LOCUS       sequence-324475-       11934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing mGFP5-GUS fusion reporter 
            under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 11934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_GFP-GUS
FEATURES             Location/Qualifiers
     source          1..11934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..33
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     15..32
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        680..1024
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     968..988
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1133..1157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1188..1216)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1811..1831
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1908..3719
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(3720..3744)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3757..3766
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4160..4339
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4381..4932
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4952..5207
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5300..5324
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5852..6643
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6887..7475
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7376..7395
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7629..7646
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7661..7801
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7727..7746)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7887..7909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8145..8339
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8405..9472)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(9906..10535)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11835..11859
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    join(11928..11934,1..14)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtatagaaaa gttgatttag gtgacactat agaatactca agctatgcat ccaacgcgtt
       61 gggagctctc ccatatggtc gagatctcct ttgccccgga gatcaccatg gacgactttc
      121 tctatctcta cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca
      181 ccgataatga gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta
      241 gagaggccta cgcggcaggt ctcatcaaga cgatctaccc gagtaataat ctccaggaga
      301 tcaaatacct tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca
      361 agaacacaga gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc
      421 aaggcttgct tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc
      481 ctactgaatc aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg
      541 tgaagactgg cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct
      601 tcgtcaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
      661 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
      721 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
      781 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
      841 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
      901 ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac
      961 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
     1021 ggactgcagg acgatccgta tttttacaac aattaccaca acaaaacaaa caacaaacaa
     1081 cattacaatt tactattcta gtcgacctgc aggcggccgc actagtgata tcacaagttt
     1141 gtacaaaaaa gcaggctatg agtaaaggag aagaactttt cactggagtt gtcccaattc
     1201 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1261 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1321 ttccatggcc aaccctggtc accaccctga cctacggcgt gcagtgcttc tcccgttacc
     1381 ctgatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg
     1441 agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg
     1501 agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa
     1561 acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg
     1621 acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc gaagacggcg
     1681 gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1741 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1801 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggtag
     1861 aactatacaa actcgaggca gctgcagctg cagctgcagc tcctaggatg ttacgtcctg
     1921 tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc
     1981 gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa
     2041 ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgcgg
     2101 gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg
     2161 tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga
     2221 tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg
     2281 ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc
     2341 cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc catgatttct
     2401 ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac acctgggtgg
     2461 acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct gttgactggc
     2521 aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa caggtggttg
     2581 caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc tggcaaccgg
     2641 gtgaaggtta tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct
     2701 acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc
     2761 acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggacttg cgtggcaaag
     2821 gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact
     2881 cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg
     2941 gcatcgtggt gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt
     3001 tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc
     3061 agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg
     3121 tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgggaatatt
     3181 tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca
     3241 atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc
     3301 tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac
     3361 tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat
     3421 acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt
     3481 atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg
     3541 gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg
     3601 gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc
     3661 aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa
     3721 cccagctttc ttgtacaaag tggtgatatc ccgcggccat ggcggccggg agcatgcgac
     3781 gtcgatctaa ctgactagcc gcggccatgc tagagtccgc aaaaatcacc agtctctctc
     3841 tacaaatcta tctctctcta tttttctcca gaataatgtg tgagtagttc ccagataagg
     3901 gaattagggt tcttataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     3961 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     4021 gtgacctgca ggcatgcgac gtcgggccct ctagaggatc cccgggtacc ctcgaattat
     4081 catacatgag aattaaggga gtcacgttat gacccccgcc gatgacgcgg gacaagccgt
     4141 tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactgagccg cgggtttctg
     4201 gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa aagtcgccta
     4261 aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg ttccataaat
     4321 tcccctcggt atccaattag agtctcatat tcactctcaa ctcgatcgag gggatctacc
     4381 atgagcccag aacgacgccc ggccgacatc cgccgtgcca ccgaggcgga catgccggcg
     4441 gtctgcacca tcgtcaacca ctacatcgag acaagcacgg tcaacttccg taccgagccg
     4501 caggaaccgc aggagtggac ggacgacctc gtccgtctgc gggagcgcta tccctggctc
     4561 gtcgccgagg tggacggcga ggtcgccggc atcgcctacg cgggtccctg gaaggcacgc
     4621 aacgcctacg actggacggc cgagtcgacc gtgtacgtct ccccccgcca ccagcggacg
     4681 ggactgggct ccacgctcta cacccacctg ctgaagtccc tggaggcaca gggcttcaag
     4741 agcgtggtcg ctgtcatcgg gctgcccaac gacccgagcg tgcgcatgca cgaggcgctc
     4801 ggatatgccc cccgcggcat gctgcgggcg gccggcttca agcacgggaa ctggcatgac
     4861 gtgggtttct ggcagctgga cttcagcctg ccggtgccgc cccgtccggt cctgcccgtc
     4921 accgaaatct gatgacccct agagtcaagc agatcgttca aacatttggc aataaagttt
     4981 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     5041 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     5101 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     5161 ctaggataaa ttatcgcgcg cggtgtcatc tactatgtta ctagatcgac cggcatgcaa
     5221 gctgataatt caattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     5281 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     5341 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     5401 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     5461 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     5521 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     5581 tggacataag cctcgttcgg ttcgtaagct gtaatgcaag tagcgtaact gccgtcacgc
     5641 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     5701 gcttcttgtt atgacatgtt tttttggggt acagtctatg cctcgggcat ccaagcagca
     5761 agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca
     5821 gggcagtcgc cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc
     5881 gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc
     5941 cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt
     6001 gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct
     6061 tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat
     6121 tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg
     6181 agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga
     6241 tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc
     6301 ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt
     6361 aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt
     6421 gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga
     6481 ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc
     6541 ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt
     6601 ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc
     6661 gttcaagccg acgccgcttc gccggcgtta actcaagcga ttagatgcac taagcacata
     6721 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     6781 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     6841 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6901 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6961 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7021 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7081 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7141 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7201 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7261 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7321 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7381 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7441 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7501 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7561 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7621 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     7681 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     7741 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     7801 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     7861 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     7921 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     7981 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8041 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8101 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8161 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8221 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8281 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     8341 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     8401 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     8461 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     8521 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     8581 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     8641 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     8701 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     8761 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     8821 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     8881 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     8941 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9001 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9061 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9121 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9181 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9241 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9301 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
     9361 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
     9421 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgtcacgcc
     9481 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
     9541 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     9601 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     9661 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     9721 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     9781 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     9841 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     9901 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     9961 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    10021 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    10081 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    10141 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    10201 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    10261 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    10321 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    10381 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    10441 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    10501 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    10561 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
    10621 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
    10681 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
    10741 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
    10801 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
    10861 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
    10921 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
    10981 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
    11041 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
    11101 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
    11161 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
    11221 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
    11281 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
    11341 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
    11401 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
    11461 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
    11521 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
    11581 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
    11641 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
    11701 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
    11761 aaatggacga acggataaac cttttcacgc ccttttaaat atccgttatt ctaataaacg
    11821 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga
    11881 aggcgggaaa cgacaatctg atccaagctc aagctaagct tgagctccaa cttt
//
