ggttgcggct ttctgctgca atctcgacgg ccatcagtcg ccggtcccaa gcccggataa
     1561 aatgggaggg ggcgggaaac cgcctaacca tgccgactga tggcaggccc ggatagctca
     1621 gtcggtagag cagcggccgc gcgattcgag gaaggaggtc tgaggaggtc actgaatcgc
     1681 gcggccgcgg gtccagggtt caagtccctg ttcgggcctg ccatcagtcg gcgtggactg
     1741 tagaacactg ccaatgccgg tcccaagccc ggataaaagt ggagggtaca gtccacgctc
     1801 tagagcggac ttcggtccgc tttttactag gacctgcagg catgcaagct tgacgtcggt
     1861 taccgatatc catatggcgg ccgcatcgat ctcgagccgc ggactagtaa cttgtttatt
     1921 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1981 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctta
     2041 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
     2101 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     2161 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     2221 caaagatctc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2281 cagcctgaat ggctaatggg aaattgtaaa cgttaatatt ttgttaatat tttgttaaaa
     2341 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2401 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2461 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2521 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2581 aaagcactaa atcggaaccc taaagggatg ccccgattta gagcttgacg gggaaagccg
     2641 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2701 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2761 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     4081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4261 tgcaacacag ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4321 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4681 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4741 attcattaat gcagagatct ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4801 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     4861 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctggagg ggtggagtcg
     4921 tgacgtgaat tacgtcatag ggttagggag gtcctggatc gatccagaca tgataagata
     4981 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     5041 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     5101 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     5161 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac
     5221 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga
     5281 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta
     5341 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg
     5401 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta
     5461 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct
     5521 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca
     5581 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc
     5641 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt
     5701 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt
     5761 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat ccgggaccaa
     5821 agcggccatc gtgcctcccc actcctgcag ttcgggggca tggatgcgcg gatagccgct
     5881 gctggtttcc tggatgccga cggatttgca ctgccggtag aactccgcga ggtcgtccag
     5941 cctcaggcag cagctgaacc aactcgcgag gggatcgagc ccggggtggg cgaagaactc
     6001 cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa cgattccgaa
     6061 gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtgatggca ggttgggcgt
     6121 cgcttggtcg gtcatttcga accccagagt cccgctcaga agaactcgtc aagaaggcga
     6181 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     6241 gcccattcgc cgccaagctc ttcagcaata
//
