LOCUS       sequence-324276-        1210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of CSL F235R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Bottoni G, Katarkar A, Tassone B, Ghosh S, Clocchiatti A, Goruppi S,
            Bordignon P, Jafari P, Tordini F, Lunardi T, Hoetzenecker W, Neel V,
            Lingner J, Paolo Dotto G
  TITLE     CSL controls telomere maintenance and genome stability in human 
            dermal fibroblasts.
  JOURNAL   Nat Commun. 2019 Aug 29;10(1):3884. doi: 10.1038/s41467-019-11785-7.
  PUBMED    31467287
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-29"; volume: "10(1):3884. doi"; pages: " 
            10.1038/s41467-019-11785-7"
COMMENT     Sequence Label: pcDNA3. Flag-CSL F235R
FEATURES             Location/Qualifiers
     source          1..1210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccatg gactacaaag acgatgacga taaaggaatt
      121 ctgcagatgc ctgttgtgac agggaaattt ggtgagcggc ctccacctaa acgacttact
      181 agggaagcta tgcgaaatta tttaaaagag cgaggggatc aaacagtact tattcttcat
      241 gcaaaagttg cacagaagtc atatggaaat gaaaaaaggt ttttttgccc acctccttgt
      301 gtatatctta tgggcagtgg atggaagaaa aaaaaagaac aaatggaacg cgatggttgt
      361 tctgaacaag agtctcaacc gtgtgcattt attgggatag gaaatagtga ccaagaaatg
      421 cagcagctaa acttggaagg aaagaactat tgcacagcca aaacattgta tatatctgac
      481 tcagacaagc gaaagcactt catgttgtct gtaaagatgt tctatggcaa cagtgatgac
      541 attggtgtgt tcctcagcaa gcggataaaa gtcatctcca aaccttccaa aaagaagcag
      601 tcattgaaaa atgctgactt atgcattgcc tcaggaacaa aggtggctct gtttaatcga
      661 ctacgatccc agacagttag taccagatac ttgcatgtag aaggaggtaa ttttcatgcc
      721 agttcacagc agtggggagc cttttttatt catctcttgg atgatgatga atcagaagga
      781 gaagaacgca cagtccgaga tggctacatc cattatggac aaacagtcaa acttgtgtgc
      841 tcagttactg gcatggcact cccaagattg ataattagga aagttgataa gcagaccgca
      901 ttattggatg cagatgatcc tgtgtcacaa ctccataaat gtgcatttta ccttaaggat
      961 acagaaagaa tgtatttgtg cctttctcaa gaaagaataa ttcaatttca ggccactcca
     1021 tgtccaaaag aaccaaataa agagatgata aatgatggcg cttcctggac aatcattagc
     1081 acagataagg cagagtatac attttatgag ggaatgggcc ctgtccttgc cccagtcmct
     1141 cctgkgcctg kggtagagng ccttcagttg aatggcggng gggacgtagc aatgcttgaa
     1201 cttannggam
//
