LOCUS       sequence-324292-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive murine TrkB expression in mammalian host, controlled 
            under cytomegalovirus (CMV) promoter and enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet 
            ML, Mansy SS
  TITLE     Artificial cells drive neural differentiation.
  JOURNAL   Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 
            10.1126/sciadv.abb4920. Print 2020 Sep.
  PUBMED    32948587
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-09-18"; volume: "6(38). pii: 6/38/eabb4920. doi"; pages:
            " 10.1126/sciadv.abb4920. Print 2020 Sep"
COMMENT     Sequence Label: DT041A
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    360..593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             778..822
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1089..1205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1228..1531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1532..1735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1686..1706
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1732..1756
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4318..4906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4371..4391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4789..4800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4920..5419
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4955..4978)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5352..5371
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5440..6039
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5440..5459)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5936..5956
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6177..6410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6482..6603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6519..6538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6573..6592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6643..6778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6798..6817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6799..6817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6827..6844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6827..6843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6836..6858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6985..7440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7072..7091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7282..7303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7466..7570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7571..7639,7640..8431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7789..7808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8602..9190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9091..9110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9344..9361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9478..9499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9514..9519,9520..9537,9538..9544)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9552..9568
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9557..9579
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9576..9592
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9576..9592
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9611..9631
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9613..9631
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9659..9885
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9886..10066
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10113..10238
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
       61 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
      121 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
      181 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
      241 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
      301 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
      361 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
      421 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
      481 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
      541 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
      601 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
      661 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
      721 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
      781 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
      841 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
      901 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
      961 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     1021 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     1081 cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1141 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa
     1201 attttatcga taagcttggg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1261 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1321 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1381 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1441 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1501 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1561 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1621 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1681 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
     1741 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     1801 ccgactctag aggatccacc ggtcgccacc atgtcgccct ggctgaagtg gcatggaccc
     1861 gccatggcgc ggctctgggg cttatgcctg ctggtcttgg gcttctggag ggcctctctc
     1921 gcctgcccga cgtcctgcaa atgcagttcc gctaggattt ggtgtactga gccttctcca
     1981 ggcatcgtgg cattcccgag gttggaacct aacagcgttg acccggagaa catcacggaa
     2041 attctcattg caaaccagaa aaggctggaa atcatcaatg aagatgacgt tgaagcttac
     2101 gtggggctga gaaaccttac aattgtggat tccggcttaa agtttgtggc ttacaaagcg
     2161 tttctgaaaa acagcaacct gcggcacata aatttcacac gaaacaagct gacgagtttg
     2221 tccaggagac atttccgcca ccttgacttg tctgacctga tcctgacggg taatccgttc
     2281 acgtgctcct gcgacatcat gtggctcaag actctccagg agactaaatc cagccccgac
     2341 actcaggatt tgtactgcct caatgagagc agcaagaaca tgcccctggc gaacctgcag
     2401 atacccaatt gtggtctgcc atctgcacgt ctggctgctc ctaacctcac cgtggaggaa
     2461 ggaaagtctg tgaccctttc ctgcagtgtg gggggtgacc cactccccac cttgtactgg
     2521 gacgttggga atttggtttc caagcacatg aatgaaacaa gccacacaca gggctcctta
     2581 aggataacga acatttcatc tgatgacagt ggaaagcaaa tctcttgtgt ggcagaaaac
     2641 cttgtaggag aagatcaaga ttctgtgaac ctcactgtgc attttgcgcc aactatcacg
     2701 tttctcgagt ctccaacctc agatcaccac tggtgcattc cattcactgt gagaggcaac
     2761 cccaagcctg cgcttcagtg gttctacaat ggggccatac tgaatgagtc caagtacatc
     2821 tgtactaaga tccacgtcac caatcacacg gagtaccatg gctgcctcca gctggataac
     2881 cccactcata tgaataacgg agactacacc ctgatggcca agaacgagta tgggaaggat
     2941 gagagacaga tctccgctca cttcatgggc cggcctggag tcgactacga gacaaaccca
     3001 aattaccctg aagtcctcta tgaagactgg accacgccaa ctgacattgg ggatactacg
     3061 aacaaaagta atgaaatccc ctccacggat gttgctgacc aaagcaatcg ggagcatctc
     3121 tcggtctatg ccgtggtggt gattgcatct gtggtgggat tctgcctgct ggtgatgttg
     3181 ctcctgctca agttggcgag acattccaag tttggcatga aaggcccagc ttcggtcatc
     3241 agcaacgacg atgactctgc cagccccctc caccacatct ccaatgggag taacactcca
     3301 tcttcttcgg agggcggtcc cgacgctgtc attattggaa tgaccaagat tcctgttatt
     3361 gaaaaccccc agtactttgg catcaccaac agtcagctca agccagacac atttgttcag
     3421 catatcaaga gacacaacat cgttctgaag agggaacttg gggaaggagc cttcgggaaa
     3481 gttttccttg ccgagtgcta caacctctgc ccagagcagg ataagatcct ggtggctgtg
     3541 aagacgctga aggacgccag cgacaatgca cgcaaggact ttcatcggga agctgagctg
     3601 ctgaccaacc tccagcacga gcacattgtc aagttctacg gtgtctgtgt ggagggcgac
     3661 ccactcatca tggtctttga gtacatgaag cacggggacc tcaacaagtt ccttagggca
     3721 cacgggcccg acgcagtgct gatggcagag ggtaacccgc ccacagagct gacgcagtcg
     3781 cagatgctgc acatcgctca gcaaatcgca gcaggtatgg tctacctggc gtcccaacac
     3841 tttgtgcacc gtgacctggc cacccggaac tgcctggtgg gagagaacct gctggtgaaa
     3901 attggggact ttgggatgtc ccgagatgtg tacagcaccg actactatcg ggtcggtggc
     3961 cacacaatgt tgcccatccg atggatgcct ccagagagca tcatgtacag gaaattcacc
     4021 accgagagcg acgtctggag cctgggcgtt gtgttgtggg agatcttcac ctacggcaag
     4081 cagccctggt atcagctatc gaacaatgag gtgatagagt gcatcaccca gggaagagtc
     4141 cttcagcggc ctcgaacgtg tccccaggag gtgtatgagc tcatgcttgg atgctggcag
     4201 cgggaaccac acacccggaa gaacatcaag agcatccaca ccctccttca gaacttggcc
     4261 aaggcatctc ccgtctacct ggatatccta ggctagtagt aaagcggccg cgtcgacaat
     4321 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     4381 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     4441 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     4501 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     4561 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     4621 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     4681 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     4741 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4801 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4861 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattctaccg
     4921 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     4981 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     5041 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     5101 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     5161 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     5221 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     5281 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     5341 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     5401 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     5461 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     5521 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     5581 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     5641 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     5701 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     5761 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     5821 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     5881 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     5941 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     6001 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag
     6061 cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac ctttaagacc
     6121 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6181 agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     6241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6421 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6481 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6541 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6601 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     6661 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     6721 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     6781 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6841 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6901 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6961 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7021 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7081 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7141 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7201 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7261 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7321 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7381 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7441 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7501 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7561 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7621 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     7681 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     7741 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     7801 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     7861 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     7921 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     7981 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8041 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8101 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8161 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8221 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8281 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8341 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8401 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8461 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8521 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8581 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     8641 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     8701 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     8761 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     8821 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     8881 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     8941 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     9001 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     9061 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     9121 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     9181 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     9241 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     9301 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     9361 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     9421 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     9481 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     9541 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     9601 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     9661 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     9721 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     9781 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     9841 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     9901 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9961 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10021 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
    10081 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
    10141 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
    10201 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
    10261 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
    10321 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc c
//
