LOCUS       sequence-324291-       10741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter of Agrobacterium rhizogenes mediated 
            transformed roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10741)
  AUTHORS   Lin MH, Gresshoff PM, Indrasumunar A, Ferguson BJ
  TITLE     pHairyRed: a novel binary vector containing the DsRed2 reporter gene
            for visual selection of transgenic hairy roots.
  JOURNAL   Mol Plant. 2011 May;4(3):537-45. doi: 10.1093/mp/ssq084. Epub 2011 
            Feb 15.
  PUBMED    21324970
REFERENCE   2  (bases 1 to 10741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/ssq084";
            journalName: "Mol Plant"; date: "2011-05"; volume: "4"; issue: "3"; 
            pages: "537-45"
COMMENT     Sequence Label: pHairyRed
FEATURES             Location/Qualifiers
     source          1..10741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    354..375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(390..395,396..413,414..420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    428..444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     433..455
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     452..468
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     452..468
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             469..696
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    477..533
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(537..554)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(537..553)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(546..568)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        930..1275
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          1307..1496
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1552..2229
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(1732..1752)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2144..2167
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     terminator      2265..2517
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2539..2563
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3864..4493
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4922..5995
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6061..6255
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6491..6513)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6599..6739
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6654..6673
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6754..6771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6925..7513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7005..7024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7600..8394)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8819..8843
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8921..9095
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9136..10161)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(join(10228..10741,1..163))
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
       61 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
      121 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
      181 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc
      241 aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      301 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      361 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      421 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat gattacgaat
      481 tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag cttggcactg
      541 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
      601 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
      661 tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt
      721 gtcgtttccc gccttcagtt tagcttcatg gagtcaaaga ttcaaataga ggacctaaca
      781 gaactcgccg taaagactgg cgaacagttc atacagagtc tcttacgact caatgacaag
      841 aagaaaatct tcgtcaacat ggtggagcac gacacacttg tctactccaa aaatatcaaa
      901 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
      961 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     1021 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     1081 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     1141 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     1201 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     1261 catttggaga gaacacgggg gactcttgac catggtagat ctgagggtaa atttctagtt
     1321 tttctccttc attttcttgg ttaggaccct tttctctttt tatttttttg agctttgatc
     1381 tttctttaaa ctgatctatt ttttaattga ttggttatgg tgtaaatatt acatagcttt
     1441 aactgataat ctgattactt tatttcgtgt gtctatgatg atgatgatag ttacagaacc
     1501 gacgaactag taacggccgc cagtgtgctg gaattcgccc ttcatgcaac tatggcctcc
     1561 tccgagaacg tcatcaccga gttcatgcgc ttcaaggtgc gcatggaggg caccgtgaac
     1621 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg ccacaacacc
     1681 gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
     1741 cagttccagt acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag
     1801 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
     1861 gtggcgaccg tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag
     1921 ttcatcggcg tgaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg
     1981 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gacccacaag
     2041 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc
     2101 aagaagcccg tgcagctgcc cggctactac tacgtggacg ccaagctgga catcacctcc
     2161 cacaacgagg actacaccat cgtggagcag tacgagcgca ccgagggccg ccaccacctg
     2221 ttcctgtagc cacgtgtgaa ttggtgacca gctcgaattt ccccgatcgt tcaaacattt
     2281 ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat
     2341 ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga
     2401 gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa
     2461 tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg
     2521 aattaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     2581 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     2641 atgtgcatgc caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg
     2701 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     2761 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     2821 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     2881 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     2941 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     3001 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     3061 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     3121 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     3181 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     3241 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     3301 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     3361 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     3421 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     3481 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     3541 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     3601 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     3661 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     3721 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     3781 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     3841 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     3901 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     3961 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     4021 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     4081 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     4141 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     4201 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     4261 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     4321 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     4381 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     4441 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     4501 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     4561 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     4621 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     4681 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     4741 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     4801 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     4861 gcggctgggt tgcctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     4921 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     4981 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     5041 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     5101 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     5161 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     5221 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     5281 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     5341 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     5401 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     5461 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     5521 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     5581 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     5641 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     5701 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     5761 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     5821 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     5881 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     5941 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     6001 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     6061 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     6121 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     6181 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     6241 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     6301 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     6361 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     6421 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
     6481 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6541 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
     6601 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     6661 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     6721 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6841 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     7381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7561 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
     7621 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
     7681 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
     7741 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
     7801 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
     7861 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
     7921 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
     7981 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
     8041 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
     8101 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     8161 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     8221 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     8281 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     8341 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     8401 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     8461 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     8521 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
     8581 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
     8641 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
     8701 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
     8761 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
     8821 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
     8881 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
     8941 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
     9001 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
     9061 tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc
     9121 cggtcggcat ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact
     9181 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
     9241 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
     9301 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
     9361 gagcatatac gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
     9421 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
     9481 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     9541 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     9601 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     9661 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     9721 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     9781 aagatcggcc gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag
     9841 ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt
     9901 caggctctcg ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     9961 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
    10021 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
    10081 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
    10141 ggtgagttca ggctttttca tatctcattg ccccccggat ctgcgaaagc tcgagagaga
    10201 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc
    10261 ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt
    10321 ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
    10381 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
    10441 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
    10501 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
    10561 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
    10621 ggagtagacg agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg
    10681 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
    10741 c
//
