LOCUS       sequence-32425-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-GAL80
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(556..589)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(774..792)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 aatcatggaa gtatatcatt tacgaaatta taatgccatt gtgggtaata ttcatcgact
       61 gtatcaatct atctctgact tccacttcaa tacaaagaaa attcctgaat taccctcaca
      121 atttgtaatg caaggtttcg atttcgaagg ctttcccacc ttgatggatg ctctgatatt
      181 acacaggtta atcgagagcg tttataaaag taacatgatg ggctccacat taaacgttag
      241 caatatctcg cattatagtt tataatctag atcgataccg tcgactaaag ccaaatagaa
      301 aattattcag ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa
      361 ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga
      421 ttttagctat attagctact tggttaataa atagaatata tttatttaaa gataattgcg
      481 tttttattgt cagggagtga gtttgcttaa aaactcgttt agatccacta gcttaattaa
      541 gcatgcctag gctagataac ttcgtatagc atacattata cgaagttata gcttctgatg
      601 gaattagaac ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga
      661 tctcgttctt caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa
      721 gtgggaatca agagaaaaac aatgatccca cgagagatcg aattctagta tgtatgtaag
      781 ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac
      841 tggatatcat tgaacttatc tgatcag
//
