LOCUS       sequence-32447-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, 
            Gage FH, Verma IM
  TITLE     Development of a novel mouse glioma model using lentiviral vectors.
  JOURNAL   Nat Med. 2009 Jan . 15(1):110-6.
  PUBMED    19122659
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2009 Jan . 15(1):110-6."
COMMENT     Sequence Label: pTomo-AurA
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    24..56
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             74..97
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gccgccactc caccggcgcc taaataactt cgtataatgt atgctatacg aagttaatgg
       61 atccaccgcc atggactaca aggacgatga tgacaagggc gaattcatgg accgatctaa
      121 agaaaactgc atttcaggac ctgttaaggc tacagctcca gttggaggtc caaaacgtgt
      181 tctcgtgact cagcaatttc cttgtcagaa tccattacct gtaaatagtg gccaggctca
      241 gcgggtcttg tgtccttcaa attcttccca gcgcgttcct ttgcaagcac aaaagcttgt
      301 ctccagtcac aagccggttc agaatcagaa gcagaagcaa ttgcaggcaa ccagtgtacc
      361 tcatcctgtc tccaggccac tgaataacac ccaaaagagc aagcagcccc tgccatcggc
      421 acctgaaaat aatcctgagg aggaactggc atcaaaacag aaaaatgaag aatcaaaaaa
      481 gaggcagtgg gctttggaag actttgaaat tggtcgccct ctgggtaaag gaaagtttgg
      541 taatgtttat ttggcaagag aaaagcaaag caagtttatt ctggctctta aagtgttatt
      601 taaagctcag ctggagaaag ccggagtgga gcatcagctc agaagagaag tagaaataca
      661 gtcccacctt cggcatccta atattcttag actgtatggt tatttccatg atgctaccag
      721 agtctaccta attctggaat atgcaccact tggaacagtt tatagagaac ttcagaaact
      781 ttcaaagttt gatgagcaga gaactgctac ttatataaca gaattggcaa atgccctgtc
      841 ttactgtcat tcgaagagag ttattcatag agacattaag ccagagaact tacttcttg
//
