LOCUS       sequence-324338-       12546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   McKenzie GJ, Craig NL
  TITLE     Fast, easy and efficient: site-specific insertion of transgenes into
            enterobacterial chromosomes using Tn7 without need for selection of 
            the insertion event.
  JOURNAL   BMC Microbiol. 2006 Apr 28;6:39. doi: 10.1186/1471-2180-6-39.
  PUBMED    16646962
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2006-04-28"; volume: "6:39. doi"; pages: " 
            10.1186/1471-2180-6-39"
COMMENT     Sequence Label: pGRG25
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(176..1054)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1081..1365
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1288..1307
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(8056..8073)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8226..8312
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8404..8431
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        8450..8541
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8542..8610,8611..9402)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRXDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8760..8779)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(9531..9550)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     oriT            9573..9682
                     /note="incP origin of transfer"
     primer_bind     complement(9720..9739)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     rep_origin      10422..10644
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             10692..11642
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     mobile_element  12075..12240
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttcagtttaa gactttattg tccgcccaca ggccacgatg cgtccggcgt agaggatctg
       61 ctcatgtttg acagcttatc atcgatgcat aatgtgcctg tcaaatggac gaagcaggga
      121 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
      181 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
      241 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
      301 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
      361 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
      421 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
      481 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
      541 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
      601 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
      661 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg
      721 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
      781 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
      841 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
      901 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
      961 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
     1021 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
     1081 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
     1141 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
     1201 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
     1261 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
     1321 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
     1381 ctagcgaatt cgagctcggt acccaagtct taaactagac agaatagttg taaactgaaa
     1441 tcagtccagt tatgctgtga aaaagcatac tggacttttg ttatggctaa agcaaactct
     1501 tcattttctg aagtgcaaat tgcccgtcgt attaaagagg ggcgtggcca agggcatggt
     1561 aaagactata ttccatggct aacagtacaa gaagttcctt cttcaggtcg ttcccaccgt
     1621 atttattctc ataagacggg acgagtccat catttgctat ctgacttaga gcttgctgtt
     1681 tttctcagtc ttgagtggga gagcagcgtg ctagatatac gcgagcagtt ccccttatta
     1741 cctagtgata ccaggcagat tgcaatagat agtggtatta agcatcctgt tattcgtggt
     1801 gtagatcagg ttatgtctac tgatttttta gtggactgca aagatggtcc ttttgagcag
     1861 tttgctattc aagtcaaacc tgcagcagcc ttacaagacg agcgtacctt agaaaaacta
     1921 gaactagagc gtcgctattg gcagcaaaag caaattcctt ggttcatttt tactgataaa
     1981 gaaataaatc ccgtagtaaa agaaaatatt gaatggcttt attcagtgaa aacagaagaa
     2041 gtttctgcgg agcttttagc acaactatcc ccattggccc atatcctgca agaaaaagga
     2101 gatgaaaaca ttatcaatgt ctgtaagcag gttgatattg cttatgattt ggagttaggc
     2161 aaaacattga gtgagatacg agccttaacc gcaaatggtt ttattaagtt caatatttat
     2221 aagtctttca gggcaaataa gtgtgcagat ctctgtatta gccaagtagt gaatatggag
     2281 gagttgcgct atgtggcaaa ttaatgaggt tgtgctattt gataatgatc cgtatcgcat
     2341 tttggctata gaggatggcc aagttgtctg gatgcaaata agcgctgata aaggagttcc
     2401 acaagctagg gctgagttgt tgctaatgca gtatttagat gaaggccgct tagttagaac
     2461 tgatgaccct tatgtacatc ttgatttaga agagccgtct gtagattctg tcagcttcca
     2521 gaagcgcgag gaggattatc gaaaaattct tcctattatt aatagtaagg atcgtttcga
     2581 ccctaaagtc agaagcgaac tcgttgagca tgtggtccaa gaacataagg ttactaaggc
     2641 tacagtttat aagttgttac gccgttactg gcagcgtggt caaacgccta atgcattaat
     2701 tcctgactac aaaaacagcg gtgcaccagg ggaaagacgt tcagcgacag gaacagcaaa
     2761 gattggccga gccagagaat atggtaaggg tgaaggaacc aaggtaacgc ccgagattga
     2821 acgccttttt aggttgacca tagaaaagca cctgttaaat caaaaaggta caaagaccac
     2881 cgttgcctat agacgatttg tggacttgtt tgctcagtat tttcctcgca ttccccaaga
     2941 ggattaccca acactacgtc agtttcgtta tttttatgat cgagaatacc ctaaagctca
     3001 gcgcttaaag tctagagtta aagcaggggt atataaaaaa gacgtacgac ccttaagtag
     3061 tacagccact tctcaggcgt taggccctgg gagtcgttat gagattgatg ccacgattgc
     3121 tgatatttat ttagtggatc atcatgatcg ccaaaaaatc ataggaagac caacgcttta
     3181 cattgtgatt gatgtgttta gtcggatgat cacgggcttt tatatcggct ttgaaaatcc
     3241 gtcttatgtg gtggcgatgc aggcttttgt aaatgcttgc tctgacaaaa cggccatttg
     3301 tgcccagcat gatattgaga ttagtagctc agactggccg tgtgtaggtt tgccagatgt
     3361 gttgctagcg gaccgtggcg aattaatgag tcatcaggtc gaagccttag tttctagttt
     3421 taatgtgcga gtggaaagtg ctccacctag acgtggcgat gctaaaggca tagtggaaag
     3481 cacttttaga acactacaag ccgagtttaa gtcctttgca cctggcattg tagagggcag
     3541 tcggatcaaa agccatggtg aaacagacta taggttagat gcatctctgt cggtatttga
     3601 gttcacacaa attattttgc gtacgatctt attcagaaat aaccatctgg tgatggataa
     3661 atacgatcga gatgctgatt ttcctacaga tttaccgtct attcctgtcc agctatggca
     3721 atggggtatg cagcatcgta caggtagttt aagggctgtg gagcaagagc agttgcgagt
     3781 agcgttactg cctcgccgaa aggtctctat ttcttcattt ggcgttaatt tgtggggttt
     3841 gtattactcg gggtcagaga ttctgcgtga gggttggttg cagcggagca ctgatatagc
     3901 tagacctcaa catttagaag cggcttatga cccagtgctg gttgatacga tttatttgtt
     3961 tccgcaagtt ggcagccgtg tattttggcg ctgtaatctg acggaacgta gtcggcagtt
     4021 taaaggtctc tcattttggg aggtttggga tatacaagca caagaaaaac acaataaagc
     4081 caatgcgaag caggatgagt taactaaacg cagggagctt gaggcgttta ttcagcaaac
     4141 cattcagaaa gcgaataagt taacgcccag tactactgag cccaaatcaa cacgcattaa
     4201 gcagattaaa actaataaaa aagaagccgt gacctcggag cgtaaaaaac gtgcggagca
     4261 tttgaagcca agctcttcag gtgatgaggc taaagttatt cctttcaacg cagtggaagc
     4321 ggatgatcaa gaagattaca gcctacccac atacgtgcct gaattatttc aggatccacc
     4381 agaaaaggat gagtcatgag tgctacccgg attcaagcag tttatcgtga tacgggggta
     4441 gaggcttatc gtgataatcc ttttatcgag gccttaccac cattacaaga gtcagtgaat
     4501 agtgctgcat cactgaaatc ctctttacag cttacttcct ctgacttgca aaagtcccgt
     4561 gttatcagag ctcataccat ttgtcgtatt ccagatgact attttcagcc attaggtacg
     4621 catttgctac taagtgagcg tatttcggtc atgattcgag gtggctacgt aggcagaaat
     4681 cctaaaacag gagatttaca aaagcattta caaaatggtt atgagcgtgt tcaaacggga
     4741 gagttggaga catttcgctt tgaggaggca cgatctacgg cacaaagctt attgttaatt
     4801 ggttgttctg gtagtgggaa gacgacctct cttcatcgta ttctagccac gtatcctcag
     4861 gtgatttacc atcgtgaact caatgtagag caggtggtgt atttgaaaat agactgctcg
     4921 cataatggtt cgctaaaaga aatctgcttg aattttttca gagcgttgga tcgagccttg
     4981 ggctcgaact atgagcgtcg ttatggctta aaacgtcatg gtatagaaac catgttggct
     5041 ttgatgtcgc aaatagccaa tgcacatgct ttagggttgt tggttattga tgaaattcag
     5101 catttaagcc gctctcgttc gggtggatct caagagatgc tgaacttttt tgtgacgatg
     5161 gtgaatatta ttggcgtacc agtgatgttg attggtaccc ctaaagcacg agagattttt
     5221 gaggctgatt tgcggtctgc acgtagaggg gcagggtttg gagctatatt ctgggatcct
     5281 atacaacaaa cgcaacgtgg aaagcccaat caagagtgga tcgcttttac ggataatctt
     5341 tggcaattac agcttttaca acgcaaagat gcgctgttat cggatgaggt ccgtgatgtg
     5401 tggtatgagc taagccaagg agtgatggac attgtagtaa aactttttgt actcgctcag
     5461 ctccgtgcgc tagctttagg caatgagcgt attaccgctg gtttattgcg gcaagtgtat
     5521 caagatgagt taaagcctgt gcaccccatg ctagaggcat tacgctcggg tatcccagaa
     5581 cgcattgctc gttattctga tctagtcgtt cccgagattg ataaacggtt aatccaactt
     5641 cagctagata tcgcagcgat acaagaacaa acaccagaag aaaaagccct tcaagagtta
     5701 gataccgaag atcagcgtca tttatatctg atgctgaaag aggattacga ttcaagcctg
     5761 ttaattccca ctattaaaaa agcgtttagc cagaatccaa cgatgacaag acaaaagtta
     5821 ctgcctcttg ttttgcagtg gttgatggaa ggcgaaacgg tagtgtcaga actagaaaag
     5881 ccctccaaga gtaaaaaggt ttcggctata aaggtagtca agcccagcga ctgggatagc
     5941 ttgcctgata cggatttacg ttatatctat tcacaacgcc aacctgaaaa aaccatgcat
     6001 gaacggttaa aagggaaagg ggtaatagtg gatatggcga gcttatttaa acaagcaggt
     6061 tagccatgag aaactttcct gttccgtact cgaatgagct gatttatagc actattgcac
     6121 gggcaggcgt ttatcaaggg attgttagtc ctaagcagct gttggatgag gtgtatggca
     6181 accgcaaggt ggtcgctacc ttaggtctgc cctcgcattt aggtgtgata gcaagacatc
     6241 tacatcaaac aggacgttac gctgttcagc agcttattta tgagcatacc ttattccctt
     6301 tatatgctcc gtttgtaggc aaggagcgcc gagacgaagc tattcggtta atggagtacc
     6361 aagcgcaagg tgcggtgcat ttaatgctag gagtcgctgc ttctagagtt aagagcgata
     6421 accgctttag atactgccct gattgcgttg ctcttcagct aaataggtat ggggaagcct
     6481 tttggcaacg agattggtat ttgcccgctt tgccatattg tccaaaacac ggtgctttag
     6541 tcttctttga tagagctgta gatgatcacc gacatcaatt ttgggctttg ggtcatactg
     6601 agctgctttc agactacccc aaagactccc tatctcaatt aacagcacta gctgcttata
     6661 tagcccctct gttagatgct ccacgagcgc aagagctttc cccaagcctt gagcagtgga
     6721 cgctgtttta tcagcgctta gcgcaggatc tagggctaac caaaagcaag cacattcgtc
     6781 atgacttggt ggcggagaga gtgaggcaga cttttagtga tgaggcacta gagaaactgg
     6841 atttaaagtt ggcagagaac aaggacacgt gttggctgaa aagtatattc cgtaagcata
     6901 gaaaagcctt tagttattta cagcatagta ttgtgtggca agccttattg ccaaaactaa
     6961 cggttataga agcgctacag caggcaagtg ctcttactga gcactctata acgacaagac
     7021 ctgttagcca gtctgtgcaa cctaactctg aagatttatc tgttaagcat aaagactggc
     7081 agcaactagt gcataaatac caaggaatta aggcggcaag acagtcttta gagggtgggg
     7141 tgctatacgc ttggctttac cgacatgaca gggattggct agttcactgg aatcaacagc
     7201 atcaacaaga gcgtctggca cccgccccta gagttgattg gaaccaaaga gatcgaattg
     7261 ctgtacgaca actattaaga atcataaagc gtctagatag tagccttgat cacccaagag
     7321 cgacatcgag ctggctgtta aagcaaactc ctaacggaac ctctcttgca aaaaatctac
     7381 agaaactgcc tttggtagcg ctttgcttaa agcgttactc agagagtgtg gaagattatc
     7441 aaattagacg gattagccaa gcttttatta agcttaaaca ggaagatgtt gagcttaggc
     7501 gctggcgatt attaagaagt gcaacgttat ctaaagagcg gataactgag gaagcacaaa
     7561 gattcttgga aatggtttat ggggaagagt gagtggttag gctagctaca tttaatgaca
     7621 atgtgcaggt tgtacatatt ggtcatttat tccgtaactc gggtcataag gagtggcgta
     7681 tttttgtttg gtttaatcca atgcaagaac ggaaatggac tcgatttact catttgcctt
     7741 tattaagtcg agctaaggtg gttaacagta caacaaagca aataaataag gcggatcgtg
     7801 tgattgagtt tgaagcatcg gatcttcaac gagccaaaat aatcgatttt cctaatctct
     7861 cgtcctttgc ttccgtacgc aacaaggatg gagcgcagag ttcatttatt tacgaagctg
     7921 aaacaccata tagcaagact cgttatcaca tcccacagtt agagctagct cggtcattat
     7981 ttttagggga tcctctagag tcgacctgca ggcatgcaag cttggctgtt ttggcggatg
     8041 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     8101 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     8161 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     8221 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     8281 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     8341 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     8401 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     8461 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8521 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     8581 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     8641 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     8701 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     8761 ctatgtggcg cggtattatc ccgtrttgac gccgggcaag agcaactcgg tcgccgcata
     8821 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     8881 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     8941 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9001 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9061 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     9121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9421 tcatatatac tttagattga tttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9481 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9541 tttcttccct tcctttctcg ccacgttcgc cgccggccag cctcgcagag caggattccc
     9601 gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg
     9661 gcctacttca cctatcctgc ccggcggcat caccggcgcc acaggtgcgg ttgctggcgc
     9721 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     9781 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     9841 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     9901 cttcctaatg caggagtcgc ataagggaga gcgtcgatcc ccgacagtaa gacgggtaag
     9961 cctgttgatg ataccgctgc cttactgggt gcattagcca gtctgaatga cctgtcacgg
    10021 gataatccga agtggtcaga ctggaaaatc agagggcagg aactgctgaa cagcaaaaag
    10081 tcagatagca ccacatagca gacccgccat aaaacgccct gagaagcccg tgacgggctt
    10141 ttcttgtatt atgggtagtt tccttgcatg aatccataaa aggcgcctgt agtgccattt
    10201 acccccattc actgccagag ccgtgagcgc agcgaactga atgtcacgaa aaagacagcg
    10261 actcaggtgc ctgatggtcg gagacaaaag gaatattcag cgatttgccc gagcttgcga
    10321 gggtgctact taagccttta gggttttaag gtctgttttg tagaggagca aacagcgttt
    10381 gcgacatcct tttgtaatac tgcggaactg actaaagtag tgagttatac acagggctgg
    10441 gatctattct ttttatcttt ttttattctt tctttattct ataaattata accacttgaa
    10501 tataaacaaa aaaaacacac aaaggtctag cggaatttac agagggtcta gcagaattta
    10561 caagttttcc agcaaaggtc tagcagaatt tacagatacc cacaactcaa aggaaaagga
    10621 ctagtaatta tcattgacta gcccatctca attggtatag tgattaaaat cacctagacc
    10681 aattgagatg tatgtctgaa ttagttgttt tcaaagcaaa tgaactagcg attagtcgct
    10741 atgacttaac ggagcatgaa accaagctaa ttttatgctg tgtggcacta ctcaacccca
    10801 cgattgaaaa ccctacaagg aaagaacgga cggtatcgtt cacttataac caatacgttc
    10861 agatgatgaa catcagtagg gaaaatgctt atggtgtatt agctaaagca accagagagc
    10921 tgatgacgag aactgtggaa atcaggaatc ctttggttaa aggctttgag attttccagt
    10981 ggacaaacta tgccaagttc tcaagcgaaa aattagaatt agtttttagt gaagagatat
    11041 tgccttatct tttccagtta aaaaaattca taaaatataa tctggaacat gttaagtctt
    11101 ttgaaaacaa atactctatg aggatttatg agtggttatt aaaagaacta acacaaaaga
    11161 aaactcacaa ggcaaatata gagattagcc ttgatgaatt taagttcatg ttaatgcttg
    11221 aaaataacta ccatgagttt aaaaggctta accaatgggt tttgaaacca ataagtaaag
    11281 atttaaacac ttacagcaat atgaaattgg tggttgataa gcgaggccgc ccgactgata
    11341 cgttgatttt ccaagttgaa ctagatagac aaatggatct cgtaaccgaa cttgagaaca
    11401 accagataaa aatgaatggt gacaaaatac caacaaccat tacatcagat tcctacctac
    11461 ataacggact aagaaaaaca ctacacgatg ctttaactgc aaaaattcag ctcaccagtt
    11521 ttgaggcaaa atttttgagt gacatgcaaa gtaagtatga tctcaatggt tcgttctcat
    11581 ggctcacgca aaaacaacga accacactag agaacatact ggctaaatac ggaaggatct
    11641 gaggttctta tggctcttgt atctatcagt gaagcatcaa gactaacaaa caaaagtaga
    11701 acaactgttc accgttacat atcaaaggga aaactgtcca tatgcacaga tgaaaacggt
    11761 gtaaaaaaga tagatacatc agagctttta cgagtttttg gtgcatttaa agctgttcac
    11821 catgaacaga tcgacaatgt aacagatgaa cagcatgtaa cacctaatag aacaggtgaa
    11881 accagtaaaa caaagcaact agaacatgaa attgaacacc tgagacaact tgttacagct
    11941 caacagtcac acatagacag cctgaaacag gcgatgctgc ttatcgaatc aaagctgccg
    12001 acaacacggg agccagtgac gcctcccgtg gggaaaaaat catggcaatt ctggaagaaa
    12061 tagcgctttc agcctgtggg cggacaaaat agttgggaac tgggaggggt ggaaatggag
    12121 tttttaagga ttatttaggg aagagtgaca aaatagatgg gaactgggtg tagcgtcgta
    12181 agctaatacg aaaattaaaa atgacaaaat agtttggaac tagatttcac ttatctggtt
    12241 ggtcgacact agtattaccc tgttatccct agatttaaat gatatcggat cctagtaagc
    12301 cacgttttaa ttaatcagat ccctcaatag cggccgccac tcgagcacct aggaggcgcg
    12361 ccacggccgc gtcgacccca cgcccctctt taatacgacg ggcaatttgc acttcagaaa
    12421 atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag cataactgga
    12481 ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata gtttagatct
    12541 attttg
//
