LOCUS       sequence-32432-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-QF
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(134..167)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(191..224)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    join(454..655,656..686)
                     /label=P element 3' end
                     /note="P element 3' end"
ORIGIN
        1 gttccttgtg tagatgcatc tcaaaaaaat ggtgggcata atagtgttgt ttatatatat
       61 caaaaataac aactataata ataagaatac atttaattta gaaaatgctt ggatttcact
      121 ggaactagaa ttcataactt cgtatagcat acattatacg aagttatgga tctggccgcg
      181 ctcttccgct gaagttccta tactttctag agaataggaa cttcctcgac ggtagcggtc
      241 cggttgtttt cgtgctcatc gcgagtacgt atttattttt aaaaaataat aaacgcggca
      301 aacacaatca cacaaatgtg caggcacagt gggacaaagt gcgctaaatt tggatatgta
      361 ataaatccaa aaccaaaaag aaaacaaaaa acggtaaaat attttactgt tgttaaaatt
      421 cgatcattca ttattcgctg catgaattag ctttgcgtac tcgcaaatta ttaaaaataa
      481 aactttaaaa ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg
      541 ctaagggtta atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat
      601 actattcctt tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg
      661 ggaccacctt atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct
      721 cgcatatctg gctctaagac ttcgggcccg acgcaggagt agccgacata tatccgaaat
      781 aactgct
//
