LOCUS       sequence-32430-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-QF
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(374..390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(375..391)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     protein_bind    581..650
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     protein_bind    695..728
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atcacacttt tcacgtgcag ctcgacactg atcacaggcc ctcgagactc tctggcgttt
       61 cgaacgagcg ggctcgacaa gtaacgtcgt cgtggtaggg ccgaggccgg gtgtgtcaat
      121 gttggtcaag tccgcaaaac gggcgttgtt gctgcttgct gcagtgttag cgacatgacc
      181 atcagcattg ccatcagcat ggccatcagc gtgggcattg gcttcggcag cggcattgag
      241 tgtcttgcgt ttaggcggca ttttggatcc acgtaagggt taatgttttc aaaaaaaaat
      301 tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta agtgtatact
      361 tcggtaagct tatcgatacc gtcgacctcg acatgcccgc cgtgaccgtc gagaacccgc
      421 tgacgctgcc ccgcgtatcc gcacccgccg acgccgtcgc acgtcccgtg ctcaccgtga
      481 ccaccgcgcc cagcggtttc gagggcgagg gcttcccggt gcgccgcgcg ttcgccggga
      541 tcaactaccg ccacctcgac ccgttcatca tgatggacca gatgggtgag gtggagtacg
      601 cgcccgggga gcccaagggc acgccctggc acccgcaccg cggcttcgag accgtgacct
      661 acatcgtcga gggggggccc ggtactggcg cgccgaagtt cctattctct agaaagtata
      721 ggaacttcgg taccgaattc ggctgctgct ctaaacgacg catttcgtac tccaaagtac
      781 gaattttttc cctcaagctc ttattttcat taaacaatga acaggacct
//
