LOCUS       sequence-324471-       13291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the coding sequence of the
            Arabidopsis Copia93 retroelement EVADE (AT5G17125) lacking the 
            intron under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13291)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 13291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_EVDdi
FEATURES             Location/Qualifiers
     source          1..13291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        680..1024
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     968..988
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1133..1157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5077..5101)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5114..5123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5517..5696
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5738..6289
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      6309..6564
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6657..6681
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7209..8000
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8244..8832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8733..8752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8986..9003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9018..9158
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9084..9103)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9244..9266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9502..9696
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9762..10829)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(11263..11892)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13192..13216
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    join(13285..13291,1..14)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtatagaaaa gttgatttag gtgacactat agaatactca agctatgcat ccaacgcgtt
       61 gggagctctc ccatatggtc gagatctcct ttgccccgga gatcaccatg gacgactttc
      121 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
      181 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
      241 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
      301 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      361 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      421 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
      481 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
      541 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
      601 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
      661 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      721 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
      781 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
      841 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      901 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      961 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     1021 ggactgcagg acgatccgta tttttacaac aattaccaca acaaaacaaa caacaaacaa
     1081 cattacaatt tactattcta gtcgacctgc aggcggccgc actagtgata tcacaagttt
     1141 gtacaaaaaa gcaggcttca tggagacttc tcaaaagatg attactacgg tgatacttca
     1201 agggggaaac tacctaactt ggtcaagaac aacaaaaacg gtgttgtgtg gtcgtgggct
     1261 ttggagccat gtaatctcat ctaaagctcc aaaagaagcc aaagaagaag aagaaacgga
     1321 gactatatct cccgaggaag aaaaatggtt ccaagaggat caagctgtcc ttgctcttct
     1381 tcaaaactct cttgaaacct ctattctaga gggatactct tactgtgaga cagcaaaaga
     1441 gttgtgggat acacttaaaa acgtgtatgg gaacgaatcg aatttgaccc gcgtgtttga
     1501 agtgaagaag gcgattaacg aactaagtca agaagactta gagtttacta aacactttgg
     1561 taagttcagg tccttatggt ctgaacttga gagcttgagg ccgggaacgc ttgacccgaa
     1621 gattctccac gaaaggcgtg agcaagacaa ggtgtttgga ctccttctca ccttaaatcc
     1681 agggtacaat gacctaatta aacacttgct taggtcggag aagttaccaa gtcttgatga
     1741 ggtgtgttct aagattcaga aagagcaagg atcaaccggg ctatttggtg gaaaaagtga
     1801 actcatcacg gctaacaaag gagaagtagt ggcgaacaaa ggtgtgtaca agaatgagga
     1861 tagaaaactg ttgacttgtg atcattgtaa gaagaagggc cacaccaaag acaaatgttg
     1921 gttgctacat ccacacctca agcccgccaa gttcaaagac tcaagagctc atctctctca
     1981 agaaactcat gaggagcaaa gtcaagccgg gtcatcgaaa ggagagacat ccacatcatt
     2041 cggagactat gtgaggaagt cggacttgga agcactcatc aagtccattg cttcactcaa
     2101 agaatccgga tattgagacg ggaaaagtta ttggagaagg tggttcaaaa ggagaactct
     2161 atgttcttga agacttgtca ccaaactctt ctagctgttt ttcctctaag tcacatttag
     2221 gcatctcttt taatactttg tggcatgcta ggctaggtca tcctcatact agagctctta
     2281 aactcatgtt accaaatatt tcttttgatc atacaagttg tgaggcttgt attcttggta
     2341 aacattgcaa atcggttttt cctaaatctc ttactatcta tgagaaatgt tttgatctag
     2401 ttcattcgga tgtttggacc tcaccatgtg tttctagaga caacaacaaa tattttgtca
     2461 catttattga tgagaaatca aagtacactt ggataacact attaccctca aaagatagag
     2521 tgtttgaagc tttcactaac tttgaaacct atgtcactaa tcaatttaat gcaaagatta
     2581 aagtgtttag gaccgataat ggaggagaat acacaagcca aaagtttcga gatcatctag
     2641 ccaaacgtgg aataatacac caaacaagtt gcccgtacac accacaacaa aatggagtgg
     2701 cggagagaaa gaaccgacat ctcatggagg tggcgcggtc aatgatgttt catacaagtg
     2761 ttccaaagag attttggggt gatgcggtct taaccgcgtg ctaccttatc aatcgaacac
     2821 caactaaagt cttaagtgac ttatctccct tcgaggtact taacaatatt aaaccgttca
     2881 ttgatcactt acgagtgttt ggttgtgtat gttttgtttt gatacccgga gaacaaagaa
     2941 gcaagctaga tgcgaagagc actaagtgta tgtttcttgg ctactcaact actcaaaaag
     3001 gatataaatg ctttgatcca accaagaacc gcacatttat atcccgagat gtcaagtttc
     3061 ttgaaaacca agactacaac aacaagaagg attgggagaa cctaaaagat cttactcatt
     3121 ccacctccga tcgtgttgca actctaaagt ttctattgga tcatttgggc aatgactcaa
     3181 cttcgacaac tcaacatcaa ccggagatga cacaagatca agaagacttg aatcaagaga
     3241 atgaggaggt gtccctacaa catcaagaaa atcttacaca cgtgcaagaa gatcctccaa
     3301 acacacaaga acactctgaa catgttcaag aaatccaaga tgactcaagt gaagatgaag
     3361 aacgtacaca agttcttcca cctccaccac ctcttagaag gagtacacgc attcgacgca
     3421 agaaggaatt cttcaactct aatgcggtag ctcatccttt ccaagcgaca tgttctcttg
     3481 cccttgtacc tctagatcat caagcgtttc tctccaagat ttctgagcat cggattcctc
     3541 aaacatatga agaggcaatg gaagtcaagg aatggaggga tgcgatagcc gatgagataa
     3601 acgctatgaa acgaaatcat acttgggatg aagatgactt acctaaagga aagaaaacag
     3661 tgagctcaag gtgggtattc acaatcaagt ataagagtaa tggagatatc gagagatata
     3721 aaactcgctt ggtggctcgg gggttcacgc aaacatatgg ctccgactac atggagactt
     3781 ttgctccggt ggctaagcta cacacggtga gagtggttct tgccttagca acaaatttat
     3841 catggggact atggcaaatg gatgtcaaga atgcatttct ccaaggcgaa cttgaagatg
     3901 atgtgtatat gacgcctcca cccggtctag aagataccat tccatgtgat aaagtgttga
     3961 gactacgcaa agctatctat ggtcttaaac aatctccacg tgcatggtac cacaagctca
     4021 gccgaactct taaagatcat ggattcaaga agtccgagtc cgatcacact ttgtttacat
     4081 tacaaagtcc tcaaggtatt gtcgttgttc tcatctatgt tgatgatctt atcatcaccg
     4141 gagacaacaa agacggtata gactctacca agacttttct taaatcttgt tttgatatta
     4201 aagatcttgg tgaactcaaa tacttcctag gaattgaagt gtgtcgctct aatgctggac
     4261 tctttctatc acaacgcaaa tacactcttg acttgttgaa tgagacaggt tttatggacg
     4321 ctaaaccggc tcgcactcca cttgaagatg gctacaaggt caatcgaaag ggggagaaag
     4381 aagatgagaa gtttggcgat gcacctctct atcgcaaact tgtaggtaag ttgatctatt
     4441 tgacaaacac aagacctgac atttgttttg cggtaaatca ggttagtcaa cacatgaaag
     4501 tcccgatggt gtatcattgg aacatggtgg agagaatact aaggtatctc aaaggcagct
     4561 ccggacaagg catatggatg ggaaagaact caagcaccga gatagtggga tattgcgatg
     4621 cggactatgc gggtgataga ggagatagaa gatctacaac tggttattgt acgttcattg
     4681 gaggtaacct tgcaacttgg aagacaaaga aacagaaggt ggtctcatgt tcgagtgcag
     4741 aatcggagta tagagcaatg agaaagctca ccaacgaact cacttggctt aaagctcttc
     4801 ttaaagatgt tggcattgaa caacacatgc ctatcaccat gcattgtgac aacaaggcag
     4861 caatatacat agcctcaaat tcggtgtttc atgagagaac caagcatatt gaagtcgatt
     4921 gtcataaagt ccgagagaag atcatcgaag gagtcacact accgtgttat acaagaagtg
     4981 aagatcagtt ggcggatatc ttcaccaaag ccgcaagtct caaagtttgc aacttcatac
     5041 atggcaaatt aggacttgtg gatctatctc actcttaccc agctttcttg tacaaagtgg
     5101 tgatatcccg cggccatggc ggccgggagc atgcgacgtc gatctaactg actagccgcg
     5161 gccatgctag agtccgcaaa aatcaccagt ctctctctac aaatctatct ctctctattt
     5221 ttctccagaa taatgtgtga gtagttccca gataagggaa ttagggttct tatagggttt
     5281 cgctcatgtg ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc
     5341 tatcaataaa atttctaatt cctaaaacca aaatccagtg acctgcaggc atgcgacgtc
     5401 gggccctcta gaggatcccc gggtaccctc gaattatcat acatgagaat taagggagtc
     5461 acgttatgac ccccgccgat gacgcgggac aagccgtttt acgtttggaa ctgacagaac
     5521 cgcaacgttg aaggagccac tgagccgcgg gtttctggag tttaatgagc taagcacata
     5581 cgtcagaaac cattattgcg cgttcaaaag tcgcctaagg tcactatcag ctagcaaata
     5641 tttcttgtca aaaatgctcc actgacgttc cataaattcc cctcggtatc caattagagt
     5701 ctcatattca ctctcaactc gatcgagggg atctaccatg agcccagaac gacgcccggc
     5761 cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg tcaaccacta
     5821 catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg agtggacgga
     5881 cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg acggcgaggt
     5941 cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac gcctacgact ggacggccga
     6001 gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca cgctctacac
     6061 ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg tcatcgggct
     6121 gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc gcggcatgct
     6181 gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc agctggactt
     6241 cagcctgccg gtgccgcccc gtccggtcct gcccgtcacc gaaatctgat gacccctaga
     6301 gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
     6361 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
     6421 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
     6481 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
     6541 tgtcatctac tatgttacta gatcgaccgg catgcaagct gataattcaa ttcggcgtta
     6601 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     6661 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     6721 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     6781 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     6841 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     6901 gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg acataagcct cgttcggttc
     6961 gtaagctgta atgcaagtag cgtaactgcc gtcacgcaac tggtccagaa ccttgaccga
     7021 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tcttgttatg acatgttttt
     7081 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     7141 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     7201 taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     7261 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     7321 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     7381 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     7441 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     7501 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     7561 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     7621 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     7681 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     7741 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     7801 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     7861 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     7921 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     7981 ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcc
     8041 ggcgttaact caagcgatta gatgcactaa gcacataatt gctcacagcc aaactatcag
     8101 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     8161 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8221 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8281 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8341 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8401 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8461 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8521 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8581 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8641 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8701 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8761 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8821 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8881 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8941 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9001 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     9061 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9121 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     9181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     9241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     9301 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     9361 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     9421 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     9481 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     9541 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     9601 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     9661 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     9721 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     9781 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     9841 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     9901 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     9961 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    10021 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    10081 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    10141 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    10201 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    10261 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    10321 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    10381 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    10441 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    10501 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    10561 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    10621 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    10681 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    10741 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    10801 atttgtaccg ggccggatgg tttgcgaccg tcacgccgat tcctcgggct tgggggttcc
    10861 agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc
    10921 tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc
    10981 tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct
    11041 gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct
    11101 tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt
    11161 ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta
    11221 gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt
    11281 aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag
    11341 aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc
    11401 aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc
    11461 tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg
    11521 ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac
    11581 cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg
    11641 cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt
    11701 cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg
    11761 ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc
    11821 gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc
    11881 gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca
    11941 gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc
    12001 tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg
    12061 gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg
    12121 gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc
    12181 tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg
    12241 cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt
    12301 cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg
    12361 cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg
    12421 gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc
    12481 tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca
    12541 acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc
    12601 ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca
    12661 ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg
    12721 ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc
    12781 cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg
    12841 catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt
    12901 cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca
    12961 gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct
    13021 gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt
    13081 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
    13141 ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta ggtttacccg
    13201 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
    13261 caagctcaag ctaagcttga gctccaactt t
//
