LOCUS       sequence-324472-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp3 -EGFP
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6076..6128
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      6136..6145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6142..6144,6145..6147,6148..6861)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6142..6144,6145..6147,6148..6858)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6187..6208)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6448..6467)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6795..6816
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6984..7105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7021..7040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7075..7094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7112..7567)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7249..7270)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7461..7480
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7594..7698
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7700..8057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7700..7891
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7723..7743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7908..8043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8092..8886
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8092..8886
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8146..8165)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8756..8775
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9074..9093)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9118..9165
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9494..10082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9983..10002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10257..10560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta ggccaccatg
      241 gcccctacca aggtgacctt cggagacgac accgtgatcg aggtgcaggg ttacaagagc
      301 gtgaacatca ccttcgagct ggacgaacgc atcgacaagg tgctgaacga aaagtgctcc
      361 gcttacaccg tggagctggg caccgaggtg aacgaattcg cctgcgtggt ggccgacgct
      421 gtgatcaaga ccctgcagcc agtgtccgaa ctgctgaccc ctctgggtat cgacctggac
      481 gagtggtcta tggctaccta ctacctgttc gacgagagcg gcgaattcaa gctggcctct
      541 cacatgtact gcagcttcta ccctcccgac gaagacgagg aagagggtga ctgcgaagag
      601 gaagagttcg agccttccac tcagtacgag tacggcaccg aagacgacta ccagggcaag
      661 cctctggaat tcggagccac ctctgccgct ctgcagcctg aagaggaaca ggaggaagac
      721 tggctggacg acgactccca gcagaccgtg ggtcagcagg acggctctga agacaaccag
      781 accaccacca tccagaccat cgtggaggtg cagcctcagc tggagatgga actgacccca
      841 gtggtgcaga ccatcgaggt gaacagcttc tccggctacc tgaagctgac cgacaacgtg
      901 tacatcaaga acgctgacat cgtggaggaa gccaagaagg tgaaaccaac cgtggtggtg
      961 aacgccgcta acgtgtacct gaagcacggc ggaggtgtgg ctggagctct gaacaaggct
     1021 accaacaacg ccatgcaggt ggaatccgac gactacatcg ctaccaacgg tcctctgaag
     1081 gtgggcggat cctgcgtgct gtctggccac aacctggcca agcactgcct gcacgtggtg
     1141 ggaccaaacg tgaacaaggg agaggacatc cagctgctga agagcgccta cgaaaacttc
     1201 aaccagcacg aggtgctgct ggctccactg ctgtctgccg gaatcttcgg tgctgatccc
     1261 atccacagcc tgcgcgtgtg cgtggacacc gtgaggacca acgtgtacct ggccgtgttc
     1321 gacaagaacc tgtacgacaa gctggtgagc tccttcctgg agatgaagtc cgaaaagcag
     1381 gtggagcaga agatcgccga gatccccaag gaggaagtga agcctttcat caccgaatct
     1441 aagcccagcg tggagcagag aaagcaggac gacaagaaga tcaaggcctg cgtggaggaa
     1501 gtgaccacca ccctggagga aaccaagttc ctgaccgaga acctgctgct gtacatcgac
     1561 atcaacggca acctgcaccc tgactccgct accctggtgt ctgacatcga catcaccttc
     1621 ctgaagaagg acgcccccta catcgtgggc gacgtggtgc aggaaggagt gctgaccgct
     1681 gtggtcatcc ctaccaagaa ggccggtggc accaccgaga tgctggccaa ggctctgcgt
     1741 aaggtgccaa ccgacaacta catcaccacc taccccggtc agggcctgaa cggttacacc
     1801 gtggaggaag ctaagaccgt gctgaagaag tgcaagtctg ccttctacat cctgccctcc
     1861 atcatctcta acgagaagca ggaaatcctg ggcaccgtga gctggaacct gcgcgaaatg
     1921 ctggcccacg ctgaggaaac caggaagctg atgcccgtgt gcgtggagac caaggccatc
     1981 gtgagcacca tccagagaaa gtacaagggc atcaagatcc aggagggtgt ggtggactac
     2041 ggcgctcgtt tctacttcta cacctccaag accaccgtgg cctctctgat caacaccctg
     2101 aacgacctga acgagaccct ggtgaccatg ccactgggat acgtgaccca cggtctgaac
     2161 ctggaggaag ccgctagata catgcgttcc ctgaaggtgc ctgctaccgt gagcgtgagc
     2221 agccctgacg ctgtgaccgc ctacaacgga tacctgacct cctctagcaa gacccctgag
     2281 gaacacttca tcgaaaccat cagcctggcc ggttcctaca aggactggtc ttacagcgga
     2341 cagtccaccc agctgggcat cgagttcctg aagcgcggag acaagagcgt gtactacacc
     2401 tccaacccaa ccaccttcca cctggacggt gaagtgatca ccttcgacaa cctgaagacc
     2461 ctgctgtctc tgcgcgaggt gaggaccatc aaggtgttca ccaccgtgga caacatcaac
     2521 ctgcacaccc aggtggtgga catgagcatg acctacggac agcagttcgg tcctacctac
     2581 ctggacggag ctgacgtgac caagatcaag ccacacaact cccacgaggg caagaccttc
     2641 tacgtgctgc ctaacgatga caccctgaga gtggaggctt tcgaatacta ccacaccacc
     2701 gacccatcct tcctgggcag gtacatgtct gccctgaacc acaccaagaa gtggaagtac
     2761 ccccaggtga acggactgac cagcatcaag tgggccgaca acaactgcta cctggccacc
     2821 gctctgctga ccctgcagca gatcgaactg aagttcaacc cacccgctct gcaggacgct
     2881 tactacagag cccgtgctgg cgaggccgct aacttctgcg ctctgatcct ggcctactgc
     2941 aacaagaccg tgggcgaact gggagacgtg agagagacca tgagctacct gttccagcac
     3001 gccaacctgg actcctgcaa gcgtgtgctg aacgtggtgt gcaagacctg cggccagcag
     3061 cagaccaccc tgaagggagt ggaagctgtg atgtacatgg gtaccctgtc ctacgagcag
     3121 ttcaagaagg gcgtgcagat cccttgcacc tgcggaaagc aggccaccaa gtacctggtg
     3181 cagcaggaaa gcccattcgt gatgatgtcc gctcctccag cccagtacga actgaagcac
     3241 ggcaccttca cctgcgcttc cgagtacacc ggcaactacc agtgcggaca ctacaagcac
     3301 atcacctcta aggagaccct gtactgcatc gacggagccc tgctgaccaa gtcctctgaa
     3361 tacaagggtc ccatcaccga cgtgttctac aaggagaaca gctacaccac caccatcaag
     3421 cccgtgacct acaagctgga cggcgtggtg tgcaccgaaa tcgaccctaa gctggacaac
     3481 tactacaaga aggacaactc ttacttcacc gagcagccta tcgacctggt gccaaaccag
     3541 ccctacccta acgccagctt cgacaacttc aagttcgtgt gcgacaacat caagttcgct
     3601 gacgacctga accagctgac cggatacaag aagcctgcct ctagagagct gaaggtgacc
     3661 ttcttcccag acctgaacgg cgacgtggtg gctatcgact acaagcacta caccccaagc
     3721 ttcaagaagg gagccaagct gctgcacaag cccatcgtgt ggcacgtgaa caacgctacc
     3781 aacaaggcca cctacaagcc taacacctgg tgcatccgtt gcctgtggtc taccaagcca
     3841 gtggaaacct ccaactcttt cgacgtgctg aagagcgagg acgctcaggg catggacaac
     3901 ctggcctgcg aagacctgaa gcccgtgagc gaggaagtgg tggagaaccc aaccatccag
     3961 aaggacgtgc tggaatgcaa cgtgaagacc accgaggtgg tgggagacat catcctgaag
     4021 ccagctaaca acagcctgaa gatcaccgag gaagtgggtc acaccgacct gatggccgct
     4081 tacgtggaca acagctccct gaccatcaag aagcccaacg agctgagcag ggtgctgggt
     4141 ctgaagaccc tggctactca cggtctggct gctgtgaact ctgtgccatg ggacaccatc
     4201 gctaactacg ccaagccttt cctgaacaag gtggtgtcca ccaccaccaa catcgtgacc
     4261 agatgtctga accgcgtgtg caccaactac atgccctact tcttcaccct gctgctgcag
     4321 ctgtgcacct tcaccagaag caccaactcc cgtatcaagg ctagcatgcc taccaccatc
     4381 gccaagaaca ccgtgaagag cgtgggaaag ttctgcctgg aggcttcctt caactacctg
     4441 aagtctccaa acttcagcaa gctgatcaac atcatcatct ggttcctgct gctctccgtg
     4501 tgcctgggtt ccctgatcta ctctaccgcc gctctgggag tgctgatgtc taacctgggt
     4561 atgcctagct actgcactgg ttacagagag ggatacctga acagcaccaa cgtgaccatc
     4621 gccacctact gcaccggcag catcccatgc tccgtgtgcc tgtctggact ggacagcctg
     4681 gacacctacc cctccctgga aaccatccag atcaccatct ctagcttcaa gtgggacctg
     4741 accgccttcg gtctggtggc tgagtggttc ctggcctaca tcctgttcac caggttcttc
     4801 tacgtgctgg gcctggccgc tatcatgcag ctgttcttct cctacttcgc tgtgcacttc
     4861 atcagcaact cctggctgat gtggctgatc atcaacctgg tgcagatggc tcccatctct
     4921 gccatggtgc gcatgtacat cttcttcgcc agcttctact acgtgtggaa gtcctacgtg
     4981 cacgtggtgg acggttgcaa ctcctctacc tgcatgatgt gctacaagcg caacagggct
     5041 accagggtgg agtgcaccac catcgtgaac ggcgtgcgca ggtccttcta cgtgtacgcc
     5101 aacggcggca agggattctg caagctgcac aactggaact gcgtgaactg cgacaccttc
     5161 tgcgctggct ccaccttcat ctctgacgag gtggccagag acctgagcct gcagttcaag
     5221 cgtccaatca accccaccga ccagagctcc tacatcgtgg actctgtgac cgtgaagaac
     5281 ggtagcatcc acctgtactt cgacaaggct ggccagaaga cctacgagcg ccactctctg
     5341 agccacttcg tgaacctgga caacctgagg gccaacaaca ccaagggcag cctgcctatc
     5401 aacgtgatcg tgttcgacgg aaagtccaag tgcgaggaat ctagcgccaa gtccgccagc
     5461 gtgtactaca gccagctgat gtgccagcca atcctgctgc tggaccaggc tctggtgtct
     5521 gacgtgggag acagcgccga agtggccgtg aagatgttcg acgcctacgt gaacaccttc
     5581 tcctctacct tcaacgtgcc tatggagaag ctgaagaccc tggtggctac tgctgaggct
     5641 gaactggcta agaacgtgtc cctggacaac gtgctgagca ccttcatctc cgccgctagg
     5701 cagggtttcg tggactctga cgtggaaacc aaagacgtgg tggagtgcct gaagctgtct
     5761 caccagagcg acatcgaagt gaccggcgac tcctgcaaca actacatgct gacctacaac
     5821 aaggtggaga acatgacccc aagagacctg ggagcttgca tcgactgctc cgcccgtcac
     5881 atcaacgctc aggtggccaa gtctcacaac atcgccctga tctggaacgt gaaggacttc
     5941 atgagcctgt ccgagcagct gcgcaagcag atcaggagcg ccgctaagaa gaacaacctg
     6001 cccttcaagc tgacctgcgc taccaccaga caggtggtga acgtggtgac caccaagatc
     6061 gccctgaagg gcggactcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     6121 cgggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     6181 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     6241 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     6301 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     6361 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6421 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     6481 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     6541 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     6601 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     6661 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     6721 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     6781 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     6841 atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat
     6901 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     6961 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     7021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     7081 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     7141 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     7201 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     7261 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     7321 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     7381 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     7441 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     7501 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     7561 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     7621 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     7681 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     7741 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7801 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7861 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7981 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8041 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     8101 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     8161 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     8221 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     8281 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     8341 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     8401 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     8461 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     8521 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     8581 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     8641 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     8701 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     8761 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     8821 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     8881 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     8941 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     9001 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     9061 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     9121 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     9181 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     9241 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     9301 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     9361 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     9421 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     9481 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     9541 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     9601 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9661 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9721 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9781 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9841 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9901 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9961 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
    10021 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
    10081 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
    10141 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcattagt
    10201 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
    10261 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    10321 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    10381 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    10441 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    10501 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
