LOCUS       sequence-32429-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-LexA
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..273
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(425..444)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    469..517
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ccggggatcc gtcgacctgc aggaattccc ggggatctgg cccccccgac cgatgtcagc
       61 ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc cgacgcgcta
      121 gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg atttaccccc
      181 cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga gcagatgttt
      241 accgatgccc ttggaattga cgagtacggt gggtaggggg cgcgaccgga cccgcatccc
      301 ccgtctgggt tttcccctcc cgtccccggt tcgtatccac aataaacacg agcacataca
      361 ttacaaaaac ctgcggttgt cgtctgatta tttggtggat ccgggggaga tgggggaggc
      421 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
      481 gacagaataa aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
      541 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
      601 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
      661 ggggcggcag gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg
      721 ggaatggttt atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg
      781 acccaagctt ctagatcgat accgtcgact aaagccaaat agaaaattat tcagttcctg
      841 gcttaagttt ttaaaagtga tattatttat
//
