LOCUS       sequence-32431-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-QF
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     161..179
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    364..397
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     434..450
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     434..450
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata
       61 cccttggatc gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta
      121 aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata
      181 catactagaa ttcgatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
      241 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
      301 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
      361 gctataactt cgtataatgt atgctatacg aagttatcta gcctaggcat gcttaattaa
      421 ctcgagcggc cgctctagaa ctagtggatc cgcggccgcg gatctaaacg agtttttaag
      481 caaactcact ccctgacaat aaaaacgcaa ttatctttaa ataaatatat tctatttatt
      541 aaccaagtag ctaatatagc taaaatcaat ttgttgctaa cttaaaacta acataattat
      601 gtattagtta tttacattct tttggttggt tacaaccaaa taaataatat cacttttaaa
      661 aacttaagcc aggaactgaa taattttcta tttggcttta gtcgacggta tcgataagat
      721 ctgcactagt gcttactatt gctcatacgt gttgatatcg ctaaaattac accctgggac
      781 aaacccgagg ttcgtcatga actgcggatc aacgtcaacc gcatccgtgt actcgatcga
      841 gc
//
