LOCUS       sequence-32434-e         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pXN-FBLWLF-VP16AD
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     169..187
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    372..405
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagcgctgac tttgagtgga atgtcatttt gagtgagagg taatcgaaag aacctggtac
       61 atcaaatacc cttggatcga agtaaattta aaactgatca gataagttca atgatatcca
      121 gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc gcaaaaatgg gttttattaa
      181 cttacataca tactagaatt cgatctctcg tgggatcatt gtttttctct tgattcccac
      241 tttgtggttc taagtactgt ggtttccaaa tgtgtcagtt tcatagcctg aagaacgaga
      301 tcagcagcct ctgttccaca tacacttcat tctcagtatt gttttgccaa gttctaattc
      361 catcagaagc tataacttcg tataatgtat gctatacgaa gttatctagc ctaggcatgc
      421 ttaattaagc tagtggatct aaacgagttt ttaagcaaac tcactccctg acaataaaaa
      481 cgcaattatc tttaaataaa tatattctat ttattaacca agtagctaat atagctaaaa
      541 tcaatttgtt gctaacttaa aactaacata attatgtatt agttatttac attcttttgg
      601 ttggttacaa ccaaataaat aatatcactt ttaaaaactt aagccaggaa ctgaataatt
      661 ttctatttgg ctttagtcga cggtatcgat ctagaggcgc gccttacttg ccgccgccca
      721 gggggccgta gcgggtgcgg ttctggctca cgcggttgcg caggcgctgc acgcgctggc
      781 gcagctcggc cacgcgggtc tccagggcgg tgttctcgcg ctccaggaag gcggcctcga
      841 tctccaga
//
