LOCUS       sequence-324333-        7273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of human BCL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Caeser R, Di Re M, Krupka JA, Gao J, Lara-Chica M, Dias JML, Cooke 
            SL, Fenner R, Usheva Z, Runge HFP, Beer PA, Eldaly H, Pak HK, Park 
            CS, Vassiliou GS, Huntly BJP, Mupo A, Bashford-Rogers RJM, Hodson DJ
  TITLE     Genetic modification of primary human B cells to model high-grade 
            lymphoma.
  JOURNAL   Nat Commun. 2019 Oct 4;10(1):4543. doi: 10.1038/s41467-019-12494-x.
  PUBMED    31586074
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-4"; volume: "10(1):4543. doi"; pages: " 
            10.1038/s41467-019-12494-x"
COMMENT     Sequence Label: MSCV-BCL2
FEATURES             Location/Qualifiers
     source          1..7273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             84..600
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    664..1005
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1072..1488
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1418..1440
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1456..1472
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    2273..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2290..2842
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             3751..4265
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    4427..4443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4451..4457,4458..4475,4476..4481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4496..4517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4634..4651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4805..5393)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4885..4904)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5564..6355,6356..6424))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6187..6206
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6425..6529)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6597..6615
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6653..6675)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6775..6794
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6988..7010
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7138..7157
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 caacaatatc accagctgaa gcctatagag tacgagccat agataaaata aaagatttta
       61 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
      121 taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat
      181 caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag
      241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt
      481 cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc
      541 gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt
      601 tggaggttcc accgagattt ggagacccct gcccagggac caccgacccc cccgccggga
      661 ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc
      721 atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc
      781 cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac
      841 tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg
      901 tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa
      961 tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc
     1021 gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact
     1081 gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac
     1141 aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca
     1201 acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt
     1261 aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg
     1321 acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag
     1381 cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc
     1441 ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg aattagatct
     1501 ctcgaggtta accaccatgg cgcacgctgg gagaacaggg tacgataacc gggagatagt
     1561 gatgaagtac atccattata agctgtcgca gaggggctac gagtgggatg cgggagatgt
     1621 gggagccgct cctcctggtg ccgctcccgc accgggcatc ttctcctccc agcctgggca
     1681 cacgcctcat ccagccgcat cccgggatcc tgtcgccagg acctcgcctc tgcagacacc
     1741 ggctgctcct ggagctgctg caggacctgc gctcagcccg gtgccacctg tggtccacct
     1801 gaccctccgc caggccggcg acgacttctc ccgccgctac cgccgcgact tcgccgagat
     1861 gtccagccag ctgcacctga cgcccttcac cgcgcgggga cgctttgcca cggtggtgga
     1921 ggagctcttc agggacgggg tgaactgggg gaggattgtg gccttctttg agttcggtgg
     1981 ggtcatgtgt gtggagagcg tcaaccggga gatgtcgccc ctggtggaca acatcgccct
     2041 gtggatgact gagtacctga accggcacct gcacacctgg atccaggata acggaggctg
     2101 ggatgccttt gtggaactgt acggccccag catgcggcct ctgtttgatt tctcctggct
     2161 gtctctgaag actctgctca gtttggccct ggtgggagct tgcatcaccc tgggtgccta
     2221 tctgggccac aagtgataga acgcggccgc accggtggat cctacgtaga attccgcccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa tatggccaca accatggtga gctttccatg taaatttgta
     2881 gccagcttcc ttctgatttt caatgtttct tccaaaggtg cagtctccaa agagattacg
     2941 aatgccttgg aaacctgggg tgccttgggt caggacatca acttggacat tcctagtttt
     3001 caaatgagtg atgatattga cgatataaaa tgggaaaaaa cttcagacaa gaaaaagatt
     3061 gcacaattca gaaaagagaa agagactttc aaggaaaaag atacatataa gctatttaaa
     3121 aatggaactc tgaaaattaa gcatctgaag accgatgatc aggatatcta caaggtatca
     3181 atatatgata caaaaggaaa aaatgtgttg gaaaaaatat ttgatttgaa gattcaagag
     3241 agggtctcaa aaccaaagat ctcctggact tgtatcaaca caaccctgac ctgtgaggta
     3301 atgaatggaa ctgaccccga attaaacctg tatcaagatg ggaaacatct aaaactttct
     3361 cagagggtca tcacacacaa gtggaccacc agcctgagtg caaaattcaa gtgcacagca
     3421 gggaacaaag tcagcaagga atccagtgtc gagcctgtca gctgtccaga gaaaggtctg
     3481 gacatctatc tcatcattgg catatgtgga ggaggcagcc tcttgatggt ctttgtggca
     3541 ctgctcgttt tctatatcac caaaaggaaa aaacagagga gtcggagaaa tgatgaggag
     3601 ctggagacaa gagcccacag agtagctact gaagaaaggg gccggaagcc ccaccaaatt
     3661 ccagcttcaa cccctcagaa ttaagtcgac ctgcagccaa gcttatcgat aaaataaaag
     3721 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     3781 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     3841 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     3901 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     3961 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     4021 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     4081 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     4141 ctgcgtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
     4201 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
     4261 ttcatgggta acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat
     4321 taagtaatcc tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa
     4381 tagttcatta tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca
     4441 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4501 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4561 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4621 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4681 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4741 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4801 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4861 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4921 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4981 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     5041 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5101 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5161 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5221 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5281 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5341 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5401 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5461 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5521 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5581 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5641 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5701 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5761 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5821 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5881 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5941 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6001 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6061 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6121 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6181 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6241 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6301 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6361 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6421 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6481 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6541 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6601 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6661 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6721 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     6781 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     6841 ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
     6901 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     6961 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggcgcaag
     7021 gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat
     7081 acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt
     7141 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat
     7201 gcgtccggcg tagaggcgat tagtccaatt tgttaaagac aggatatcag tggtccaggc
     7261 tctagttttg act
//
