LOCUS       sequence-324361-        9098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9098)
  AUTHORS   Shen H, Forgacs D, Chapla D, Moremen KW, Wells L, Hamer SA, Tompkins
            SM, Ross TM, Rouphael N, Edupuganti S, Collins MH, Tarleton RL
  TITLE     A flexible, pan-species, multi-antigen platform for the detection 
            and monitoring of SARS-CoV-2-specific antibody responses.
  JOURNAL   medRxiv [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279.
  PUBMED    33532799
REFERENCE   2  (bases 1 to 9098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2021 Jan 27:2021.01.20.21249279. doi: 
            10.1101/2021.01.20.21249279."
COMMENT     Sequence Label: pET30a-Spike-E
FEATURES             Location/Qualifiers
     source          1..9098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     563..582
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(602..679)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     992..1011
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        992..1010
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1011..1035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1050..1072
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1083..1100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1110..1127
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1134..1178
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             4914..4937
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4950..4967
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5020..5038)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5034..5081
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5118..5573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5205..5224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5415..5436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5665..6480)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5665..6480)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6388..6407
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6472..6491
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6602..7190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7091..7110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7344..7361
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7376..7515
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7441..7460)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7601..7623
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7617..7808)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(8617..9098,1..601))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
       61 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
      121 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
      181 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
      241 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
      301 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
      361 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
      421 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
      481 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
      541 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
      601 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
      661 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      721 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      781 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      841 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga
     1021 gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga gatatacata
     1081 tgcaccatca tcatcatcat tcttctggtc tggtgccacg cggttctggt atgaaagaaa
     1141 ccgctgctgc taaattcgaa cgccagcaca tggacagccc agatctgggt accatgttcg
     1201 ttttcctggt tctgctgccg ctggttagca gccaatgcgt gaatctgacc acccgcaccc
     1261 aactgccgcc ggcgtacacc aacagcttca cccgtggtgt ttactatccg gacaaagttt
     1321 ttcgtagcag cgtgctgcac agcacccagg acctgttcct gccgttcttt agcaacgtta
     1381 cctggttcca cgcgatccac gtgagcggca ccaacggcac caagcgtttc gacaacccgg
     1441 tgctgccgtt taacgatggt gtttacttcg cgagcaccga gaagagcaac atcattcgtg
     1501 gttggatttt tggcaccacc ctggacagca aaacccagag cctgctgatc gttaacaacg
     1561 cgaccaacgt ggttattaag gtgtgcgaat tccaattctg caacgatccg ttcctgggcg
     1621 tttactatca caagaacaac aaaagctgga tggagagcga atttcgtgtt tatagcagcg
     1681 cgaacaactg cacctttgag tacgtgagcc agccgttcct gatggacctg gaaggcaagc
     1741 aaggcaactt caaaaacctg cgtgagttcg tgttcaagaa catcgatggt tacttcaaga
     1801 tctacagcaa gcacaccccg atcaacctgg ttcgtgacct gccgcagggt tttagcgcgc
     1861 tggagccgct ggttgacctg ccgatcggca ttaacatcac ccgttttcaa accctgctgg
     1921 cgctgcaccg tagctacctg accccgggtg acagcagcag cggttggacc gcgggtgcgg
     1981 cggcgtacta tgttggttat ctgcagccgc gtaccttcct gctgaaatac aacgaaaacg
     2041 gcaccatcac cgacgcggtt gattgcgcgc tggatccgct gagcgaaacc aagtgcaccc
     2101 tgaaaagctt taccgtggag aagggtattt atcagaccag caacttccgt gtgcaaccga
     2161 ccgaaagcat tgttcgtttt ccgaacatca ccaacctgtg cccgtttggc gaggttttca
     2221 acgcgacccg tttcgcgagc gtgtacgcgt ggaaccgtaa acgtatcagc aactgcgttg
     2281 cggactatag cgtgctgtac aacagcgcga gcttcagcac ctttaagtgc tatggtgtga
     2341 gcccgaccaa actgaacgat ctgtgcttta ccaacgttta cgcggatagc ttcgtgattc
     2401 gtggcgacga ggttcgtcag atcgcgccgg gtcaaaccgg caagattgcg gactacaact
     2461 ataaactgcc ggacgatttc accggttgcg ttatcgcgtg gaacagcaac aacctggata
     2521 gcaaagtggg tggcaactac aactatctgt accgtctgtt tcgtaagagc aacctgaaac
     2581 cgttcgagcg tgacattagc accgaaatct accaggcggg tagcaccccg tgcaacggtg
     2641 ttgagggctt taactgctat ttcccgctgc agagctacgg cttccaaccg accaacggtg
     2701 tgggctatca accgtaccgt gtggttgtgc tgagctttga actgctgcat gcgccggcga
     2761 ccgtttgcgg tccgaagaaa agcaccaacc tggttaagaa caaatgcgtg aacttcaact
     2821 ttaacggcct gaccggcacc ggtgtgctga ccgagagcaa caagaaattc ctgccgtttc
     2881 agcaattcgg tcgtgacatt gcggatacca ccgatgcggt gcgtgacccg cagaccctgg
     2941 aaattctgga tatcaccccg tgcagcttcg gtggcgttag cgtgatcacc ccgggcacca
     3001 acaccagcaa ccaggttgcg gtgctgtatc aagacgttaa ctgcaccgag gttccggtgg
     3061 cgattcacgc ggatcagctg accccgacct ggcgtgtgta cagcaccggt agcaacgttt
     3121 tccaaacccg tgcgggttgc ctgattggtg cggagcatgt gaacaacagc tatgaatgcg
     3181 acattccgat cggtgcgggc atttgcgcga gctaccagac ccaaaccaac agcccgggta
     3241 gcgcgagcag cgttgcgagc cagagcatca ttgcgtatac catgagcctg ggcgcggaaa
     3301 acagcgtggc gtacagcaac aacagcattg cgatcccgac caacttcacc attagcgtga
     3361 ccaccgagat cctgccggtt agcatgacca aaaccagcgt ggactgcacc atgtatatct
     3421 gcggtgatag caccgaatgc agcaacctgc tgctgcagta cggtagcttt tgcacccaac
     3481 tgaaccgtgc gctgaccggc attgcggtgg agcaggataa aaacacccaa gaagttttcg
     3541 cgcaggtgaa gcaaatttac aaaaccccgc cgatcaagga ctttggtggc ttcaacttta
     3601 gccagatcct gccggatccg agcaagccga gcaaacgtag ctttattgag gacctgctgt
     3661 tcaacaaggt taccctggcg gatgcgggtt tcatcaaaca gtatggtgat tgcctgggcg
     3721 acattgcggc gcgtgacctg atctgcgcgc aaaagtttaa cggcctgacc gtgctgccgc
     3781 cgctgctgac cgatgaaatg attgcgcagt acaccagcgc gctgctggcg ggcaccatta
     3841 ccagcggttg gacctttggt gcgggtgcgg cgctgcagat cccgtttgcg atgcaaatgg
     3901 cgtatcgttt caacggtatt ggcgttaccc aaaacgtgct gtacgagaac cagaagctga
     3961 tcgcgaacca atttaacagc gcgattggta aaatccagga tagcctgagc agcaccgcga
     4021 gcgcgctggg taaactgcaa gatgttgtga accagaacgc gcaagcgctg aacaccctgg
     4081 ttaagcagct gagcagcaac ttcggtgcga ttagcagcgt gctgaacgat attctgagcc
     4141 gtctggaccc gccggaggcg gaagttcaaa ttgaccgtct gatcaccggc cgtctgcaga
     4201 gcctgcaaac ctatgtgacc cagcaactga ttcgtgcggc ggaaattcgt gcgagcgcga
     4261 acctggcggc gaccaaaatg agcgagtgcg ttctgggtca gagcaagcgt gtggactttt
     4321 gcggtaaagg ctatcacctg atgagcttcc cgcagagcgc gccgcacggt gttgtgtttc
     4381 tgcacgttac ctacgtgccg gcgcaagaaa agaactttac caccgcgccg gcgatctgcc
     4441 atgatggtaa agcgcacttt ccgcgtgaag gtgttttcgt gagcaacggc acccactggt
     4501 ttgttaccca gcgtaacttc tacgagccgc aaatcattac caccgacaac accttcgtga
     4561 gcggtaactg cgatgttgtg attggcatcg ttaacaacac cgtgtatgat ccgctgcagc
     4621 cggagctgga cagctttaaa gaggagctgg ataagtactt caaaaaccac accagcccgg
     4681 acgttgatct gggtgacatt agcggcatca acgcgagcgt tgtgaacatt caaaaggaga
     4741 tcgaccgtct gaacgaagtg gcgaaaaacc tgaacgaaag cctgatcgat ctgcaggagc
     4801 tgggtaaata tgaacaaggt agcggctaca ttccggaggc gccgcgtgac ggtcaggcgt
     4861 atgtgcgtaa ggatggcgag tgggttctgc tgagcacctt tctgggtcgt agcctggaag
     4921 ttctgtttca aggtccgggt taactcgagc accaccacca ccaccactga gatccggctg
     4981 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat
     5041 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     5101 ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5161 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5221 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5281 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5341 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5401 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5461 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5521 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     5581 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5641 atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat gaaactgcaa
     5701 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     5761 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     5821 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     5881 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc
     5941 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     6001 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     6061 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     6121 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     6181 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     6241 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     6301 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     6361 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     6421 atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa tatggctcat
     6481 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg accaaaatcc
     6541 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6601 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6661 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6721 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     6781 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6841 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6901 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6961 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7021 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7081 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7141 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7201 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7261 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7321 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     7381 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt gcactctcag
     7441 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     7501 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     7561 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     7621 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     7681 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     7741 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     7801 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     7861 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     7921 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     7981 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     8041 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     8101 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     8161 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     8221 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggggcc
     8281 gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg
     8341 aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc
     8401 gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct
     8461 acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc
     8521 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc
     8581 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8701 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     8761 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     8821 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc
     8881 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     8941 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     9001 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     9061 tatcggctga atttgattgc gagtgagata tttatgcc
//
