LOCUS       sequence-324283-        9284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9284)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 9284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight eGFP Hygro (w783-1)
FEATURES             Location/Qualifiers
     source          1..9284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    393..417
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    446..498
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      510..519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(516..518,519..521,522..1235)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(516..518,519..521,522..1232)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(561..582)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(822..841)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1169..1190
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1260..1284)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1315..1903
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1368..1388)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1786..1797)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1959..2446
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1994..2017)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2379..2398
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2464..3489
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             2464..3489
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             3863..4096
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4168..4289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4205..4224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4259..4278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4329..4464
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4484..4503)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4485..4503)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4513..4530)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4513..4529)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4522..4544)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4671..5126
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4758..4777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4968..4989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5152..5256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5257..5325,5326..6117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5475..5494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6288..6876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6777..6796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7030..7047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7164..7185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7200..7205,7206..7223,7224..7230)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7238..7254
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7243..7265
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7262..7278
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7262..7278
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7297..7317
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7299..7317
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7345..7571
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7572..7752
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7799..7924
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8417..8650
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8835..8879
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9146..9263
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattcgagct cggtacccgg ggatccaccg gttcgtcgac
      361 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttta
      421 aaggaaccaa ttcagtcgac tggatctcga gctcaagctt cgaattctgc agtcgacggt
      481 accgcgggcc cgggatccac cggccggtcg ccaccatggt gagcaagggc gaggagctgt
      541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      841 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      901 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
      961 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1021 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1081 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     1141 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1201 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcactcga gatatctaga
     1261 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa
     1321 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     1381 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     1441 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     1501 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     1561 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     1621 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     1681 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     1741 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     1801 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     1861 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata
     1921 tccggactag tgatctctcg aggttaacga attctaccgg gtaggggagg cgcttttccc
     1981 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     2041 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     2101 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     2161 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     2221 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     2281 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     2341 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     2401 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc
     2461 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     2521 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     2581 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     2641 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     2701 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     2761 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     2821 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     2881 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     2941 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     3001 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     3061 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     3121 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     3181 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     3241 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     3301 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     3361 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     3421 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     3481 aaggaataga gtagatgccg accgaacaag agctgatttc gagaacgcct cagccagcaa
     3541 ctcgcgcgag cctagcaagg caaatgcgag agaacggcct tacgcttggt ggcacagttc
     3601 tcgtccacag ttcgctaagc tcgctcggct gggtcgcggg agggccggtc gcagtgattc
     3661 aggcccttct ggattgtgtt ggtccccagg gcacgattgt catgcccacg cactcgggtg
     3721 atctgactga tcccgcagat tggagatcgc cgcccgtgcc tgccgattgg gtgcagatcc
     3781 gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3841 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     3901 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3961 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4021 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4081 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     4141 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4201 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4261 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4321 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4381 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4441 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     4501 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4561 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4621 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5101 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5161 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5221 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5281 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5341 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5401 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5461 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5521 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5581 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5641 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5701 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5761 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5821 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5881 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5941 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     6001 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6061 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6121 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6181 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6241 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6301 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6361 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6421 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6481 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6541 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6601 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6661 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6721 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6781 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6841 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6901 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6961 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7021 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7081 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7141 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7201 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7261 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     7321 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     7381 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     7441 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     7501 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     7561 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7621 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7681 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7741 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     7801 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     7861 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     7921 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     7981 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     8041 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     8101 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     8161 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     8221 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     8281 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     8341 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     8401 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     8461 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     8521 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     8581 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     8641 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     8701 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     8761 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     8821 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     8881 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     8941 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     9001 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     9061 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     9121 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
     9181 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9241 aaattacaaa aattcaaaat tttatcgatc acgagactag cctc
//
