LOCUS       sequence-32445-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, 
            Gage FH, Verma IM
  TITLE     Development of a novel mouse glioma model using lentiviral vectors.
  JOURNAL   Nat Med. 2009 Jan . 15(1):110-6.
  PUBMED    19122659
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2009 Jan . 15(1):110-6."
COMMENT     Sequence Label: pTomo
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..58
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    join(68..642,643..645,646..654)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(235..252)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     462..481
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     complement(700..721)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggccgccac tccaccggcg cctaaataac ttcgtataat gtatgctata cgaagttaat
       61 ggatccgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
      121 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
      181 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
      241 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
      301 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
      361 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
      421 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
      481 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
      541 ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
      601 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatggtg
      661 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      721 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
      781 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      841 accaccctga cctacg
//
